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1.发明授权
公开(公告)号：US4273866A
公开(公告)日：1981-06-16
申请号：US9007
申请日：1979-02-05
IPC分类号： C07F9/40 , C12Q1/46 , G01N33/532 , G01N33/542 , G01N33/54 , C12N9/99
CPC分类号： G01N33/542 , C07F9/4075 , G01N33/532 , Y10S435/81 , Y10S435/962 , Y10S436/825
摘要： The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction.The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.
摘要翻译： 本发明包括用于测定测试样品中配体的方法，包括与测试样品混合配体类似物不可逆酶抑制剂缀合物和可与该配体结合的结合蛋白和配体类似物不可逆酶抑制剂缀合物，其中配体类似物 由结合蛋白结合的可逆酶抑制剂缀合物与测试样品中配体的量相关，所述结合蛋白在结合到缀合物的配体类似物部分时使不可逆酶抑制剂失活; 混合由结合蛋白未结合的配体模拟不可逆酶抑制剂缀合物不可逆地抑制的酶; 并将底物与酶混合并监测酶底物反应。 本发明还包括在实施该方法中用作试剂的配体类似物不可逆酶抑制剂缀合物。 本发明的方法和试剂特别可用于确定生物流体中的药物，激素等。
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公开(公告)号：US4550163A
公开(公告)日：1985-10-29
申请号：US228414
申请日：1981-01-26
IPC分类号： C07F9/40 , G01N33/532 , G01N33/542 , C07F9/60
CPC分类号： G01N33/542 , C07F9/4075 , G01N33/532
摘要： The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction.The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.
摘要翻译： 本发明包括用于测定测试样品中配体的方法，包括与测试样品混合配体类似物不可逆酶抑制剂缀合物和可与该配体结合的结合蛋白和配体类似物不可逆酶抑制剂缀合物，其中配体类似物 由结合蛋白结合的可逆酶抑制剂缀合物与测试样品中配体的量相关，所述结合蛋白在结合到缀合物的配体类似物部分时使不可逆酶抑制剂失活; 混合由结合蛋白未结合的配体模拟不可逆酶抑制剂缀合物不可逆地抑制的酶; 并将底物与酶混合并监测酶底物反应。 本发明还包括在实施该方法中用作试剂的配体类似物不可逆酶抑制剂缀合物。 本发明的方法和试剂特别可用于确定生物流体中的药物，激素等。
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公开(公告)号：US4161488A
公开(公告)日：1979-07-17
申请号：US934297
申请日：1978-08-17
IPC分类号： C12Q1/37 , C12Q1/56 , C07C153/11 , C07C101/00
摘要： The present invention encompasses the acid addition salts of a compound of the formula ##STR1## wherein R.sub.1 represents phenylsulfonyl, benzoyl, carbobenzoxy, and the halo, loweralkyl having 1-4 carbon atoms, loweralkoxy having 1-3 carbon atoms, phenyl, or hydroxy substituted derivatives thereof; or alkanoyl having 2-12 carbon atoms; and R.sub.2 represents alkyl having 1-10 carbon atoms, or alkoxyalkyl having 2-6 carbon atoms, cycloalkyl having 5-7 carbon atoms, or benzyl and the halo, loweralkyl having 1-4 carbon atoms, loweralkoxy having 1-3 carbon atoms, hydroxy, carboxy, or phenyl substituted derivatives thereof.Compounds of the present invention are useful as analytical reagents. Enzymatic hydrolysis provides --S--R.sub.2 which can be further reacted with 5,5'-dithiobis (2-nitrobenzoic acid) to provide a colored product by which the enzyme concentration can be determined spectrophotometrically.
摘要翻译： 本发明包括下式化合物的酸加成盐：其中R 1表示苯基磺酰基，苯甲酰基，苄氧羰基，卤素，具有1-4个碳原子的低级烷基，具有1-3个碳原子的低级烷氧基，苯基或羟基 取代衍生物; 或具有2-12个碳原子的烷酰基; R2表示具有1-10个碳原子的烷基或具有2-6个碳原子的烷氧基烷基，具有5-7个碳原子的环烷基，或苄基和卤素，具有1-4个碳原子的低级烷基，具有1-3个碳原子的低级烷氧基， 羟基，羧基或其苯基取代的衍生物。
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公开(公告)号：US4997443A
公开(公告)日：1991-03-05
申请号：US445563
申请日：1989-12-04
CPC分类号： A61L27/22 , A61K35/39 , A61K9/1652 , A61K9/1658 , A61K9/1694 , A61L27/60
摘要： A transplantable artificial tissue matrix structure containing viable cells which is suitable for insertion into the body is made by polymerizing precursors in an aqueous solution to form a shape retaining solid matrix comprising viable cells, matrix polymer and reversible gel polymer. The solution contains a matrix polymer precursor, a reversible gel polymer precursor, and viable cells. The reversible gel polymer is dissolved and removed to yield an insoluble, porous matrix containing viable cells. The conditions and reagents are selected to maintain the viability of the cells. The invention is particularly suitable for artificial transplant matrix tissue containing pancreatic islet cells.
摘要翻译： 通过在水溶液中聚合前体以形成保持包含活细胞，基质聚合物和可逆凝胶聚合物的固体基质的形状来制备包含适于插入体内的活细胞的可移植的人造组织基质结构。 该溶液含有基质聚合物前体，可逆的凝胶聚合物前体和活细胞。 将可逆的凝胶聚合物溶解并除去，得到含有活细胞的不溶性多孔基质。 选择条件和试剂以维持细胞的活力。 本发明特别适用于含有胰岛细胞的人造移植基质组织。
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公开(公告)号：US4902295A
公开(公告)日：1990-02-20
申请号：US118280
申请日：1987-11-06
CPC分类号： A61K9/1694 , A61K35/39 , A61K9/1652 , A61K9/1658 , A61L27/22 , A61L27/60
摘要： A transplantable artificial tissue matrix structure containing viable cells which is suitable for insertion into the body is made by polymerizing precursors in an aqueous solution to form a shape retaining solid matrix comprising viable cells, matrix polymer and reversible gel polymer. The solution contains a matrix polymer precursor, a reversible gel polymer precursor, and viable cells. The reversible gel polymer is dissolved and removed to yield an insoluble, porous matrix containing viable cells. The conditions and reagents are selected to maintain the viability of the cells. The invention is particularly suitable for artificial transplant matrix tissue containing pancreatic islet cells.
摘要翻译： 通过在水溶液中聚合前体以形成保持包含活细胞，基质聚合物和可逆凝胶聚合物的固体基质的形状来制备包含适于插入体内的活细胞的可移植的人造组织基质结构。 该溶液含有基质聚合物前体，可逆的凝胶聚合物前体和活细胞。 将可逆的凝胶聚合物溶解并除去，得到含有活细胞的不溶性多孔基质。 选择条件和试剂以维持细胞的活力。 本发明特别适用于含有胰岛细胞的人造移植基质组织。
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公开(公告)号：USD512512S1
公开(公告)日：2005-12-06
申请号：US29182200
申请日：2003-05-21
申请人： Douglas E. Bell , Gary T. Neel , Houston F. Voss
设计人： Douglas E. Bell , Gary T. Neel , Houston F. Voss
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公开(公告)号：US4166825A
公开(公告)日：1979-09-04
申请号：US934298
申请日：1978-08-17
IPC分类号： C12Q1/37 , C07C153/09
CPC分类号： C12Q1/37
摘要： The present invention encompasses acid addition salts of a compound of the formula ##STR1## wherein: R.sub.1 represents phenylsulfonyl, benzoyl, carbobenzoxy, and the halo, loweralkyl having 1-4 carbon atoms, loweralkoxy having 1-3 carbon atoms, phenyl, or hydroxy substituted derivatives thereof; or alkanoyl having 2-12 carbon atoms; R.sub.2 represents alkyl having 1-10 carbon atoms, alkoxyalkyl having 2-6 carbon atoms, cycloalkyl having 5-7 carbon atoms, benzyl and the halo, loweralkyl having 1-4 carbon atoms, loweralkoxy having 1-3 carbon atoms, carboxy, hydroxy, or substituted derivatives thereof; R.sub.3 represents hydrogen or guanyl, and m is 3 or 4.Compounds of the present invention are useful as analytical reagents. Enzymatic hydrolysis provides --S--R.sub.2 which can be further reacted with 5,5'-dithiobis (2-nitrobenzoic acid) to provide a colored product by which the enzyme concentration can be determined spectrophotometrically.
摘要翻译： 本发明包括下式化合物的酸加成盐：其中：R 1表示苯基磺酰基，苯甲酰基，苄氧羰基，卤素，具有1-4个碳原子的低级烷基，具有1-3个碳原子的低级烷氧基，苯基或羟基 取代衍生物; 或具有2-12个碳原子的烷酰基; R2表示具有1-10个碳原子的烷基，具有2-6个碳原子的烷氧基烷基，具有5-7个碳原子的环烷基，苄基和卤素，具有1-4个碳原子的低级烷基，具有1-3个碳原子的低级烷氧基，羧基，羟基 或其取代的衍生物; R3表示氢或脒基，m表示3或4。
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公开(公告)号：US4341865A
公开(公告)日：1982-07-27
申请号：US170361
申请日：1980-07-21
申请人： Houston F. Voss
发明人： Houston F. Voss
IPC分类号： C07F9/40 , G01N33/542 , G01N33/78 , G01N33/54
CPC分类号： G01N33/78 , C07F9/4075 , G01N33/542 , Y10S435/81
摘要： This invention encompasses a method for measuring unsaturated thyroxine binding globulin in serum comprising intermixing with the serum:(i) thyroxine irreversible enzyme inhibitor conjugate which binds to the unsaturated thyroxine binding globulin thereby inactivating the irreversible enzyme inhibitor portion of the conjugate;(ii) an enzyme which is irreversibly inhibited by the thyroxine irreversible enzyme inhibitor conjugate unbound by the thyroxine binding globulin; and(iii) a substrate for the enzyme; and monitoring the enzyme substrate reaction.
摘要翻译： 本发明包括用于测量血清中不饱和甲状腺素结合球蛋白的方法，包括与血清混合：（i）结合不饱和甲状腺素结合球蛋白的甲状腺素不可逆酶抑制剂缀合物，从而使缀合物的不可逆酶抑制剂部分失活; （ii）由甲状腺素结合球蛋白未结合的甲状腺素不可逆酶抑制剂缀合物不可逆地抑制的酶; 和（iii）酶的底物; 并监测酶底物反应。
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公开(公告)号：US07819161B2
公开(公告)日：2010-10-26
申请号：US11590854
申请日：2006-11-01
申请人： Gary T. Neel , Douglas E. Bell , T. Philip Wong , Houston F. Voss , Allan Javier Caban , David K. Boehm
发明人： Gary T. Neel , Douglas E. Bell , T. Philip Wong , Houston F. Voss , Allan Javier Caban , David K. Boehm
IPC分类号： G01N27/327
CPC分类号： C12Q1/006 , G01N27/3272 , Y10T29/49002 , Y10T156/12 , Y10T436/104998 , Y10T436/144444
摘要： A system for measuring a glucose level in a blood sample includes a test strip and a meter. The test strip includes a sample chamber, a working electrode, a counter electrode, fill-detect electrodes, and an auto-on conductor. A reagent layer is disposed in the sample chamber. The auto-on conductor causes the meter to wake up and perform a test strip sequence when the test strip is inserted in the meter. The meter uses the working and counter electrodes to initially detect the blood sample in the sample chamber and uses the fill-detect electrodes to check that the blood sample has mixed with the reagent layer. The meter applies an assay voltage between the working and counter electrodes and measures the resulting current. The meter calculates the glucose level based on the measured current and calibration data saved in memory from a removable data storage device associated with the test strip.
摘要翻译： 用于测量血液样品中的葡萄糖水平的系统包括测试条和米。 测试条包括样品室，工作电极，对电极，填充检测电极和自动导体。 试剂层设置在样品室中。 当测试条插入仪表时，自动导体导致仪表醒来并执行测试条序列。 仪表使用工作电极和对电极初始检测样品室中的血液样品，并使用填充检测电极检查血液样品是否与试剂层混合。 仪表在工作电极和对电极之间施加测定电压并测量所得电流。 仪表根据与测试条相关联的可移动数据存储设备中保存在存储器中的测量电流和校准数据计算葡萄糖水平。
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公开(公告)号：US06946299B2
公开(公告)日：2005-09-20
申请号：US10420995
申请日：2003-04-21
申请人： Gary T. Neel , Douglas E. Bell , T. Philip Wong , Houston F. Voss , Allan Javier Caban , David K. Boehm
发明人： Gary T. Neel , Douglas E. Bell , T. Philip Wong , Houston F. Voss , Allan Javier Caban , David K. Boehm
IPC分类号： A61K20060101 , C12Q1/00 , G01N27/00 , G01N27/26 , G01N33/00 , G01N33/487 , G01N33/543 , G01N33/66
CPC分类号： C12Q1/006 , A61B5/14532 , A61B2560/0475 , G01N27/3272 , G01N33/54373 , G01N33/66 , Y10T436/104998 , Y10T436/144444
摘要： A system for measuring a glucose level in a blood sample includes a test strip and a meter. The test strip includes a sample chamber, a working electrode, a counter electrode, fill-detect electrodes, and an auto-on conductor. A reagent layer is disposed in the sample chamber. The auto-on conductor causes the meter to wake up and perform a test strip sequence when the test strip is inserted in the meter. The meter uses the working and counter electrodes to initially detect the blood sample in the sample chamber and uses the fill-detect electrodes to check that the blood sample has mixed with the reagent layer. The meter applies an assay voltage between the working and counter electrodes and measures the resulting current. The meter calculates the glucose level based on the measured current and calibration data saved in memory from a removable data storage device associated with the test strip.
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