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公开(公告)号：US12083514B2
公开(公告)日：2024-09-10
申请号：US17727622
申请日：2022-04-22
Applicant: ILLUMINA, Inc.
Inventor： Maxwell Zimmerley , LiangLiang Qiang , M. Shane Bowen , Steven H. Modiano , Dajun Yuan , Randall Smith , Arthur J. Pitera , Hai Quang Tran , Gerald Kreindl
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/502707 , B01L3/502746
Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.
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公开(公告)号：US11667969B2
公开(公告)日：2023-06-06
申请号：US15969562
申请日：2018-05-02
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED , ILLUMINA SINGAPORE PTE. LTD.
Inventor： Sean M. Ramirez , Brian D. Mather , Edwin Li , Sojeong Moon , Innsu Daniel Kim , Alexandre Richez , Ludovic Vincent , Xavier von Hatten , Hai Quang Tran , Maxwell Zimmerley , Julia Morrison , Gianluca Andrea Artioli , Krystal Sly , Hayden Black , Lewis J. Kraft , Hong Xie , Wei Wei , Ryan Sanford
IPC: B01L3/00 , C12Q1/6874 , C12Q1/6806
CPC classification number: C12Q1/6874 , B01L3/502707 , C12Q1/6806 , B01L2300/0819 , B01L2300/0877 , B01L2300/0887 , B01L2300/16 , B01L2300/165
Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.
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公开(公告)号：US10861829B2
公开(公告)日：2020-12-08
申请号：US16609554
申请日：2017-12-26
Applicant: ILLUMINA, INC.
Inventor： Tracy Helen Fung , Hai Quang Tran
IPC: G01N21/64 , H01L25/065 , H01L23/31 , H01L23/00
Abstract: A system includes an image sensor structure and a flow cell. The image sensor structure includes an image layer disposed over a base substrate. A device stack is disposed over the image layer. A bond pad is disposed in the device stack. A passivation stack is disposed over the device stack and the bond pad. An array of nanowells is disposed in a top layer of the passivation stack. A through-silicon via (TSV) is in electrical contact with the bond pad. The TSV extends through the base substrate. A redistribution layer (RDL) is disposed on a bottom surface of the base substrate. The RDL is in electrical contact with the TSV. The flow cell is disposed upon the top layer of the passivation stack to form a flow channel therebetween. The flow channel is disposed over the array of nanowells and the bond pad.
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4.发明授权
公开(公告)号：US11590494B2
公开(公告)日：2023-02-28
申请号：US16433969
申请日：2019-06-06
Applicant: Illumina, inc.
Inventor： Alex Aravanis , Boyan Boyanov , M. Shane Bowen , Dale Buermann , Alexander Hsiao , Behnam Javanmardi , Tarun Khurana , Poorya Sabounchi , Hai Quang Tran
Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.
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公开(公告)号：US20220250066A1
公开(公告)日：2022-08-11
申请号：US17727622
申请日：2022-04-22
Applicant: ILLUMINA, Inc.
Inventor： Maxwell Zimmerley , LiangLiang Qiang , M. Shane Bowen , Steven H. Modiano , Dajun Yuan , Randall Smith , Arthur J. Pitera , Hai Quang Tran , Gerald Kreindl
IPC: B01L3/00
Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.
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6.发明申请
公开(公告)号：US20190283024A1
公开(公告)日：2019-09-19
申请号：US16433969
申请日：2019-06-06
Applicant: Illumina, Inc.
Inventor： Alex Aravanis , Boyan Boyanov , M. Shane Bowen , Dale Buermann , Alexander Hsiao , Behnam Javanmardi , Tarun Khurana , Poorya Sabounchi , Hai Quang Tran
Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.
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公开(公告)号：US20180327832A1
公开(公告)日：2018-11-15
申请号：US15969562
申请日：2018-05-02
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED , ILLUMINA SINGAPORE PTE. LTD.
Inventor： Sean M. Ramirez , Brian D. Mather , Edwin Li , Sojeong Moon , Innsu Daniel Kim , Alexandre Richez , Ludovic Vincent , Xavier von Hatten , Hai Quang Tran , Maxwell Zimmerley , Julia Morrison , Gianluca Andrea Artioli , Krystal Sly , Hayden Black , Lewis J. Kraft , Hong Xie , Wei Wei , Ryan Sanford
IPC: C12Q1/6874 , C12Q1/6806
CPC classification number: C12Q1/6874 , C12Q1/6806
Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.
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8.发明公开
公开(公告)号：US20240001361A1
公开(公告)日：2024-01-04
申请号：US18369587
申请日：2023-09-18
Applicant: ILLUMINA, INC.
Inventor： Sebastian Bohm , Alex Aravanis , Alexander Hsiao , Behnam Javanmardi , Tarun Kumar Khurana , Hai Quang Tran , Majid Aghababazadeh , M. Shane Bowen , Boyan Boyanov , Dale Buermann
IPC: B01L3/00 , B01L7/00 , F16K99/00 , G01N35/08 , C12Q1/6869
CPC classification number: B01L3/502738 , B01L3/50273 , B01L7/52 , F16K99/0013 , F16K99/0028 , F16K99/0015 , G01N35/08 , B01L3/502715 , C12Q1/6869 , B01L2400/0644 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2400/0487 , F16K2099/0084 , B01L2400/0655
Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.
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公开(公告)号：US11784161B2
公开(公告)日：2023-10-10
申请号：US16949529
申请日：2020-11-02
Applicant: Illumina, Inc.
Inventor： Tracy Helen Fung , Hai Quang Tran
IPC: H01L25/065 , H01L23/31 , H01L23/00
CPC classification number: H01L25/0657 , H01L23/3135 , H01L24/29 , H01L24/45 , H01L24/91 , H01L2224/02372 , H01L2225/06541
Abstract: A system includes an image sensor structure and a flow cell. The image sensor structure includes an image layer disposed over a base substrate. A device stack is disposed over the image layer. A bond pad is disposed in the device stack. A passivation stack is disposed over the device stack and the bond pad. An array of nanowells is disposed in a top layer of the passivation stack. A through-silicon via (TSV) is in electrical contact with the bond pad. The TSV extends through the base substrate. A redistribution layer (RDL) is disposed on a bottom surface of the base substrate. The RDL is in electrical contact with the TSV. The flow cell is disposed upon the top layer of the passivation stack to form a flow channel therebetween. The flow channel is disposed over the array of nanowells and the bond pad.
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10.发明授权
公开(公告)号：US11786898B2
公开(公告)日：2023-10-17
申请号：US16818953
申请日：2020-03-13
Applicant: Illumina, Inc.
Inventor： Sebastian Bohm , Alex Aravanis , Alexander Hsiao , Behnam Javanmardi , Tarun Khurana , Hai Quang Tran , Majid Aghababazadeh , M. Shane Bowen , Boyan Boyanov , Dale Buermann
IPC: B01L3/00 , B01L7/00 , F16K99/00 , G01N35/08 , C12Q1/6869
CPC classification number: B01L3/502738 , B01L3/50273 , B01L3/502715 , B01L7/52 , C12Q1/6869 , F16K99/0013 , F16K99/0015 , F16K99/0028 , G01N35/08 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2400/0487 , B01L2400/0644 , B01L2400/0655 , F16K2099/0084
Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.
