公开(公告)号：US20050032201A1

公开(公告)日：2005-02-10

申请号：US10936937

申请日：2004-09-09

申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  C12M1/34

CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are present in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的存在量，即使从固相中回收的细胞与固相相结合， 聚合物，从液相中回收的细胞不与聚合物结合固相。

公开(公告)号：US06926915B1

公开(公告)日：2005-08-09

申请号：US09937510

申请日：2000-03-30

申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  A61K35/78

CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的量使得尽管从固相中回收的细胞与固相结合， 从液相中回收的细胞不与聚合物结合固相。

公开(公告)号：US20050214758A1

公开(公告)日：2005-09-29

申请号：US10498579

申请日：2002-12-09

申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa

发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa

IPC分类号： G01N33/537 ,  G01N33/569 ,  G01N33/68 ,  C12Q1/68 ,  C12M1/34 ,  G01N33/567

CPC分类号： G01N33/689 ,  G01N33/537 ,  G01N33/56966 ,  G01N2400/00 ,  G01N2800/368

摘要： A blood cell separating system is offered for precisely separating and concentrating rare fetal nucleated cells intermixed in the blood of a pregnant woman, to conveniently obtain test preparations capable of being used for prenatal chromosomal/genetic diagnosis. A blood cell separating system is characterized by comprising (1) a primary separating device for removing mainly non-nucleated erythrocytes, leukocytes and platelets from blood samples taken from a pregnant woman to obtain a primary separated sample, (2) a secondary separating device for using a carbohydrate-lectin method to remove residual non-nucleated erythrocytes and leukocytes from the primary separated sample obtained by the primary separating device to obtain a secondary separated sample with concentrated fetal nucleated cells, and (3) preparing device for preparing the secondary separated sample obtained by the secondary separating device.

摘要翻译： 提供血细胞分离系统，用于精确分离和浓缩在孕妇血液中混合的稀有胎儿有核细胞，以方便地获得能够用于产前染色体/遗传学诊断的测试制剂。 血细胞分离系统的特征在于包括：（1）主要分离装置，用于从孕妇取血的样品中主要去除未成核的红细胞，白细胞和血小板，以获得初级分离的样品;（2）二次分离装置， 使用碳水化合物凝集素方法从初级分离装置获得的主要分离样品中除去残留的未成核红细胞和白细胞，以获得具有浓缩胎儿有核细胞的二次分离样品，和（3）制备二次分离样品的制备装置 通过二次分离装置获得。