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公开(公告)号：US07943369B2
公开(公告)日：2011-05-17
申请号：US11517090
申请日：2006-09-07
申请人： In Ho Lee , Jun Hong Min , Su Hyeon Kim , Chin Sung Park , Ah gi Kim , Kui Hyun Kim
发明人： In Ho Lee , Jun Hong Min , Su Hyeon Kim , Chin Sung Park , Ah gi Kim , Kui Hyun Kim
IPC分类号： C12M1/34
CPC分类号： G01N33/5308
摘要： A DNA (Deoxyribo Nucleic Acid) detection device and a manufacturing method thereof detects DNA by an electrical method without a separate process for detection by using semiconductor microfabrication techniques. The DNA detection device includes a pair of chambers formed on a semiconductor substrate for accommodating a detection sample, a channel connecting the pair of chambers and a lid covering the pair of chambers. According to the present invention, it is possible to attain a DNA detection device, which can be mass-produced from a silicon substrate by using semiconductor manufacturing technology with improved microfabrication techniques, and a manufacturing method thereof.
摘要翻译： DNA（脱氧核糖核酸）检测装置及其制造方法，通过使用半导体微细加工技术，不用单独的检测方法，通过电气方式检测DNA。 DNA检测装置包括形成在用于容纳检测样品的半导体基板上的一对室，连接该对室的通道和覆盖该对室的盖。 根据本发明，可以通过使用具有改进的微细加工技术的半导体制造技术来获得可以从硅基板批量生产的DNA检测装置及其制造方法。
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2.发明申请公开(公告)号：US20100267011A1
公开(公告)日：2010-10-21
申请号：US11499052
申请日：2006-08-04
申请人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
发明人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
CPC分类号： B82Y30/00 , C12Q1/6816 , C12Q2563/149 , C12Q2525/131 , C12Q2521/514
摘要： There are provided a method and apparatus for detecting nucleic acid using bead and nanopore, and more specifically, a method and apparatus capable of detecting nucleic acid fragments of 70 bps to 300 bps in length by a nanopore detection unit with nanopores of 20 to 120 nm in diameter by attaching a bead to a nucleic acid probe and then detecting the bead attached to nucleic acid not nucleic acid itself. Accordingly, the present invention can detect the nucleic acid fragments using the nanopore detection unit with nanopores of 20 to 120 nm in diameter, even in case where Polymerase Chain Reaction (PCR) products are given as the sample, particularly the PCR products are the nucleic acid fragments of 70 to 300 bps in length.
摘要翻译： 提供了使用珠粒和纳米孔检测核酸的方法和装置，更具体地，可以通过具有20至120nm的纳米孔的纳米孔检测单元来检测长度为70bps至300bps的核酸片段的方法和装置 通过将珠附着到核酸探针上，然后检测附着于核酸而不是核酸本身的珠。 因此，即使在给出聚合酶链反应（PCR）产物作为样品的情况下，本发明也可以使用直径为20-120nm的纳米孔的纳米孔检测单元来检测核酸片段，特别是PCR产物是核酸 长度为70至300 bps的酸性片段。
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公开(公告)号：US08431337B2
公开(公告)日：2013-04-30
申请号：US11499052
申请日：2006-08-04
申请人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
发明人： Kui Hyun Kim , Jun Hong Min , Ah Gi Kim , In Ho Lee
CPC分类号： B82Y30/00 , C12Q1/6816 , C12Q2563/149 , C12Q2525/131 , C12Q2521/514
摘要： There are provided a method and apparatus for detecting nucleic acid using bead and nanopore, and more specifically, a method and apparatus capable of detecting nucleic acid fragments of 70 bps to 300 bps in length by a nanopore detection unit with nanopores of 20 to 120 nm in diameter by attaching a bead to a nucleic acid probe and then detecting the bead attached to nucleic acid not nucleic acid itself. Accordingly, the present invention can detect the nucleic acid fragments using the nanopore detection unit with nanopores of 20 to 120 nm in diameter, even in case where Polymerase Chain Reaction (PCR) products are given as the sample, particularly the PCR products are the nucleic acid fragments of 70 to 300 bps in length.
摘要翻译： 提供了使用珠粒和纳米孔检测核酸的方法和装置，更具体地，可以通过具有20至120nm的纳米孔的纳米孔检测单元来检测长度为70bps至300bps的核酸片段的方法和装置 通过将珠附着到核酸探针上，然后检测附着于核酸而不是核酸本身的珠。 因此，即使在给出聚合酶链反应（PCR）产物作为样品的情况下，本发明也可以使用直径为20-120nm的纳米孔的纳米孔检测单元来检测核酸片段，特别是PCR产物是核酸 长度为70至300 bps的酸性片段。
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4.发明申请公开(公告)号：US20070218471A1
公开(公告)日：2007-09-20
申请号：US11550099
申请日：2006-10-17
申请人： Young Rok Kim , Jun Hong Min , In Ho Lee , Ah gi Kim , Kui Hyun Kim
发明人： Young Rok Kim , Jun Hong Min , In Ho Lee , Ah gi Kim , Kui Hyun Kim
CPC分类号： G01N33/48721 , C12Q1/6825 , C12Q2565/631
摘要： Disclosed herein is a method for detecting DNA using a nanopore including treating the surface of a nanopore formed in a solid substrate with a substance carrying positive charges; introducing a DNA-containing sample into the surface-treated nanopore; and detecting electrical signals generated during translocation of the sample through the nanopore. Also disclosed herein is device for detecting DNA using a nanopore including a solid substrate including a nanopore, treated with a substance which carries positive charges to change a surface property of the nanopore so that the nanopore surface carries positive charges; an electrode applying voltage to the nanopore of the solid substrate; and a measurement unit measuring an electrical signal generated during translocation of a DNA-containing sample through the nanopore.
摘要翻译： 本文公开了一种使用纳米孔检测DNA的方法，包括用携带正电荷的物质处理在固体基质中形成的纳米孔的表面; 将含DNA样品引入表面处理的纳米孔中; 并检测通过纳米孔在样品易位期间产生的电信号。 本文还公开了使用纳米孔检测DNA的装置，所述纳米孔包括包含纳米孔的固体基质，用携带正电荷的物质处理以改变纳米孔的表面性质，使得纳米孔表面带有正电荷; 电极向固体基底的纳米孔施加电压; 以及测量单元，其测量通过纳米孔在含DNA样品的易位期间产生的电信号。
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5.发明申请BIOMEMORY DEVICE COMPRISING HETEROLAYER OF RECOMBINANT PROTEIN AND INORGANIC PARTICLE 有权包含重组蛋白和无机颗粒的杂环化合物的生物装置公开(公告)号：US20130157335A1
公开(公告)日：2013-06-20
申请号：US13564136
申请日：2012-08-01
申请人： Jeong Woo Choi , Taek Lee , Jun Hong Min
发明人： Jeong Woo Choi , Taek Lee , Jun Hong Min
摘要： The present invention relates to a biomemory device, comprising (a) a substrate; and (b) a heterolayer comprising a protein having a redox potential and an inorganic particle; wherein the heterolayer is immobilized on the substrate. By applying inorganic particles, the present invention provides a biomemory device capable of enhancing low current signals detected electron transfer between biomolecules and substrates up to at least five (5) times greater signals. The present invention is capable of controlling the redox states with help of redox potentials of proteins depending on applied potential. The present invention provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule.
摘要翻译： 本发明涉及一种生物记忆装置，包括（a）基底; 和（b）包含具有氧化还原电位的蛋白质和无机颗粒的分层; 其中所述异层固定在所述基底上。 通过施加无机颗粒，本发明提供了一种生物记录装置，其能够增强在至少五（5）倍的信号下检测到生物分子和基板之间的电子转移的低电流信号。 本发明能够根据应用的潜力帮助蛋白质的氧化还原电位来控制氧化还原状态。 本发明提供了一种基于天然存在的生物分子的电子转移原理的作为信息存储装置的新概念的生物记忆装置。
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公开(公告)号：US20130029396A1
公开(公告)日：2013-01-31
申请号：US13404696
申请日：2012-02-24
申请人： Jeong Woo Choi , Taek Lee , Jun Hong Min
发明人： Jeong Woo Choi , Taek Lee , Jun Hong Min
IPC分类号： C12N11/16 , C07K14/825 , C07K14/00
CPC分类号： C07K14/00 , C12N9/0004 , C12N11/00 , C12N11/14 , C12Y120/09001
摘要： The present disclosure relates to a multifunctional biomemory device in which a protein having a redox potential substituted with a metal ion is directly immobilized on a substrate. The present disclosure provides an operating method in which the redox state of the protein is controlled by applying three different potentials. The present disclosure provides a biomemory device in which the metal ion of a metalloprotein is substituted to allow for artificial control of the redox potential. The present disclosure provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule.
摘要翻译： 本公开内容涉及一种多功能生物记录装置，其中具有被金属离子取代的氧化还原电位的蛋白质直接固定在基底上。 本公开提供了通过应用三种不同电位来控制蛋白质的氧化还原状态的操作方法。 本公开提供了其中金属蛋白的金属离子被替代以允许人为控制氧化还原电位的生物记忆装置。 本公开提供了一种基于天然存在的生物分子的电子转移原理的作为信息存储装置的新概念生物记忆装置。
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公开(公告)号：US08569008B2
公开(公告)日：2013-10-29
申请号：US13404696
申请日：2012-02-24
申请人： Jeong Woo Choi , Taek Lee , Jun Hong Min
发明人： Jeong Woo Choi , Taek Lee , Jun Hong Min
IPC分类号： C12Q1/26
CPC分类号： C07K14/00 , C12N9/0004 , C12N11/00 , C12N11/14 , C12Y120/09001
摘要： The present disclosure relates to a multifunctional biomemory device in which a protein having a redox potential substituted with a metal ion is directly immobilized on a substrate. The present disclosure provides an operating method in which the redox state of the protein is controlled by applying three different potentials. The present disclosure provides a biomemory device in which the metal ion of a metalloprotein is substituted to allow for artificial control of the redox potential. The present disclosure provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule.
摘要翻译： 本公开内容涉及一种多功能生物记录装置，其中具有被金属离子取代的氧化还原电位的蛋白质直接固定在基底上。 本公开提供了通过应用三种不同电位来控制蛋白质的氧化还原状态的操作方法。 本公开提供了其中金属蛋白的金属离子被替代以允许人为控制氧化还原电位的生物记忆装置。 本公开提供了一种基于天然存在的生物分子的电子转移原理的作为信息存储装置的新概念生物记忆装置。
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公开(公告)号：US09796789B2
公开(公告)日：2017-10-24
申请号：US13564136
申请日：2012-08-01
申请人： Jeong Woo Choi , Taek Lee , Jun Hong Min
发明人： Jeong Woo Choi , Taek Lee , Jun Hong Min
摘要： The present invention relates to a biomemory device, comprising (a) a substrate; and (b) a heterolayer comprising a protein having a redox potential and an inorganic particle; wherein the heterolayer is immobilized on the substrate. By applying inorganic particles, the present invention provides a biomemory device capable of enhancing low current signals detected electron transfer between biomolecules and substrates up to at least five (5) times greater signals. The present invention is capable of controlling the redox states with help of redox potentials of proteins depending on applied potential. The present invention provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule.
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