LAYERED BODY
    3.
    发明申请

    公开(公告)号:US20210147795A1

    公开(公告)日:2021-05-20

    申请号:US17094935

    申请日:2020-11-11

    IPC分类号: C12N5/00 G01N33/50 G01N21/64

    摘要: An object of the present invention is to provide a layered body of cells that is used in a co-culture technique and that is capable of recognizing as paracrine effect of each cell and detecting the effect with high intensity. The layered body of the present invention is a layered body 1A having a layered structure in which a gel layer 20a containing a hydrogel is disposed between at least two cell layers 10a and 10b containing cells of different types from each other, wherein the hydrogel is a multi-branched polymer hydrogel formed by a reaction of: Liquid A containing a multi-branched polymer A, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more electrophilic functional groups in at least one of a side chain(s) and an end(s); and Liquid B containing a multi-branched polymer B, the polymer containing, as a backbone, a polyethylene glycol containing at least three branches, the branches containing one or more nucleophilic functional groups in at least one of a side chain(s) and an end(s), the concentration of components derived from the multi-branched polymers A and B in the hydrogel is from 0.6 to 8% by weight, and the thickness is from 0.02 mm to 2 mm.