公开(公告)号：US20130052714A1

公开(公告)日：2013-02-28

申请号：US13643905

申请日：2011-04-25

申请人： Hideyuki Kumita ,  Daisuke Yamaguchi ,  Yuichi Tokita ,  Shuji Fujita

发明人： Hideyuki Kumita ,  Daisuke Yamaguchi ,  Yuichi Tokita ,  Shuji Fujita

IPC分类号： C12N9/02

CPC分类号： C12Y103/03005 ,  C12N9/001

摘要： Disclosed is a method of producing a recombinant bilirubin oxidase, including a step wherein a bilirubin oxidase gene derived from imperfect filamentous fungus Myrothecium verrucaria is introduced to Escherichia coli and expression thereof is allowed. Also disclosed is a recombinant bilirubin oxidase obtained by this production method. This recombinant bilirubin oxidase has an enzymatic activity higher than that of a natural bilirubin oxidase derived from the imperfect filamentous fungus Myrothecium verrucaria.

摘要翻译： 本发明公开了一种重组胆红素氧化酶的制备方法，其特征在于，将来自不完全丝状真菌疣状疟原虫的胆红素氧化酶基因导入大肠杆菌并进行表达。 还公开了通过该制造方法获得的重组胆红素氧化酶。 这种重组胆红素氧化酶的酶活性高于源自不完美丝状真菌疣状疣虫的天然胆红素氧化酶。

公开(公告)号：US08841036B2

公开(公告)日：2014-09-23

申请号：US13087470

申请日：2011-04-15

申请人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

发明人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

IPC分类号： H01M8/04 ,  H01M8/24 ,  H01M2/38 ,  H01M2/40 ,  H01M8/16

CPC分类号： H01M8/16 ,  Y02E60/527

摘要： Disclosed herein is a flexible fuel cell including, one or a plurality of cell sections, and a sealing sheet covering the cell section or sections, wherein the cell section has, at least, a pair of electrode sheets which form an anode and a cathode and at least one of which is accompanied by an oxidoreductase present at a surface thereof, a separator which is disposed between the electrode sheets and which has a proton-permeable membrane, a pair of current collectors which are electrically conductively connected respectively to the electrode sheets with a conductive adhesive, and a fuel reservoir section which is provided at such a position as to make contact with the anode at least and in which a fuel solution containing a fuel component is reserved.

摘要翻译： 本文公开了一种柔性燃料电池，其包括一个或多个电池部分，以及覆盖电池部分的密封片，其中电池部分至少具有一对形成阳极和阴极的电极片， 其中至少一个伴随着存在于其表面的氧化还原酶，隔离物设置在电极片之间并具有质子渗透膜，一对集电体分别与电极片电连接， 导电粘合剂和燃料储存部分，其设置在至少与阳极接触的位置处，并且其中保留了含有燃料部件的燃料溶液。

公开(公告)号：US20110287328A1

公开(公告)日：2011-11-24

申请号：US13087470

申请日：2011-04-15

申请人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

发明人： Shuji Fujita ,  Takaaki Nakagawa ,  Ryuhei Matsumoto ,  Hideyuki Kumita ,  Daisuke Yamaguchi

IPC分类号： H01M8/16

CPC分类号： H01M8/16 ,  Y02E60/527

摘要： Disclosed herein is a flexible fuel cell including, one or a plurality of cell sections, and a sealing sheet covering the cell section or sections, wherein the cell section has, at least, a pair of electrode sheets which form an anode and a cathode and at least one of which is accompanied by an oxidoreductase present at a surface thereof, a separator which is disposed between the electrode sheets and which has a proton-permeable membrane, a pair of current collectors which are electrically conductively connected respectively to the electrode sheets with a conductive adhesive, and a fuel reservoir section which is provided at such a position as to make contact with the anode at least and in which a fuel solution containing a fuel component is reserved.

摘要翻译： 本文公开了一种柔性燃料电池，其包括一个或多个电池部分，以及覆盖电池部分的密封片，其中电池部分至少具有一对形成阳极和阴极的电极片， 其中至少一个伴随着存在于其表面的氧化还原酶，隔离物设置在电极片之间并具有质子渗透膜，一对集电体分别与电极片电连接， 导电粘合剂和燃料储存部分，其设置在至少与阳极接触的位置处，并且其中保留了含有燃料部件的燃料溶液。

公开(公告)号：US20130130130A1

公开(公告)日：2013-05-23

申请号：US13699061

申请日：2011-04-25

申请人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

发明人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

IPC分类号： H01M8/04

CPC分类号： H01M8/04201 ,  H01M8/04082 ,  H01M8/16 ,  H01M8/2455 ,  Y02E60/527

摘要： The valve control unit of a fuel leakage preventing structure of the present invention includes first and second electrodes. The first electrode is attached to a first member that is movable. When a pressing force is applied to the first member to move, the first and second electrodes are brought into contact with each other, to cause conduction. The first and second electrodes are connected to a control device, and the control device opens a control valve when the first and second electrodes are put into a conducting state. As the control valve is opened and closed by a pressing force in this manner, a fuel solution can be easily supplied.

摘要翻译： 本发明的燃料泄漏防止结构的阀门控制单元包括第一和第二电极。 第一电极附接到可移动的第一构件。 当对第一构件施加按压力以移动时，第一和第二电极彼此接触以导致传导。 第一和第二电极连接到控制装置，并且当第一和第二电极进入导通状态时，控制装置打开控制阀。 由于通过这种方式通过按压力打开和关闭控制阀，所以可以容易地供应燃料溶液。

公开(公告)号：US08911909B2

公开(公告)日：2014-12-16

申请号：US13699061

申请日：2011-04-25

申请人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

发明人： Daisuke Yamaguchi ,  Tsunetoshi Samukawa ,  Shuji Fujita ,  Hiroki Mita ,  Takaaki Nakagawa ,  Yuichi Tokita

IPC分类号： H01M8/16 ,  H01M8/04 ,  H01M8/24

CPC分类号： H01M8/04201 ,  H01M8/04082 ,  H01M8/16 ,  H01M8/2455 ,  Y02E60/527

摘要： The valve control unit of a fuel leakage preventing structure of the present invention includes first and second electrodes. The first electrode is attached to a first member that is movable. When a pressing force is applied to the first member to move, the first and second electrodes are brought into contact with each other, to cause conduction. The first and second electrodes are connected to a control device, and the control device opens a control valve when the first and second electrodes are put into a conducting state. As the control valve is opened and closed by a pressing force in this manner, a fuel solution can be easily supplied.

摘要翻译： 本发明的燃料泄漏防止结构的阀门控制单元包括第一和第二电极。 第一电极附接到可移动的第一构件。 当对第一构件施加按压力以移动时，第一和第二电极彼此接触以导致传导。 第一和第二电极连接到控制装置，并且当第一和第二电极进入导通状态时，控制装置打开控制阀。 由于通过这种方式通过按压力打开和关闭控制阀，所以可以容易地供应燃料溶液。

公开(公告)号：US20140024102A1

公开(公告)日：2014-01-23

申请号：US13985508

申请日：2012-02-24

申请人： Hideki Sakai ,  Ryuhei Matsumoto ,  Shuji Fujita ,  Yoshio Goto ,  Yuichi Tokita

发明人： Hideki Sakai ,  Ryuhei Matsumoto ,  Shuji Fujita ,  Yoshio Goto ,  Yuichi Tokita

IPC分类号： H01M8/16 ,  C12N15/63

CPC分类号： H01M8/16 ,  C12N9/0006 ,  C12N15/63 ,  Y02E60/527

摘要： As a technique for increasing the output of a microbial fuel cell, a microbial fuel cell including a polyol such as glycerol as a fuel and using a microbe in which an enzyme that catalyzes a redox reaction has been introduced by genetic recombination on the side of a negative electrode is provided. By this microbial fuel cell, the velocity of the reaction can be increased to thereby give a high output by retaining a microbe in which an enzyme that catalyzes a redox reaction such as diaphorase has been introduced by genetic recombination on the side of the negative electrode.

摘要翻译： 作为增加微生物燃料电池的输出的技术，包括多元醇如甘油作为燃料并使用其中催化氧化还原反应的酶的微生物的微生物燃料电池已通过基因重组引入到 提供负电极。 通过这种微生物燃料电池，可以增加反应的速度，从而通过保留在负极侧通过遗传重组引入催化氧化还原反应的酶如氧化黄酶的微生物而产生高产量。

公开(公告)号：US20110136022A1

公开(公告)日：2011-06-09

申请号：US13058107

申请日：2010-06-04

申请人： Ryuhei Matsumoto ,  Hideki Sakai ,  Yuichi Tokita ,  Shuji Fujita

发明人： Ryuhei Matsumoto ,  Hideki Sakai ,  Yuichi Tokita ,  Shuji Fujita

IPC分类号： H01M8/16 ,  H01M8/00 ,  H01M4/90 ,  G01N33/50 ,  G01N27/327

CPC分类号： H01M8/16 ,  C12N11/04 ,  H01M4/8825 ,  H01M4/90 ,  H01M8/0625 ,  Y02E60/527 ,  Y02P70/56

摘要： There are provided a fuel cell and a production method therefor in which one or more types of enzymes or further coenzymes are enclosed in a micro space so that electrons can be efficiently extracted from a fuel such as glucose or the like by an enzyme reaction using the micro space as a reaction field, thereby producing electric energy, and in which the enzyme or further the coenzyme can be easily immobilized on an electrode.Enzymes 13 and 14 and a coenzyme 15 necessary for an enzyme reaction are enclosed in liposome 12, and the liposome 12 is immobilized on a surface of an electrode composed of porous carbon or the like to form an enzyme-immobilized electrode. An antibiotic 16 is bonded to a bimolecular lipid membrane constituting the liposome 12 to form one or more pores 17 permeable to glucose. The enzyme-immobilized electrode is used as, for example, a negative electrode of a biofuel cell.

摘要翻译： 提供了一种燃料电池及其制造方法，其中将一种或多种类型的酶或其它辅酶封闭在微空间中，使得可以通过酶反应从诸如葡萄糖等的燃料有效地提取电子，使用 微空间作为反应场，从而产生电能，并且其中酶或其它辅酶可以容易地固定在电极上。 将酶13和14以及酶反应所必需的辅酶15包封在脂质体12中，将脂质体12固定在由多孔碳等构成的电极的表面上，形成酶固定化电极。 抗生素16与构成脂质体12的双分子脂质膜结合形成一个或多个可透过葡萄糖的孔17。 酶固定化电极用作例如生物燃料电池的负极。

公开(公告)号：US20140065494A1

公开(公告)日：2014-03-06

申请号：US13982099

申请日：2012-01-24

申请人： Shuji Fujita ,  Hiroki Mita ,  Shun Yamanoi ,  Hideki Sakai ,  Yuichi Tokita ,  Seiichiro Tabata ,  Tsunetoshi Samukawa ,  Takaaki Nakagawa

发明人： Shuji Fujita ,  Hiroki Mita ,  Shun Yamanoi ,  Hideki Sakai ,  Yuichi Tokita ,  Seiichiro Tabata ,  Tsunetoshi Samukawa ,  Takaaki Nakagawa

IPC分类号： H01M4/90 ,  C25B11/04 ,  G01N27/327

CPC分类号： H01M4/9008 ,  C25B11/0447 ,  G01N27/327 ,  H01M4/9083 ,  H01M8/16 ,  Y02E60/527 ,  Y02P70/56

摘要： Provided is a fuel cell capable of preventing elution of nicotinamide adenine dinucleotide and/or a derivative thereof immobilized on an electrode, and capable of preventing performance degradation due to elution, and a method for manufacturing the fuel cell.A biofuel cell having a structure in which a positive electrode and a negative electrode face each other via a proton conductor, the biofuel cell configured so that an enzyme is used to extract electrons from a fuel, wherein the negative electrode is configured from an electrode including carbon and/or an inorganic compound having pores with a size of 2 nm or more and 100 nm or less on the surface, nicotinamide adenine dinucleotide and/or a derivative thereof being immobilized on the carbon and/or the inorganic compound. A carbon particle, a carbon sheet, or carbon fiber is used as the carbon.

摘要翻译： 提供一种燃料电池，其能够防止固定在电极上的烟酰胺腺嘌呤二核苷酸和/或其衍生物的洗脱，并且能够防止由于洗脱引起的性能劣化，以及燃料电池的制造方法。 一种生物燃料电池，其具有通过质子导体将正极和负极面对的结构，所述生物燃料电池被配置为使得酶用于从燃料中提取电子，其中所述负极由电极构成，所述电极包括 碳和/或具有尺寸为2nm以上且100nm以下的孔的无机化合物，烟酰胺腺嘌呤二核苷酸和/或其衍生物固定在碳和/或无机化合物上。 使用碳粒子，碳片或碳纤维作为碳。

公开(公告)号：US20110250511A1

公开(公告)日：2011-10-13

申请号：US13078552

申请日：2011-04-01

申请人： Tsunetoshi Samukawa ,  Taiki Sugiyama ,  Shuji Fujita ,  Yuichi Tokita ,  Hideki Sakai

发明人： Tsunetoshi Samukawa ,  Taiki Sugiyama ,  Shuji Fujita ,  Yuichi Tokita ,  Hideki Sakai

IPC分类号： H01M8/16

CPC分类号： H01M8/16 ,  H01M4/8657 ,  H01M8/04186 ,  H01M8/08 ,  H01M8/1009 ,  Y02E60/527

摘要： Disclosed herein is a biofuel cell including a polymer gel reversibly swelling and contracting in response to variations in a property of a fuel solution making contact therewith, the polymer gel being on a surface of an electrode and/or in the inside of the electrode.

摘要翻译： 本文公开了一种生物燃料电池，其包括聚合物凝胶，其响应于与其接触的燃料溶液的性质的变化而可逆地膨胀和收缩，所述聚合物凝胶位于电极的表面和/或电极的内部。

公开(公告)号：US20100121530A1

公开(公告)日：2010-05-13

申请号：US12522603

申请日：2007-12-28

申请人： Yoshiaki Suzuki ,  Shuji Fujita

发明人： Yoshiaki Suzuki ,  Shuji Fujita

IPC分类号： G06F19/00 ,  B62D5/04

CPC分类号： B62D5/0457 ,  B62D5/0475 ,  B62D5/0481

摘要： When the magnitude |ω| of a steering angular velocity ω is equal to or greater than a determination angular velocity ωth, an electric motor is estimated to be in a predetermined power use state, and a restriction determination threshold Vonth is set to a first voltage value V1 (S24). When the magnitude |ω| of the steering angular velocity ω is less than the determination angular velocity ωth, the restriction determination threshold Vonth is set to a second voltage value V2 (>V1) (S25). When a state in which a power supply voltage Vx is lower than the restriction determination threshold Vonth continues for a predetermined time t1 (S30: Yes), a power use restriction instruction is output to specific vehicle electrical loads. Thus, power restriction is properly imposed on the vehicle electrical loads, whereby a delay in control of the electric motor is prevented, and excessive imposition of power restriction is prevented.

摘要翻译： 当幅度|ω|时 转向角速度ω等于或大于确定角速度ωth，则估计电动机处于预定功率使用状态，并将限制判定阈值Vonth设置为第一电压值V1（S24）。 当幅度|ω|时 转向角速度ω小于确定角速度ωth，将限制判定阈值Vonth设定为第二电压值V2（> V1）（S25）。 当电源电压Vx低于限制判定阈值Vonth的状态持续预定时间t1（S30：是）时，将电力使用限制指令输出到特定的车辆电气负载。 因此，对车辆电气负载适当地施加功率限制，由此防止电动机的控制延迟，并且防止了过度的功率限制。