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    Method for selectively separating blood cells by using lectin
    1.
    发明申请
    Method for selectively separating blood cells by using lectin 审中-公开
    通过使用凝集素选择性分离血细胞的方法

    公开(公告)号：US20050032201A1

    公开(公告)日：2005-02-10

    申请号：US10936937

    申请日：2004-09-09

    申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  C12M1/34

    CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

    摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are present in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

    摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的存在量，即使从固相中回收的细胞与固相相结合， 聚合物，从液相中回收的细胞不与聚合物结合固相。

    Method for selectively separating blood cells by using lectin
    2.
    发明授权
    Method for selectively separating blood cells by using lectin 有权
    通过使用凝集素选择性分离血细胞的方法

    公开(公告)号：US06926915B1

    公开(公告)日：2005-08-09

    申请号：US09937510

    申请日：2000-03-30

    申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  A61K35/78

    CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

    摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

    摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的量使得尽管从固相中回收的细胞与固相结合， 从液相中回收的细胞不与聚合物结合固相。

    Blood cell separating system
    3.
    发明申请
    Blood cell separating system 审中-公开
    血细胞分离系统

    公开(公告)号：US20050214758A1

    公开(公告)日：2005-09-29

    申请号：US10498579

    申请日：2002-12-09

    申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa

    发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa

    IPC分类号： G01N33/537 ,  G01N33/569 ,  G01N33/68 ,  C12Q1/68 ,  C12M1/34 ,  G01N33/567

    CPC分类号： G01N33/689 ,  G01N33/537 ,  G01N33/56966 ,  G01N2400/00 ,  G01N2800/368

    摘要： A blood cell separating system is offered for precisely separating and concentrating rare fetal nucleated cells intermixed in the blood of a pregnant woman, to conveniently obtain test preparations capable of being used for prenatal chromosomal/genetic diagnosis. A blood cell separating system is characterized by comprising (1) a primary separating device for removing mainly non-nucleated erythrocytes, leukocytes and platelets from blood samples taken from a pregnant woman to obtain a primary separated sample, (2) a secondary separating device for using a carbohydrate-lectin method to remove residual non-nucleated erythrocytes and leukocytes from the primary separated sample obtained by the primary separating device to obtain a secondary separated sample with concentrated fetal nucleated cells, and (3) preparing device for preparing the secondary separated sample obtained by the secondary separating device.

    摘要翻译： 提供血细胞分离系统，用于精确分离和浓缩在孕妇血液中混合的稀有胎儿有核细胞，以方便地获得能够用于产前染色体/遗传学诊断的测试制剂。 血细胞分离系统的特征在于包括：（1）主要分离装置，用于从孕妇取血的样品中主要去除未成核的红细胞，白细胞和血小板，以获得初级分离的样品;（2）二次分离装置， 使用碳水化合物凝集素方法从初级分离装置获得的主要分离样品中除去残留的未成核红细胞和白细胞，以获得具有浓缩胎儿有核细胞的二次分离样品，和（3）制备二次分离样品的制备装置 通过二次分离装置获得。

    Functional chitosan derivative
    5.
    发明授权
    Functional chitosan derivative 有权
    功能性壳聚糖衍生物

    公开(公告)号：US07125968B2

    公开(公告)日：2006-10-24

    申请号：US10819490

    申请日：2004-04-07

    申请人： Hirofumi Yura ,  Yoshio Saito ,  Masayuki Ishihara ,  Katsuaki Ono ,  Shiro Saeki

    发明人： Hirofumi Yura ,  Yoshio Saito ,  Masayuki Ishihara ,  Katsuaki Ono ,  Shiro Saeki

    IPC分类号： C08B37/08 ,  C07H5/04

    CPC分类号： A61Q19/00 ,  A61K8/736 ,  A61K2800/57 ,  A61L15/28 ,  A61L33/08 ,  A61Q5/00 ,  A61Q17/04 ,  A61Q19/08 ,  C08B37/003 ,  C08L5/08

    摘要： A functional chitosan derivative which comprises a chitin/chitosan, which is a natural polysaccharide, and incorporated therein at least one of a carbohydrate, a photo-reactive functional group, an amphipathic group, e.g. a polyoxyethylene alkyl ether, and a glycosaminoglycan and which, due to the incorporation, has solubility in a neutral medium, self-crosslinking ability, the property of highly containing water or healing wounds, and antithrombotic properties. Namely, the derivative has various properties required of health care materials such as medical products and cosmetics.

    摘要翻译： 一种功能性壳聚糖衍生物，其包含几丁质/壳聚糖，其为天然多糖，并且其中并入至少一种碳水化合物，光反应性官能团，两亲性基团等。 聚氧乙烯烷基醚和糖胺聚糖，由于其结合，在中性介质中具有溶解性，自交联能力，高含水或愈合伤口的性质以及抗血栓性质。 即，该衍生物具有医疗用品，化妆品等保健品所需的各种特性。

    Functionalized glycosaminoglycan polymer and medical instruments and drugs by using the same
    6.
    发明授权
    Functionalized glycosaminoglycan polymer and medical instruments and drugs by using the same 有权
    官能化的糖胺聚糖和医疗仪器和药物使用相同

    公开(公告)号：US07005513B1

    公开(公告)日：2006-02-28

    申请号：US09937991

    申请日：2000-03-30

    申请人： Hirofumi Yura ,  Yoshio Saito ,  Masayuki Ishihara ,  Katsuaki Ono ,  Keiichi Ishikawa

    发明人： Hirofumi Yura ,  Yoshio Saito ,  Masayuki Ishihara ,  Katsuaki Ono ,  Keiichi Ishikawa

    IPC分类号： C08B37/00 ,  C07H7/00

    CPC分类号： C08F246/00 ,  C08B37/0063

    摘要： The present invention provides a functionalized polymer which can be used extensively in the field of medical drugs as well as medical devices and which is obtainable in an organic synthetic manner from glycosaminoglycan controlling adhesion, migration and proliferation of cells via linkage to various cellular growth factors or cytokines or direct interactions with the cells. The functionalized polymer of the present invention is characterized in that it comprises a carbohydrate corresponding to at least a part of the basic structure of glycosaminoglycan introduced into a vinyl-type polymer chain.

    摘要翻译： 本发明提供了可广泛用于医疗药物以及医疗器械领域的官能化聚合物，其可以通过与各种细胞生长因子的连接控制细胞粘附，迁移和增殖的糖胺聚糖以有机合成方式获得，或 细胞因子或与细胞的直接相互作用。 本发明的官能化聚合物的特征在于，其包含对应于导入乙烯基型聚合物链的糖胺聚糖的至少一部分碱性结构的碳水化合物。

    Optical pickup
    8.
    再颁专利
    Optical pickup 有权

    公开(公告)号：USRE40928E1

    公开(公告)日：2009-10-06

    申请号：US11645328

    申请日：2006-12-26

    申请人： Noriyuki Kawano ,  Kouichi Ogura ,  Yoshio Saito ,  Mitsuru Kinouchi

    发明人： Noriyuki Kawano ,  Kouichi Ogura ,  Yoshio Saito ,  Mitsuru Kinouchi

    IPC分类号： G11B7/00

    CPC分类号： G11B7/0932 ,  G11B7/093 ,  G11B7/0933 ,  G11B7/0935 ,  G11B7/0956 ,  G11B7/22

    摘要： An optical pickup comprises an objective-lens driving device including a movable section provided with an objective lens, a focusing coil, and tracking coils, and a fixed section provided with a single magnetic circuit having a magnetic gap, the focusing coil and the tracking coils being disposed in the magnetic gap. The point of application of a tracking-driving force, the point of application of a result force of focusing-driving forces occurring in and outside the magnetic gap, and the position of the center of gravity of the movable section are made to substantially coincide with each other. In addition, the objective lens and the magnetic circuit are disposed within an area of a window in a lower shell of a optical disk. Further, a through hole for accommodating a lower portion of a yoke of the magnetic circuit is provided in a mounting base on which the objective-lens driving device is mounted. An inclining fulcrum and a height adjusting means for inclining the objective-lens driving device about the inclining fulcrum are provided in the vicinities of the through hole.