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公开(公告)号：US12174532B2
公开(公告)日：2024-12-24
申请号：US17255393
申请日：2020-05-28
Applicant: ILLUMINA, INC.
Inventor： Dajun Yuan , M. Shane Bowen , Zhong Mei
Abstract: Provided in one example is a method of manufacturing a flowcell that includes: forming a core layer, the core layer disposed between a substrate and a nanowell layer, the nanowell layer having nanowells to receive a sample, the core layer having a higher refractive index than the substrate and the nanowell layer; and forming a grating to couple light to the core layer.
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公开(公告)号：US12110547B2
公开(公告)日：2024-10-08
申请号：US17033237
申请日：2020-09-25
Applicant: Illumina, Inc.
Inventor： Andrew A. Brown , Wayne N. George , Alexandre Richez , M. Shane Bowen
CPC classification number: C12Q1/6874 , G03F7/0002 , G03F7/161 , B01L3/5085 , B01L2200/12 , B01L2300/0819 , B01L2300/0829 , B01L2300/161 , G01N21/645
Abstract: Substrates comprising dual functional polymer layered surfaces and the preparation thereof by using UV nano-imprinting processes are disclosed. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.
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公开(公告)号：US12083514B2
公开(公告)日：2024-09-10
申请号：US17727622
申请日：2022-04-22
Applicant: ILLUMINA, Inc.
Inventor： Maxwell Zimmerley , LiangLiang Qiang , M. Shane Bowen , Steven H. Modiano , Dajun Yuan , Randall Smith , Arthur J. Pitera , Hai Quang Tran , Gerald Kreindl
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/502707 , B01L3/502746
Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.
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公开(公告)号：US20240209412A1
公开(公告)日：2024-06-27
申请号：US18590738
申请日：2024-02-28
Applicant: ILLUMINA, INC.
Inventor： Wayne N. George , Alexandre Richez , M. Shane Bowen , Andrew A. Brown , Dajun Yuan , Audrey Rose Zak , Sean M. Ramirez , Raymond Campos
IPC: C12Q1/68 , C08G77/14 , C09D133/26 , C09D183/06
CPC classification number: C12Q1/68 , C09D133/26 , C09D183/06 , C08G77/14
Abstract: An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.
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公开(公告)号：US11680292B2
公开(公告)日：2023-06-20
申请号：US17840569
申请日：2022-06-14
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： Jeffrey S. Fisher , Brian D. Mather , Maria Candelaria Rogert Bacigalupo , Justin Fullerton , Ludovic Vincent , Lewis J. Kraft , Sahngki Hong , Boyan Boyanov , M. Shane Bowen , Sang Park , Wayne N. George , Andrew A. Brown
IPC: C12Q1/6869 , C08F220/56 , C08G77/04 , G03F7/075 , G03F7/16 , G03F7/00 , G03F7/004
CPC classification number: C12Q1/6869 , C08F220/56 , C08G77/04 , G03F7/0757 , G03F7/165 , G03F7/0002 , G03F7/0046
Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
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公开(公告)号：US20230149933A1
公开(公告)日：2023-05-18
申请号：US18154510
申请日：2023-01-13
Applicant: Illumina, Inc.
Inventor： M. Shane Bowen , Michael Graige , Stanley S. Hong , John A. Moon , Merek Siu
CPC classification number: B01L3/5085 , B01J19/0046 , B01J2219/00317 , B01J2219/00509 , B01J2219/00621 , B01J2219/00662 , B01J2219/00668 , B01J2219/00722 , B01L2300/0636 , B01L2300/0819 , B01L2300/0848 , B01L2300/0851 , C12Q1/6837
Abstract: An array including a solid support having a plurality of contours along its exterior surface. A first subset of contours is positioned along the exterior surface of the solid support to form a first pattern of features and a second subset of contours is positioned along the exterior surface to form a second pattern of features. The contours of the first subset are juxtaposed with the second subset along the exterior surface, whereby the first and second patterns form an interleaved pattern. The features of the first pattern occur at a first elevation z1 and the features of the second pattern occur at a second elevation z2. The features of the first pattern are configured to attach analytes at a different elevation relative to analytes attached to the features of the second pattern.
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公开(公告)号：US20230002822A1
公开(公告)日：2023-01-05
申请号：US17840569
申请日：2022-06-14
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： Jeffrey S. Fisher , Brian D. Mather , Maria Candelaria Rogert Bacigalupo , Justin Fullerton , Ludovic Vincent , Lewis J. Kraft , Sahngki Hong , Boyan Boyanov , M. Shane Bowen , Sang Park , Wayne N. George , Andrew A. Brown
IPC: C12Q1/6869 , C08F220/56 , C08G77/04 , G03F7/075 , G03F7/16
Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.
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公开(公告)号：US20220410146A1
公开(公告)日：2022-12-29
申请号：US17898155
申请日：2022-08-29
Applicant: ILLUMINA, INC.
Inventor： Tarun Kumar Khurana , Arnaud Rival , Lewis J. Kraft , Steven Barnard , M. Shane Bowen , Xi-Jun Chen , Yir-Shyuan Wu , Jeffrey S. Fisher , Dajun Yuan
Abstract: An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.
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公开(公告)号：US11512339B2
公开(公告)日：2022-11-29
申请号：US15848640
申请日：2017-12-20
Applicant: ILLUMINA, INC. , ILLUMINA CAMBRIDGE LIMITED
Inventor： Wayne N. George , Alexandre Richez , M. Shane Bowen , Andrew A. Brown , Dajun Yuan , Audrey Rose Zak , Sean M. Ramirez , Raymond Campos
IPC: C09D183/06 , C12Q1/68 , C09D133/26 , C08G77/14
Abstract: An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.
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公开(公告)号：US11442017B2
公开(公告)日：2022-09-13
申请号：US16949572
申请日：2020-11-04
Applicant: Illumina, Inc.
Inventor： John Gerhardt Earney , Joseph Francis Pinto , M. Shane Bowen , Michael S. Graige , Arthur Pitera , Bala Murali K. Venkatesan , Dajun Yuan
Abstract: An inspection apparatus is provided that comprises an optical target including a solid host material and a fluorescing material embedded in the solid host material. The solid host material has a predetermined phonon energy HOSTPE. The fluorescing material exhibits a select ground energy level and a target excitation (TE) energy level separated from the ground energy level by a first energy gap corresponding to a fluorescence emission wavelength of interest. The fluorescing material has a next lower lying (NLL) energy level relative to the TE energy level. The NLL energy level is spaced a second energy gap FMEG2 below the TE energy level, wherein a ratio of the FMEG2/HOSTPE is three or more.
