摘要:
A synthetic gene encoding an insecticidal protein, the gene is provided having a base sequence which has been modified to bring the codon usage in conformity with the genes of gramineous plants particularly rice plants. A preferred gene sequence is shown in SEQ ID NO: 1. An expression vector containing said structural gene is provided. A method for producing recombinant gramineous plants is provided, which comprises transforming protoplasts derived from gramineous plants with the vector, incubating the protoplasts in a liquid medium in the presence of cultured cells of the plants to form colonies and regenerating plants from said colonies. Recombinant gramineous plants having resistance against insects are also provided.
摘要翻译:编码杀虫蛋白的合成基因,提供了具有经修饰以使密码子使用与禾本科植物特别是水稻植物基因一致的碱基序列的基因。 优选的基因序列示于SEQ ID NO:1中。提供含有所述结构基因的表达载体。 提供了一种生产重组禾本科植物的方法,其包括用载体转化源自禾本科植物的原生质体,在培养的植物细胞存在下在液体培养基中孵育原生质体以形成菌落并从所述菌落再生植物。 还提供了具有抗昆虫的重组禾本科植物。
摘要:
The object of the present invention is to isolate Rf gene, particularly Rf1 gene derived from radish, and identify its structure. The present invention provides a protein involved in restoration of a cytoplasmic male sterile individual to fertility which has 14 or more pentatricopeptide repeat (hereafter may be abbreviated to PPR) motifs, wherein a group of the motifs is divided into 3 or more blocks, each of the individual blocks has at least 2 or more PPR motifs, and the block in a carboxyl terminal (C terminal) side has 4 PPR motifs.
摘要:
The object of the present invention is to isolate Rf gene, particularly Rf1 gene derived from radish, and identify its structure. The present invention provides a protein involved in restoration of a cytoplasmic male sterile individual to fertility which has 14 or more pentatricopeptide repeat (hereafter may be abbreviated to PPR) motifs, wherein a group of the motifs is divided into 3 or more blocks, each of the individual blocks has at least 2 or more PPR motifs, and the block in a carboxyl terminal (C terminal) side has 4 PPR motifs.
摘要:
A novel electromagnetic balance-type weighing apparatus according to the invention includes a weighing pan/balance beam assembly (15) whose balance beam is angularly displaced as a function of the weight of an object of weighing placed on the weighing pan. An electromagnetic coil (9) is provided to generate an electromagnetic force that offsets the displacement of the balance beam of the weighing pan/balance beam assembly (15) caused by the weight of the object of weighing. A displacement detector (3, 4, 5) is provided to detect the displacement of the balance beam of the weighing pan/balance beam assembly (15). An operation unit (7) performs operations to determine a control voltage in response to the detection signal transmitted from the displacement detector (3, 4, 5). A current transducer circuit (20) generates an electric current in response to the control voltage applied to it by the operation unit (7) and supplies it to the electromagnetic coil (9). The current transducer circuit (20) comprises a negative feedback loop circuit for feeding the output current from said current transducer circuit (20) back to the input terminal of the current transducer circuit (20). A weight calculator (24) calculates the weight of the object of weighing from the output current of the current transducer circuit (20).
摘要:
An inverter controlled conveying/measuring apparatus according to the invention includes a conveyor (3) for conveying an object (W) of measurement. The conveyor (3) is driven by an AC motor (M2). Inverter (20) controls and operates the conveyor (3) at a given speed by supplying the AC motor (M2) with frequency converting signals according to a specified frequency. A measuring section (4) is arranged on a conveying path of the conveyor (3) in order to measure an attribute of the object (W) being conveyed by the conveyor (3).
摘要:
Various highly sensitive detection methods, particularly improved PNA-LNA-PCR clamp methods, are provided as methods for detecting the presence or absence of a mutated gene contained in a gene pool rapidly, in a simple manner, with high accuracy, and with high sensitivity. As a step before the main step for detection, a pre-amplification step comprising allowing (1) a clamp primer consisting of PNA which hybridizes with all or part of a target site having a sequence of a wild-type gene or a sequence complementary to the wild-type gene, (2) a primer capable of amplifying a region comprising a target site having a sequence of the mutated gene, and (3) the gene pool to coexist in a reaction solution for gene amplification, and selectively amplifying the region comprising a target site of the mutated gene by a gene amplification method.
摘要:
The object of the present invention is to isolate Rf gene, particularly Rf1 gene derived from radish, and identify its structure. The present invention provides a protein involved in restoration of a cytoplasmic male sterile individual to fertility which has 14 or more pentatricopeptide repeat (hereafter may be abbreviated to PPR) motifs, wherein a group of the motifs is divided into 3 or more blocks, each of the individual blocks has at least 2 or more PPR motifs, and the block in a carboxyl terminal (C terminal) side has 4 PPR motifs.