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    Methods for the production of insulin in plants
    5.
    发明申请
    Methods for the production of insulin in plants 失效
    在植物中生产胰岛素的方法

    公开(公告)号：US20050039235A1

    公开(公告)日：2005-02-17

    申请号：US10869040

    申请日：2004-06-17

    申请人： Maurice Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

    发明人： Maurice Moloney ,  Joseph Boothe ,  Richard Keon ,  Cory Nykiforuk ,  Gijs Van Rooijen

    IPC分类号： A01H1/00 ,  C07K14/62 ,  C12N15/82

    CPC分类号： C07K14/62 ,  C12N15/8257

    摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5′ to 3′ direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

    摘要翻译： 描述了在植物中产生胰岛素的方法。 在一个实施方案中，本发明提供了一种用于在植物中表达胰岛素的方法，包括：（a）提供嵌合核酸构建体，其以5'至3'的转录方向包含可操作地连接的组分：（i）核酸 能够控制植物种子细胞中表达的序列; 和（ii）编码胰岛素多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 和（c）将植物细胞生长成能种植种子的成熟植物，其中种子表达胰岛素。

    Oil bodies and associated proteins as affinity matrices
    6.
    发明授权
    Oil bodies and associated proteins as affinity matrices 失效
    油体和相关蛋白质作为亲和基质

    公开(公告)号：US06509453B1

    公开(公告)日：2003-01-21

    申请号：US09319275

    申请日：1999-08-27

    申请人： Maurice Moloney ,  Joseph Boothe ,  Gijs Van Rooijen

    发明人： Maurice Moloney ,  Joseph Boothe ,  Gijs Van Rooijen

    IPC分类号： C07K100

    CPC分类号： C07H21/00 ,  B01D15/08 ,  B01D15/3804 ,  B01D15/3809 ,  C01G11/003 ,  C07K1/22 ,  C07K14/415 ,  C07K16/065 ,  C08B1/00 ,  C12N9/6429 ,  C12Y304/21005

    摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions

    摘要翻译： 描述了从混合物中分离靶分子的方法。 该方法使用油体及其相关蛋白作为所需靶分子的选择性，非共价结合的亲和基质。 油体蛋白质可以遗传融合到对所需靶分子具有特异性的配体。 天然油体蛋白质也可以与油体蛋白质特异性配体如抗体或油体结合蛋白结合使用。 该方法允许由于油体和水溶液之间的密度差而使所需目标分子分离和回收

    Oil bodies and associated proteins as affinity matrices
    7.
    发明授权
    Oil bodies and associated proteins as affinity matrices 失效
    油体和相关蛋白质作为亲和基质

    公开(公告)号：US07332587B2

    公开(公告)日：2008-02-19

    申请号：US10260960

    申请日：2002-10-01

    申请人： Maurice Moloney ,  Joseph Boothe ,  Gijs Van Rooijen

    发明人： Maurice Moloney ,  Joseph Boothe ,  Gijs Van Rooijen

    IPC分类号： A23J1/00

    CPC分类号： C07H21/00 ,  B01D15/08 ,  B01D15/3804 ,  B01D15/3809 ,  C01G11/003 ,  C07K1/22 ,  C07K14/415 ,  C07K16/065 ,  C08B1/00 ,  C12N9/6429 ,  C12Y304/21005

    摘要： A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.

    摘要翻译： 描述了从混合物中分离靶分子的方法。 该方法使用油体及其相关蛋白作为所需靶分子的选择性，非共价结合的亲和基质。 油体蛋白质可以遗传融合到对所需靶分子具有特异性的配体。 天然油体蛋白质也可以与油体蛋白质特异性配体如抗体或油体结合蛋白结合使用。 该方法允许由于油体和水溶液之间的密度差而使所需目标分子分离和回收。