公开(公告)号：US09834806B2

公开(公告)日：2017-12-05

申请号：US13001465

申请日：2009-06-25

申请人： Hideyuki Noda ,  Masahiro Okanojo ,  Kenko Uchida ,  Norihito Kuno

发明人： Hideyuki Noda ,  Masahiro Okanojo ,  Kenko Uchida ,  Norihito Kuno

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12Q1/06 ,  C12Q1/00 ,  B01L3/00 ,  G01N1/22

CPC分类号： C12Q1/008 ,  B01L3/502 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/1805 ,  B01L2400/0487 ,  G01N1/2208 ,  G01N1/2273

摘要： A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped. The contact with the warm water causes the carrier to be solated filtered through the filter.

公开(公告)号：US20110183371A1

公开(公告)日：2011-07-28

申请号：US13001465

申请日：2009-06-25

申请人： Hideyuki Noda ,  Masahiro Okanojo ,  Kenko Uchida ,  Norihito Kuno

发明人： Hideyuki Noda ,  Masahiro Okanojo ,  Kenko Uchida ,  Norihito Kuno

IPC分类号： C12Q1/06 ,  C12M1/00 ,  C12M1/34

CPC分类号： C12Q1/008 ,  B01L3/502 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/1805 ,  B01L2400/0487 ,  G01N1/2208 ,  G01N1/2273

摘要： A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, such as gelatin, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped container and is stored in the liquid storage sink, thereby causing the carrier to make contact with the warm water. The contact with the warm water causes the carrier to be solated, and mixed and diluted with the warm water. Then, the suction pump of the filtration device is activated for suction and filtration of the mixed liquid through the filter. By using the filter having a shape not allowing microbes to pass through and having a gap, only microbes are captured on the filter after suction and filtration. Polymers and dust to be a background upon an ATP measurement pass through the filter by filtration to be cleanly eliminated.

摘要翻译： 提供一种一次性微生物收集载体盒和载体处理装置，其将收集在载体上的微生物有效地转化为溶液并防止在过滤器上浓缩时微生物的回收量的变化。 由具有多个通孔的上盖形成的盒，具有倒置锥形的容器，其具有在底部具有过滤器的液体储存槽单元和空气吸入路径，以及用于设置 制备诸如明胶的热塑性载体，以及由用于液体供应的分配喷嘴形成的载体处理装置，液体供给机构，液体加热机构和用于过滤的抽吸泵。 向设置在倒锥形容器的底面上的过滤器供给温水，并将其储存在液体储存槽中，从而使载体与温水接触。 与温水接触导致载体被溶解，并与温水混合并稀释。 然后，过滤装置的抽吸泵被激活，以通过过滤器抽吸和过滤混合液体。 通过使用具有不允许微生物通过并具有间隙的形状的过滤器，在抽吸和过滤之后仅在过滤器上捕获微生物。 聚合物和粉尘作为ATP测量的背景，通过过滤通过过滤器，以便清洁地消除。

公开(公告)号：US08334144B2

公开(公告)日：2012-12-18

申请号：US13478527

申请日：2012-05-23

申请人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76 ,  G01N21/00 ,  G01N21/75

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光箱，其具有样本容器保持器和其中的快门单元，所述快门单元包括部分地由板构件的运动形成的顶板和在其中具有光电检测器的第二遮光箱。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

公开(公告)号：US20080261294A1

公开(公告)日：2008-10-23

申请号：US12034880

申请日：2008-02-21

申请人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76 ,  C12M1/36

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光盒，其具有样品容器保持器和其中的快门单元，所述快门单元包括顶板，该顶板部分地通过板构件的运动和在其中具有光电检测器的第二遮光箱而形成。 虽然没有实现测量，但是关闭快门单元以阻止杂散光入射到光电检测器，并且在实施测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入通孔 使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

公开(公告)号：US08197751B2

公开(公告)日：2012-06-12

申请号：US12976031

申请日：2010-12-22

申请人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N21/00 ,  G01N21/75

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

公开(公告)号：US20110091355A1

公开(公告)日：2011-04-21

申请号：US12976031

申请日：2010-12-22

申请人： Hideyuki NODA ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki NODA ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member, and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光箱，其具有样本容器保持器和其中的快门单元，所述快门单元包括部分地由板构件的运动形成的顶板和在其中具有光电检测器的第二遮光箱。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

公开(公告)号：US07879290B2

公开(公告)日：2011-02-01

申请号：US12034880

申请日：2008-02-21

申请人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

发明人： Hideyuki Noda ,  Satoshi Ozawa ,  Masahiro Okanojo ,  Kenko Uchida

IPC分类号： G01N21/76 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N21/00 ,  G01N21/75

CPC分类号： C12Q1/04 ,  G01N21/76

摘要： An apparatus includes a system for guiding chemiluminescence and a system for preventing a variation in dark currents. The apparatus includes a first light shielding BOX having a sample container holder and a shutter unit therein, the shutter unit including a top plate which is partly formed by a movement of a plate member and a second light shielding BOX having a photodetector therein. While a measurement is not implemented, the shutter unit is closed to block entrance of stray light to the photodetector, and while a measurement is implemented, the plate member is moved to open the shutter unit, and the tip of the photodetector is inserted into a through hole formed in the top plate, so that the distance between the bottom of the sample container and a sensitive area of the photodetector is reduced to several millimeters or less.

摘要翻译： 一种装置包括用于引导化学发光的系统和用于防止暗电流变化的系统。 该装置包括：第一遮光盒，其具有样品容器保持器和其中的快门单元，所述快门单元包括顶板，该顶板部分地通过板构件的运动和在其中具有光电检测器的第二遮光箱而形成。 虽然没有实现测量，但是快门单元被关闭以阻止杂散光入射到光电检测器，并且在实现测量的同时，移动板构件以打开快门单元，并且将光电检测器的末端插入到 通孔形成在顶板中，使得样品容器的底部与光电检测器的敏感区域之间的距离减小到几毫米或更小。

公开(公告)号：US07737268B2

公开(公告)日：2010-06-15

申请号：US10848368

申请日：2004-05-19

申请人： Norihito Kuno ,  Kenko Uchida

发明人： Norihito Kuno ,  Kenko Uchida

IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/1006

摘要： Disclosure is a method of separately recovering deoxyribonucleic acid and ribonucleic acid from a biological sample solution. They are separated and recovered from a single biological sample by controlling pH values, temperature, and/or concentration of cationic ions of a nucleic acid sample solution containing a caotropic agent. It is possible to easily and safely separate and recover deoxyribonucleic acid and ribonucleic acid from the single biological sample.

摘要翻译： 披露是从生物样品溶液中分离回收脱氧核糖核酸和核糖核酸的方法。 通过控制含有约束剂的核酸样品溶液的阳离子离子的pH值，温度和/或浓度，从单个生物样品中分离和回收它们。 可以从单一生物样品中容易且安全地分离和回收脱氧核糖核酸和核糖核酸。

公开(公告)号：US20090111114A1

公开(公告)日：2009-04-30

申请号：US12251375

申请日：2008-10-14

申请人： Yoshihiro Yamashita ,  Toshinari Sakurai ,  Norihito Kuno ,  Kenko Uchida ,  Toshiaki Yokobayashi

发明人： Yoshihiro Yamashita ,  Toshinari Sakurai ,  Norihito Kuno ,  Kenko Uchida ,  Toshiaki Yokobayashi

IPC分类号： C12Q1/68

CPC分类号： C12N15/1006 ,  C12N15/101

摘要： A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.

摘要翻译： 提供了一种以安全，快速，简单的方法从含有RNA的生物材料中提取高纯度RNA的方法和分析方法。 该方法包括以下步骤：将含有预定浓度的离液剂和预定浓度的有机溶剂的含有RNA的生物材料混合，使混合溶液与核酸结合固相接触，洗涤核酸结合固体 RNA结合的RNA，并从具有结合RNA的核酸结合固相中洗脱RNA。 此外，通过逆转录酶 - 聚合酶链反应（RT-PCR）等分析得到的RNA。

公开(公告)号：US20050123965A1

公开(公告)日：2005-06-09

申请号：US10981521

申请日：2004-11-05

申请人： Yoshihiro Yamashita ,  Toshinari Sakurai ,  Norihito Kuno ,  Kenko Uchida ,  Toshiaki Yokobayashi

发明人： Yoshihiro Yamashita ,  Toshinari Sakurai ,  Norihito Kuno ,  Kenko Uchida ,  Toshiaki Yokobayashi

IPC分类号： C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/1006 ,  C12N15/101

摘要： A method to extract RNA with high purity from biological materials containing RNA in a safe, rapid, and simple procedure and a method to analyze it are provided. The procedure includes the steps of mixing a biological material containing RNA with a predetermined concentration of a chaotropic agent and a predetermined concentration of an organic solvent, allowing the mixed solution to contact a nucleic acid-binding solid phase, washing the nucleic-acid binding solid-phase to which RNA is bound, and eluting RNA from the nucleic-acid binding solid-phase having the bound RNA. Furthermore, the obtained RNA is analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) or the like.

摘要翻译： 提供了一种以安全，快速，简单的方法从含有RNA的生物材料中提取高纯度RNA的方法和分析方法。 该方法包括以下步骤：将含有预定浓度的离液剂和预定浓度的有机溶剂的含有RNA的生物材料混合，使混合溶液与核酸结合固相接触，洗涤核酸结合固体 RNA结合的RNA，并从具有结合RNA的核酸结合固相中洗脱RNA。 此外，通过逆转录酶 - 聚合酶链反应（RT-PCR）等分析得到的RNA。