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公开(公告)号：US08105814B2
公开(公告)日：2012-01-31
申请号：US12868359
申请日：2010-08-25
申请人： Ikuo Matsui , Yuji Urushibata
发明人： Ikuo Matsui , Yuji Urushibata
摘要： A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.
摘要翻译： DNA聚合酶反应体系即使在高温和高盐浓度下也能提供高DNA聚合酶活性。 由DNA聚合酶，钳夹和没有内含肽序列的钳夹装置构建的DNA聚合酶反应系统，DNA聚合酶来自热球菌Pyococcus horikoshii，一种超嗜热古细菌。
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公开(公告)号：US20070264660A1
公开(公告)日：2007-11-15
申请号：US11748961
申请日：2007-05-15
申请人： Ikuo Matsui , Yuji Urushibata
发明人： Ikuo Matsui , Yuji Urushibata
摘要： A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.
摘要翻译： DNA聚合酶反应体系即使在高温和高盐浓度下也能提供高DNA聚合酶活性。 由DNA聚合酶，钳夹和没有内含肽序列的钳夹装置构建的DNA聚合酶反应系统，DNA聚合酶来自热球菌Pyococcus horikoshii，一种超嗜热古细菌。
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公开(公告)号：US07824886B2
公开(公告)日：2010-11-02
申请号：US11748961
申请日：2007-05-15
申请人： Ikuo Matsui , Yuji Urushibata
发明人： Ikuo Matsui , Yuji Urushibata
摘要： A DNA polymerase reaction system which provides high DNA polymerase activity even at a high temperature and at a high salt concentration. A DNA polymerase reaction system that is constructed from a DNA polymerase, a clamp, and a clamp loader without intein sequence, the DNA polymerase being from Pyrococcus horikoshii, a hyperthermophilic archaeon.
摘要翻译： DNA聚合酶反应体系即使在高温和高盐浓度下也能提供高DNA聚合酶活性。 由DNA聚合酶，钳夹和没有内含肽序列的钳夹装置构建的DNA聚合酶反应系统，DNA聚合酶来自热球菌Pyococcus horikoshii，一种超嗜热古细菌。
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公开(公告)号：US06960454B2
公开(公告)日：2005-11-01
申请号：US09369735
申请日：1999-08-06
IPC分类号： C12N15/09 , C12N1/21 , C12N9/24 , C12N9/42 , C12N15/00 , C12R1/01 , C12R1/19 , C12P19/04 , C07K1/00 , C12N9/26 , C12P19/02
CPC分类号： C12Y302/01021 , C12N9/2402
摘要： The invention relates to a thermophilic enzyme having β-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.
摘要翻译： 本发明涉及具有β-糖苷酶活性的嗜热酶，其包含SEQ ID NO：2的氨基酸序列，其中可以缺失，替换或添加一个或多个氨基酸残基。
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5.发明授权Thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity 失效具有磷脂酰乙醇胺N-甲基转移酶活性的热稳定酶公开(公告)号：US06391604B1
公开(公告)日：2002-05-21
申请号：US09519729
申请日：2000-03-03
IPC分类号： C12N910
CPC分类号： C12N9/1007 , C12Y201/01017 , Y10S435/822
摘要： A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90° C. or higher, and the transformed host cell is cultured.
摘要翻译： 提供了热稳定性磷脂酰乙醇胺N-甲基转移酶及其制备方法。 宿主细胞用含有编码来源于嗜热古细菌的热稳定酶的DNA的表达载体转化，如Pyrococcus，具有磷脂酰乙醇胺N-甲基转移酶活性的酶，最适温度为90℃或更高，转化的宿主细胞 被培养
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公开(公告)号：US6133012A
公开(公告)日：2000-10-17
申请号：US16080
申请日：1998-01-30
IPC分类号： C12N15/09 , C07H21/04 , C12N9/48 , C12N9/78 , C12N15/00 , C12Q1/37 , C12R1/01 , C12R1/19 , C12N9/52
CPC分类号： C12N9/48
摘要： An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.
摘要翻译： 公开了具有最佳温度范围为90-95℃的酰基肽水解酶和编码该酰基肽水解酶的基因。 利用上述酶，可以在高温下进行酰化蛋白质和肽的氨基末端分析。
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公开(公告)号：US5635378A
公开(公告)日：1997-06-03
申请号：US467831
申请日：1995-06-06
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
IPC分类号： C12N9/10 , C12N9/30 , C12N15/09 , C12N15/54 , C12P19/14 , C12P19/18 , C12R1/07 , C12R1/125 , C12R1/645 , C12R1/865 , C12P19/00
CPC分类号： C12N9/242 , C12N9/1074 , C12P19/18 , Y10S435/832
摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
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8.发明授权Variant-type carbohydrate hydrolase, variant gene of the enzyme and method for producing oligosaccharide using the enzyme 失效变体型碳水化合物水解酶，酶的变体基因和使用该酶生产寡糖的方法
公开(公告)号：US5631149A
公开(公告)日：1997-05-20
申请号：US470702
申请日：1995-06-06
申请人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
发明人： Ikuo Matsui , Kazuhiko Ishikawa , Sachio Miyairi , Koichi Honda
IPC分类号： C12N9/10 , C12N9/30 , C12N15/09 , C12N15/54 , C12P19/14 , C12P19/18 , C12R1/07 , C12R1/125 , C12R1/645 , C12R1/865 , C12P19/04
CPC分类号： C12N9/242 , C12N9/1074 , C12P19/18 , Y10S435/832
摘要： There is disclosed a variant-type carbohydrate hydrolase that has been increased transglycosylation activity by substituting another amino acid residue for the tyrosine residue that is present in the active center of the hydrolase, which hydrolase is an amylase or an enzyme analogous to amylase; a gene or a DNA sequence of the carbohydrate hydrolase with mutation introduced into the base sequence that encodes the tyrosine residue; and a vector or a transformant which comprises the DNA sequence. There is also disclosed a method for producing a variety of oligosaccharides and the like by using the variant-type carbohydrate hydrolase.
摘要翻译： 公开了通过用另一个氨基酸残基取代存在于水解酶的活性中心的酪氨酸残基而已经增加转糖基化活性的变体型碳水化合物水解酶，该水解酶是淀粉酶或类似于淀粉酶的酶; 具有导入编码酪氨酸残基的碱基序列的突变的碳水化合物水解酶的基因或DNA序列; 以及包含DNA序列的载体或转化体。 还公开了通过使用变体型碳水化合物水解酶来生产各种寡糖等的方法。
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公开(公告)号：US20080118922A1
公开(公告)日：2008-05-22
申请号：US11658096
申请日：2005-01-21
申请人： Eriko Matsui , Ikuo Matsui
发明人： Eriko Matsui , Ikuo Matsui
CPC分类号： C12N9/22
摘要： A mutation is introduced into the substrate-binding site of flap endonuclease to prepare a mutant with modified substrate specificity. Using the mutant as a reagent for the analysis of genetic polymorphism, the analysis of genetic polymorphism can be performed more accurately, easily and sensitively as compared with conventional methods.
摘要翻译： 将突变引入皮瓣内切核酸酶的底物结合位点以制备具有修饰的底物特异性的突变体。 使用突变体作为遗传多态性分析的试剂，与常规方法相比，遗传多态性的分析可以更准确，更容易和灵敏地进行。
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公开(公告)号：US06939699B2
公开(公告)日：2005-09-06
申请号：US10373090
申请日：2003-02-26
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/90 , C12N15/61 , C12N9/10 , C12N9/14
CPC分类号： C12N9/90
摘要： The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such as Escherichia coli in the usual manner to create a transformant, and culturing the transformant.
摘要翻译： 本发明提供具有DNA解旋酶活性的热稳定酶，优选结构特异性内切核酸酶活性。 该酶可以通过将编码该酶的基因从超嗜热菌，优选硫代谢嗜热古细菌的染色体DNA克隆到表达载体中，将该基因导入表达载体中，将载体以常规方式引入宿主如大肠杆菌 创造转化体，培养转化体。
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