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公开(公告)号：US20220180970A1
公开(公告)日：2022-06-09
申请号：US17675295
申请日：2022-02-18
Applicant: ILLUMINA CAMBRIDGE LIMITED
Inventor： Roberto Rigatti , Jonathan Mark Boutell
IPC: G16B30/10 , G16B30/00 , C12Q1/6869 , B01L7/00 , G16C20/20
Abstract: The present disclosure provides methods and systems for determining and/or characterizing one or more haplotypes and/or phasing of haplotypes in a nucleic acid sample. In particular, the disclosure provides methods for determining a haplotype and/or phasing of haplotypes in a nucleic acid sample by incorporating synthetic polymorphisms into fragments of a nucleic acid sample and utilizing the synthetic polymorphisms in determining one or more haplotypes and/or phasing of haplotypes.
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公开(公告)号：US20200002747A1
公开(公告)日：2020-01-02
申请号：US16561483
申请日：2019-09-05
Applicant: Illumina Cambridge Limited
Inventor： Roberto Rigatti , Niall Anthony Gormley
IPC: C12Q1/6806 , C12Q1/6874
Abstract: Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid in contact with a surface, and sequencing the library on the surface
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公开(公告)号：US10329613B2
公开(公告)日：2019-06-25
申请号：US14683580
申请日：2015-04-10
Applicant: Illumina Cambridge Limited
Inventor： Roberto Rigatti , Niall Anthony Gormley , Jonathan Mark Boutell
IPC: C12P19/34 , C12Q1/6874
Abstract: Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.
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公开(公告)号：US10253359B2
公开(公告)日：2019-04-09
申请号：US15173447
申请日：2016-06-03
Applicant: ILLUMINA CAMBRIDGE LIMITED
Inventor： Niall Anthony Gormley , Geoffrey Paul Smith , David Bentley , Roberto Rigatti , Shujun Luo
IPC: C40B40/06 , C12Q1/6855 , C12N15/10 , C12Q1/686
Abstract: The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.
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公开(公告)号：US09977861B2
公开(公告)日：2018-05-22
申请号：US13793676
申请日：2013-03-11
Applicant: ILLUMINA CAMBRIDGE LIMITED
Inventor： Roberto Rigatti , Jonathan Mark Boutell
CPC classification number: G06F19/22 , C12Q1/6869 , C12Q2523/125 , C12Q2525/117
Abstract: The present disclosure provides methods and systems for determining and/or characterizing one or more haplotypes and/or phasing of haplotypes in a nucleic acid sample. In particular, the disclosure provides methods for determining a haplotype and/or phasing of haplotypes in a nucleic acid sample by incorporating synthetic polymorphisms into fragments of a nucleic acid sample and utilizing the synthetic polymorphisms in determining one or more haplotypes and/or phasing of haplotypes.
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公开(公告)号：US20150203910A1
公开(公告)日：2015-07-23
申请号：US14574023
申请日：2014-12-17
Applicant: Illumina Cambridge Limited
Inventor： Geoffrey Paul Smith , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , David Bentley , Tobias William Barr Ost , Vincent Peter Smith , Graham John Worsley , Eric Hans Vermaas
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
Abstract translation: 本发明提供了双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号：US20150197799A1
公开(公告)日：2015-07-16
申请号：US14575863
申请日：2014-12-18
Applicant: Illumina Cambridge Limited
Inventor： Roberto Rigatti , Niall Anthony Gormley , Allen E. Eckhardt , Jonathan Mark Boutell
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6837 , C12Q1/6853 , C12Q1/6869 , C12Q2521/507 , C12Q2535/122 , C12Q2565/543
Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.
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公开(公告)号：US09079148B2
公开(公告)日：2015-07-14
申请号：US14204329
申请日：2014-03-11
Applicant: Illumina Cambridge Limited
Inventor： Roberto Rigatti , Geoffrey Paul Smith , Jonathan Mark Boutell
CPC classification number: C12N15/1068 , B01J19/0046 , C12Q1/6837 , C12Q1/6874 , C12Q2565/543 , C12Q2565/537 , C12Q2563/149 , C12Q2563/161
Abstract: The present invention provides methods for creating an array of features on a surface based on content transferred from a plurality of beads to the surface. Nucleic acid content can be transferred using a method including the steps of (a) providing a surface having one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface.
Abstract translation: 本发明提供了基于从多个珠粒转移到表面的内容来在表面上创建特征阵列的方法。 可以使用包括以下步骤的方法转移核酸含量：（a）提供具有附着于所述表面的一种或多种引物寡核苷酸的表面; （b）提供珠池，其中所述池中的珠子具有连接到其上的多个模板，所述多个模板包含单个核酸模板序列的多个拷贝; （c）通过将模板与引物寡核苷酸杂交将珠子排列在表面上; 和（d）扩展引物以产生附着在表面上的模板的拷贝。
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公开(公告)号：US20140336057A1
公开(公告)日：2014-11-13
申请号：US14281466
申请日：2014-05-19
Applicant: Illumina Cambridge Limited
Inventor： Roberto Rigatti , Tobias William Barr Ost
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q2535/119 , C12Q2535/122 , C12Q2565/513 , C12Q2565/543
Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
Abstract translation: 本发明涉及用于双链多核苷酸模板的成对测序的方法，该方法导致多核苷酸模板的两个不同和分离的区域中核苷酸序列的顺序测定。 使用本发明的方法，可以从聚簇阵列上的每个双链模板获得序列信息的两个链接或配对读取，而不仅仅是从模板的一条链读取的单个测序。
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公开(公告)号：US12071711B2
公开(公告)日：2024-08-27
申请号：US17470434
申请日：2021-09-09
Applicant: Illumina Cambridge Limited
Inventor： Niall Anthony Gormley , Geoffrey Paul Smith , David Bentley , Roberto Rigatti , Shujun Luo
IPC: C40B50/06 , C12N15/10 , C12Q1/6855 , C12Q1/686 , C40B40/06
CPC classification number: C40B50/06 , C12N15/1068 , C12N15/1093 , C12N15/1096 , C12Q1/6855 , C12Q1/686 , C40B40/06 , Y10T436/143333 , C12Q1/6855 , C12Q2565/525 , C12Q2525/313 , C12N15/1093 , C12Q2525/313 , C12Q2565/525
Abstract: The present invention relates to a method for preparing a library of template polynucleotides and use thereof in methods of solid-phase nucleic acid amplification. More specifically, the invention relates to a method for preparing a library of template polynucleotides that have common sequences at their 5′ ends and at their 3′ ends.
