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1.发明授权Protein substance having triple helix structure and manufacturing method therefor 有权具有三螺旋结构的蛋白质物质及其制造方法公开(公告)号：US08470555B2
公开(公告)日：2013-06-25
申请号：US13141138
申请日：2009-12-22
CPC分类号： C07K14/78 , C07K14/4726 , C07K2319/00 , C07K2319/02
摘要： As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要翻译： 作为专门研究的结果，本发明人已经成功地发明了一种胶原基因构建体，其可以容易地纯化并保持与天然存在的胶原蛋白相当的三重螺旋结构，同时具有低分子量。 具体地，通过亲和纯化进行一步纯化，因为CR-D（信号肽）具有碳水化合物识别结构域。 通过将本发明的人胶原结构基因的一部分用MBL的胶原样结构基因部分代替，可以以高纯度获得维持三螺旋结构并且是热稳定的低分子量胶原， 大批量。
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2.发明授权Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof 有权用于使用动物细胞大量生产来自外来基因的蛋白质的表达载体及其用途公开(公告)号：US09096878B2
公开(公告)日：2015-08-04
申请号：US13140905
申请日：2009-12-22
CPC分类号： C12P21/02 , C12N15/85 , C12N2830/00
摘要： The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人进行了专门研究并成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的二氢叶酸还原酶基因顺反子，其表达已通过将密码子改变为最少 常用的哺乳动物密码子 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
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3.发明申请EXPRESSION VECTOR FOR PRODUCING PROTEIN DERIVED FROM FOREIGN GENE IN LARGE QUANTITY USING ANIMAL CELLS, AND USE THEREOF 有权用动物细胞生产大量外源基因衍生蛋白的表达载体及其用途公开(公告)号：US20120122083A1
公开(公告)日：2012-05-17
申请号：US13140905
申请日：2009-12-22
CPC分类号： C12P21/02 , C12N15/85 , C12N2830/00
摘要： The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人进行了专门研究并成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的二氢叶酸还原酶基因顺反子，其表达已通过将密码子改变为最少 常用的哺乳动物密码子 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
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4.发明申请PROTEIN SUBSTANCE HAVING TRIPLE HELIX STRUCTURE AND MANUFACTURING METHOD THEREFOR 有权具有三重螺旋结构的蛋白质物质及其制造方法公开(公告)号：US20120059152A1
公开(公告)日：2012-03-08
申请号：US13141138
申请日：2009-12-22
CPC分类号： C07K14/78 , C07K14/4726 , C07K2319/00 , C07K2319/02
摘要： As a result of dedicated research, the present inventors have successfully invented a collagen gene construct which can be easily purified and maintains a triple helix structure equivalent to that of naturally-occurring collagen while having a low molecular weight. Specifically, one-step purification by affinity purification is enabled because CR-D (a signal peptide) has a carbohydrate recognition domain. By substituting a portion of a human collagen structural gene of the present invention with the collagen-like structural gene portion of MBL, a low-molecular-weight collagen which maintains a triple helix structure and is thermally stable can be obtained with high purity and in large quantities.
摘要翻译： 作为专门研究的结果，本发明人已经成功地发明了一种胶原基因构建体，其可以容易地纯化并保持与天然存在的胶原蛋白相当的三重螺旋结构，同时具有低分子量。 具体地，通过亲和纯化进行一步纯化，因为CR-D（信号肽）具有碳水化合物识别结构域。 通过将本发明的人胶原结构基因的一部分用MBL的胶原样结构基因部分代替，可以以高纯度获得维持三螺旋结构并且是热稳定的低分子量胶原， 大批量。
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5.发明授权Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof 有权用于使用动物细胞大规模生产外源基因衍生蛋白质的表达载体及其用途公开(公告)号：US08653249B2
公开(公告)日：2014-02-18
申请号：US12918776
申请日：2009-02-27
CPC分类号： C12N15/85 , C12N9/1205 , C12N2830/50 , C12N2840/102 , C12Y207/01095
摘要： The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的药物抗性基因顺反子，其表达已通过将密码子改变为最不常用的密码子而被减弱 哺乳动物 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
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6.发明申请EXPRESSION VECTOR FOR MASS PRODUCTION OF FOREIGN GENE-DERIVED PROTEIN USING ANIMAL CELL AND USE THEREOF 有权使用动物细胞大量生产外源基因衍生蛋白的表达载体及其用途公开(公告)号：US20110123993A1
公开(公告)日：2011-05-26
申请号：US12918776
申请日：2009-02-27
CPC分类号： C12N15/85 , C12N9/1205 , C12N2830/50 , C12N2840/102 , C12Y207/01095
摘要： The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
摘要翻译： 本发明人成功构建了能够在哺乳动物宿主细胞中高水平生产外源基因衍生蛋白质的表达载体，其包含翻译受损的药物抗性基因顺反子，其表达已通过将密码子改变为最不常用的密码子而被减弱 哺乳动物 以及具有用于在高转录活性启动子和高度稳定的聚腺苷酸化信号之间引入外源基因的克隆位点的基因盒。
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公开(公告)号：US5596243A
公开(公告)日：1997-01-21
申请号：US318336
申请日：1994-10-05
CPC分类号： H03H9/1035 , H03H9/0595
摘要： The invention is a crystal oscillator having a vibrating plate comprising a vibrating part surrounded by a rim part spaced from the vibrating part except for a narrow connecting part that connects the vibrating part to the rim part in a cantilever fashion. The thickness of the vibrating part and the connecting part are less than the thickness of the rim part. The vibrating part is sandwiched between a pair of casings. Each of the casings has a recess facing the vibrating part, which prevent the vibrating part from contacting the casings. Each surface facing a recess has an electrode positioned thereon and an electrode lead extending from the surface electrode to the connecting part. Each casing has a through-hole positioned to face an electrical lead. A cup-shaped metal lead is placed in each through-hole with the bottom of the cup contacting the electrical lead facing the through-hole. Current to the surface electrodes is supplied through the metal leads.
摘要翻译： 本发明是一种具有振动板的晶体振荡器，该振动板包括由与振动部分隔开的边缘部分包围的振动部分，除了以悬臂方式将振动部分连接到轮辋部分的窄连接部分。 振动部分和连接部分的厚度小于边缘部分的厚度。 振动部分夹在一对外壳之间。 每个壳体具有面向振动部的凹部，其防止振动部与壳体接触。 面向凹部的每个表面具有位于其上的电极和从表面电极延伸到连接部分的电极引线。 每个壳体具有定位成面对电引线的通孔。 杯形金属引线放置在每个通孔中，杯的底部接触电引线面向通孔。 通过金属引线供给到表面电极的电流。
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8.发明授权Process for processing a waste etching solution containing a fluorine component and an ammonia component to recover valuables therefrom 失效用于处理含有氟组分和氨组分的废蚀刻溶液以从其中回收贵重物质的方法
公开(公告)号：US5281318A
公开(公告)日：1994-01-25
申请号：US918706
申请日：1992-07-27
申请人： Hiroshi Tahara
发明人： Hiroshi Tahara
CPC分类号： B01D61/44 , B01D61/445
摘要： A process for recovering valuables from a waste etching solution containing a fluorine component and ammonia component is provided comprising recovering respectively a fluorine component and ammonia component in the waste solution and recovering a potassium and/or sodium component used for processing the waste solution, and utilizing respectively the recovered components for preparation of an etching solution or reusing or recycling for processing the waste solution. That is, this process comprises adding at least one of potassium hydroxide and sodium hydroxide to the waste etching solution containing a fluorine component and an ammonia component to form hydroxides of metal ions dissolved in the waste solution, filtering the neutralized solution for separation of the metal hydroxides, heating the filtrate by steam injection to recover the generated ammonia as ammonia gas, aqueous ammonia or liquid ammonia, then subjecting the aqueous solution (KF and/or NaF solution free from ammonia components) after recovering the ammonia component to processing using a monopolar or bipolar membrane to recover the fluorine component as an aqueous hydrofluoric acid solution, further recovering at least one of the potassium component and sodium component as at least one of potassium hydroxide and sodium hydroxide and recycling and reusing the recovered ammonia and recovered aqueous hydrofluoric acid solution for the preparation of a new etching solution and at least one of the recovered potassium hydroxide and sodium hydroxide for processing the waste etching solution as a neutralizing agent.
摘要翻译： 提供了从含有氟组分和氨组分的废蚀刻溶液中回收贵重物品的方法，包括分别回收废溶液中的氟组分和氨组分并回收用于处理废溶液的钾和/或钠组分，并利用 分别用于制备蚀刻溶液的回收组分或用于处理废溶液的再利用或再循环。 也就是说，该方法包括向含有氟组分和氨组分的废蚀刻溶液中加入氢氧化钾和氢氧化钠中的至少一种，以形成溶解在废溶液中的金属离子的氢氧化物，过滤中和的溶液以分离金属 氢氧化物，通过蒸汽喷射加热滤液以回收产生的氨作为氨气，氨水或液氨，然后在将氨组分回收到使用单极的处理之后，使水溶液（KF和/或NaF溶液不含氨组分） 或双极膜以氟代酸水溶液回收氟组分，进一步回收至少一种钾组分和钠组分作为氢氧化钾和氢氧化钠中的至少一种，并循环利用回收的氨和回收的氢氟酸水溶液 用于制备新的蚀刻解决方案和在leas 一种回收的氢氧化钾和氢氧化钠，用于处理废蚀刻溶液作为中和剂。
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公开(公告)号：US4517546A
公开(公告)日：1985-05-14
申请号：US515228
申请日：1983-07-19
CPC分类号： G06F3/045 , G06K9/24 , Y10T29/49099 , Y10T29/49124
摘要： A resistor sheet input tablet comprising two rectangular resistor sheets each provided at two opposite edges thereof with electrodes, the rectangular resistor sheets being superposed such that the electrodes on one of the resistor sheets lie perpendicularly to those on the other resistor sheets, wherein the resistor sheets have a two resistor layer construction comprising (a) a main resistor layer consisting of a thin metal film deposited thereon which in turn is bonded to (b) an electrically insulating layer and (c) a protective resistor layer formed on the surface of the main resistor layer.
摘要翻译： 一种电阻片输入片，包括两个矩形电阻片，每个矩形电阻片的两个相对边缘设置有电极，所述矩形电阻片重叠，使得其中一个电阻片上的电极垂直于另一个电阻片上的电极，其中电阻片 具有两个电阻层结构，包括：（a）由沉积在其上的薄金属膜构成的主电阻层，其又结合到（b）电绝缘层，和（c）形成在主体表面上的保护电阻层 电阻层。
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10.发明授权
公开(公告)号：US5541369A
公开(公告)日：1996-07-30
申请号：US464567
申请日：1995-06-05
申请人： Hiroshi Tahara , Seiju Kobayashi , Hitoshi Ohta
发明人： Hiroshi Tahara , Seiju Kobayashi , Hitoshi Ohta
CPC分类号： H05K1/0265 , H05K1/0237 , H05K1/0393 , H05K1/118 , H05K2201/0784 , H05K2201/09727 , H05K2201/09736
摘要： A printed circuit board having juxtaposed thereon in parallel plural conductive paths, at least a part of which has a different length, extending from one end side to the other end side, wherein the electric resistances of the conductive paths are made substantially same by changing the width of at least a part of the conductive paths.
摘要翻译： 一种印刷电路板，其平行地并置有多个导电路径，其至少一部分具有不同的长度，从一端侧延伸到另一端侧，其中通过改变导电路径使导电路径的电阻基本相同 至少一部分导电路径的宽度。
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