公开(公告)号：US20100083408A1

公开(公告)日：2010-04-01

申请号：US12626962

申请日：2009-11-30

申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N5/10 ,  C07H21/00

CPC分类号： C12N15/8216

摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

公开(公告)号：US07790874B2

公开(公告)日：2010-09-07

申请号：US12626962

申请日：2009-11-30

申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

IPC分类号： C12N15/63 ,  C12N15/67 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8216

摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

公开(公告)号：US20100077514A1

公开(公告)日：2010-03-25

申请号：US12626960

申请日：2009-11-30

申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

IPC分类号： A01H5/00 ,  C07H21/00 ,  C12N5/10

CPC分类号： C12N15/8216

摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

公开(公告)号：US07655786B2

公开(公告)日：2010-02-02

申请号：US11685347

申请日：2007-03-13

申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

IPC分类号： C07H21/04 ,  C12N15/00

CPC分类号： C12N15/8216

摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

摘要翻译： 本发明提供了筛选用于获得基因表达调节元件和基因绝缘子元件的方法。 本发明包括通过以下步骤鉴定基因表达调节元件和基因绝缘子元件的方法：a）定位两侧具有不同基因表达的每侧基因的植物基因组的基因间区域b）取该基因 区域或该基因间区域的一部分，并将其加入到包含分离的基因的盒中c）将盒导入植物细胞中d）分析分离的基因的表达。 本发明还包括用作基因表达调节元件的鉴定序列。

公开(公告)号：US07825234B2

公开(公告)日：2010-11-02

申请号：US12626961

申请日：2009-11-30

申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

IPC分类号： C12N15/63 ,  C12N15/67 ,  C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8216

摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

公开(公告)号：US20100083409A1

公开(公告)日：2010-04-01

申请号：US12626961

申请日：2009-11-30

申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

IPC分类号： A01H5/00 ,  C07H21/00 ,  C12N5/10

CPC分类号： C12N15/8216

摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

摘要翻译： 本发明提供了筛选用于获得基因表达调节元件和基因绝缘子元件的方法。 本发明包括通过以下步骤鉴定基因表达调节元件和基因绝缘子元件的方法：a）定位两侧具有不同基因表达的每侧基因的植物基因组的基因间区域b）取该基因 区域或该基因间区域的一部分，并将其加入到包含分离的基因的盒中c）将盒导入植物细胞中d）分析分离的基因的表达。 本发明还包括用作基因表达调节元件的鉴定序列。

公开(公告)号：US20080216201A1

公开(公告)日：2008-09-04

申请号：US11685347

申请日：2007-03-13

申请人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

发明人： Hochul Yi ,  Theodore M. Klein ,  Joanne E. Barton

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/11 ,  C12Q1/68 ,  C12N5/10

CPC分类号： C12N15/8216

摘要： The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.

摘要翻译： 本发明提供了筛选用于获得基因表达调节元件和基因绝缘子元件的方法。 本发明包括通过以下步骤鉴定基因表达调节元件和基因绝缘子元件的方法：a）定位两侧具有不同基因表达的每侧基因的植物基因组的基因间区域b）取该基因 区域或该基因间区域的一部分，并将其加入到包含分离的基因的盒中c）将盒导入植物细胞中d）分析分离的基因的表达。 本发明还包括用作基因表达调节元件的鉴定序列。