利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

33 条记录 (耗时 0.024 秒)

    Method of amplifying nucleic acid from a cell using a nonplanar solid substrate
    1.
    发明授权
    Method of amplifying nucleic acid from a cell using a nonplanar solid substrate 有权
    使用非平面固体底物从细胞中扩增核酸的方法

    公开(公告)号：US07919278B2

    公开(公告)日：2011-04-05

    申请号：US11841107

    申请日：2007-08-20

    申请人： Sung-young Jeong ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Jung-im Han ,  Hun-joo Lee

    发明人： Sung-young Jeong ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Jung-im Han ,  Hun-joo Lee

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/1006 ,  B01L7/52 ,  B01L2300/0636 ,  C12N2533/30 ,  C12Q1/6806

    摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.

    摘要翻译： 本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。

    Microfluidic device and method for concentrating sample containing cells or viruses and lysing cells or viruses, and method of producing the microfluidic device
    2.
    发明授权
    Microfluidic device and method for concentrating sample containing cells or viruses and lysing cells or viruses, and method of producing the microfluidic device 有权
    用于浓缩含有细胞或病毒的样品和裂解细胞或病毒的微流体装置和方法以及产生微流体装置的方法

    公开(公告)号：US07955842B2

    公开(公告)日：2011-06-07

    申请号：US11670002

    申请日：2007-02-01

    申请人： Hun-joo Lee ,  Joon-ho Kim ,  Sung-young Jeong ,  Kyu-youn Hwang

    发明人： Hun-joo Lee ,  Joon-ho Kim ,  Sung-young Jeong ,  Kyu-youn Hwang

    IPC分类号： C12M1/42 ,  C12M1/33

    CPC分类号： C12M47/02 ,  C12M47/06

    摘要： A microfluidic device for concentrating a sample including cells or viruses and lysing the cells or viruses, the device including an anode chamber including an anode electrode, a cathode chamber including a cathode electrode and an ion exchange membrane separating the anode chamber and the cathode chamber. The cathode chamber includes a solid support therein. A method of producing the microfluidic device and a method of concentrating a sample including cells or viruses and lysing the cells or viruses therein using the microfluidic device.

    摘要翻译： 一种用于浓缩包含细胞或病毒的样品并裂解细胞或病毒的微流体装置，所述装置包括包括阳极电极的阳极室，包括阴极的阴极室和分离阳极室和阴极室的离子交换膜。 阴极室包括固体支撑体。 微流体装置的制造方法以及浓缩包含细胞或病毒的样品并使用微流体装置使细胞或病毒裂解的方法。

    MICROFLUIDIC DEVICE AND METHOD OF FABRICATING THE SAME
    7.
    发明申请
    MICROFLUIDIC DEVICE AND METHOD OF FABRICATING THE SAME 审中-公开
    微流体装置及其制造方法

    公开(公告)号：US20070160502A1

    公开(公告)日：2007-07-12

    申请号：US11616148

    申请日：2006-12-26

    申请人： Kyu-youn Hwang ,  Sung-young Jeong ,  Joon-ho KIM ,  Hun-joo LEE ,  Hee-kyun LIM

    发明人： Kyu-youn Hwang ,  Sung-young Jeong ,  Joon-ho KIM ,  Hun-joo LEE ,  Hee-kyun LIM

    IPC分类号： B32B37/12 ,  B32B38/04 ,  B01L3/00

    CPC分类号： B01L3/502707 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0887 ,  Y10T156/1052

    摘要： Disclosed herein are a method of fabricating a microfluidic device, and a microfluidic device fabricated by the method. The method includes coating an adhesive material on a first substrate having a fluid port to form an adhesive layer thereon, arranging a second substrate having a microstructure formed therein with the surface of the first substrate on which the adhesive layer is formed, such that the fluid port and the microstructure correspond to each other, and heating the substrates at about 50 to about 180 degrees Celsius to bind the first substrate to the second substrate.

    摘要翻译： 本文公开了一种制造微流体装置的方法，以及通过该方法制造的微流体装置。 该方法包括在具有流体端口的第一基板上涂覆粘合剂材料以在其上形成粘合剂层，将其中形成有微结构的第二基板与其上形成有粘合剂层的第一基板的表面布置，使得流体 端口和微结构彼此​​对应，并且在约50至约180摄氏度下加热基板以将第一基板结合到第二基板。

    Microfluidic chip and method of fabricating the same
    9.
    发明授权
    Microfluidic chip and method of fabricating the same 有权
    微流控芯片及其制造方法

    公开(公告)号：US07858042B2

    公开(公告)日：2010-12-28

    申请号：US11934811

    申请日：2007-11-05

    申请人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chin-sung Park ,  Sung-young Jeong

    发明人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chin-sung Park ,  Sung-young Jeong

    IPC分类号： B01N3/00

    CPC分类号： B01L3/502707 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2300/16 ,  Y10T428/14

    摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

    摘要翻译： 提供了一种微流控芯片及其制造方法。 微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。

    Microfluidic chip and method of fabricating the same
    10.
    发明授权
    Microfluidic chip and method of fabricating the same 有权
    微流控芯片及其制造方法

    公开(公告)号：US08153085B2

    公开(公告)日：2012-04-10

    申请号：US12949981

    申请日：2010-11-19

    申请人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chin-sung Park ,  Sung-young Jeong

    发明人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chin-sung Park ,  Sung-young Jeong

    IPC分类号： B01L3/00

    CPC分类号： B01L3/502707 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2300/16 ,  Y10T428/14

    摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

    摘要翻译： 提供了一种微流控芯片及其制造方法。 微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。