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公开(公告)号：US08334429B2
公开(公告)日：2012-12-18
申请号：US12650106
申请日：2009-12-30
CPC分类号： C12N15/8205 , C12N9/1007
摘要： Auxotrophic Agrobacterium and methods employing auxotrophic Agrobacterium are provided. Auxotrophic Agrobacterium may be used in a variety of methods including biologically containing Agrobacterium comprising a transgene and transforming a plant cell without using an Agrobacterium counter-selective agent. Transforming maize immature embryos using an Agrobacterium auxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic Agrobacterium. Methods for producing an Agrobacterium thymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.
摘要翻译： 营养缺陷型农杆菌和使用营养缺陷型农杆菌的方法。 营养不良农杆菌可以以多种方法使用，包括生物地含有转基因的农杆菌，并且不使用农杆菌反选择剂来转化植物细胞。 使用农杆菌营养缺陷型用于胸苷转化玉米未成熟胚，导致与使用原养性农杆菌的转化相当的转化效率。 公开了用于生产农杆菌营养缺陷型农杆菌和在转化方法中使用营养缺陷型的方法。 还提供了使用本发明的方法生产的转化的组织和植物。
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2.发明授权UDP-xylose synthases (UXS) polynucleotides, polypeptides and uses thereof 有权UDP-木糖合酶（UXS）多核苷酸，多肽及其用途公开(公告)号：US08735652B2
公开(公告)日：2014-05-27
申请号：US13614800
申请日：2012-09-13
申请人： Xiaoming Bao , George W Singletary , Deborah J Wetterberg , Ramesh Nair , Kanwarpal S Dhugga , Matthias Liebergesell , David A Selinger
发明人： Xiaoming Bao , George W Singletary , Deborah J Wetterberg , Ramesh Nair , Kanwarpal S Dhugga , Matthias Liebergesell , David A Selinger
CPC分类号： C12N9/88 , C12N15/8245 , C12N15/8246 , C12N15/8261 , C12Y401/01035 , Y02A40/146
摘要： The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (USX) genes.
摘要翻译： 本公开涉及与UXS多肽和/或编码UXS多肽的核酸相关的方法和组合物。 在某些权利要求中，所述方法和组合物可用于改善消化率和/或颗粒加工的易于使用。 这种改进涉及转基因植物中阿拉伯木聚糖和/或半纤维素含量的调节。 这样的植物可以例如包含抑制一种或多种UDP-木糖合酶（USX）基因表达的一种或多种核酸序列。
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公开(公告)号：US07341856B2
公开(公告)日：2008-03-11
申请号：US11487811
申请日：2006-07-17
申请人： Kanwarpal S. Dhugga , Chun Ping Li , Jian G. Dong , William D. Hitz , Matthias Liebergesell , Scott E. Nichols , Kristen K. Briggs
发明人： Kanwarpal S. Dhugga , Chun Ping Li , Jian G. Dong , William D. Hitz , Matthias Liebergesell , Scott E. Nichols , Kristen K. Briggs
CPC分类号： C12N9/0006 , C12N9/00 , C12N9/88 , C12N15/52 , C12N15/8243 , C12N15/8247 , C12P7/625
摘要： The invention relates to the genetic manipulation of plants to produce polyhydroxyalkanoate, particularly in the peroxisomes. Methods for producing such polymers in plants and host cells are provided. Such methods find use in producing biodegradable thermoplastics in plants and other organisms. Nucleotide molecules, expression cassettes, and genetically manipulated host cell, plants, plant tissues, and seeds are also provided.
摘要翻译： 本发明涉及植物生产聚羟基链烷酸酯的遗传操作，特别是在过氧化物酶体中。 提供了在植物和宿主细胞中生产这种聚合物的方法。 这种方法可用于在植物和其他生物体中生产可生物降解的热塑性塑料。 还提供了核苷酸分子，表达盒和遗传操纵的宿主细胞，植物，植物组织和种子。
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公开(公告)号：US20060253937A1
公开(公告)日：2006-11-09
申请号：US11487718
申请日：2006-07-17
申请人： Kanwarpal Dhugga , Chun Li , Jian Dong , William Hitz , Matthias Liebergesell , Scott Nichols , Kristen Briggs
发明人： Kanwarpal Dhugga , Chun Li , Jian Dong , William Hitz , Matthias Liebergesell , Scott Nichols , Kristen Briggs
CPC分类号： C12N15/8243
摘要： The invention relates to the genetic manipulation of plants to produce polyhydroxyalkanoate, particularly in the peroxisomes. Methods for producing such polymers in plants and host cells are provided. Such methods find use in producing biodegradable thermoplastics in plants and other organisms. Nucleotide molecules, expression cassettes, and genetically manipulated host cell, plants, plant tissues, and seeds are also provided.
摘要翻译： 本发明涉及植物生产聚羟基链烷酸酯的遗传操作，特别是在过氧化物酶体中。 提供了在植物和宿主细胞中生产这种聚合物的方法。 这种方法可用于在植物和其他生物体中生产可生物降解的热塑性塑料。 还提供了核苷酸分子，表达盒和遗传操纵的宿主细胞，植物，植物组织和种子。
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公开(公告)号：US20080320613A1
公开(公告)日：2008-12-25
申请号：US12185542
申请日：2008-08-04
IPC分类号： C12N15/11
CPC分类号： C12N9/0069 , C12N15/8245 , C12N15/8246 , C12N15/8261 , C12N15/8267 , Y02A40/146
摘要： Compositions and methods for modulating MIOX activity are provided. Such compositions include nucleotide sequences for novel MIOX sequences obtained from maize, amino acid sequences for the proteins encoded by the nucleotide sequences of the invention, and variants and fragments thereof. Methods of the invention involve introducing into a plant a nucleotide construct comprising a MIOX nucleotide sequence operably linked to a promoter that drives expression in a plant. Expression of the novel nucleotide sequences disclosed herein confers advantageous agronomic properties on a plant. Transformed plants, plant cells, and seeds are additionally provided.
摘要翻译： 提供了调节MIOX活性的组合物和方法。 这样的组合物包括从玉米获得的新型MIOX序列的核苷酸序列，由本发明的核苷酸序列编码的蛋白质的氨基酸序列及其变体和片段。 本发明的方法包括向植物中引入一种核苷酸构建体，该核苷酸构建体包含可操作地连接到驱动植物表达的启动子的MIOX核苷酸序列。 本文公开的新型核苷酸序列的表达赋予植物有利的农学性质。 另外提供转化的植物，植物细胞和种子。
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公开(公告)号：US20060260006A1
公开(公告)日：2006-11-16
申请号：US11487852
申请日：2006-07-17
申请人： Kanwarpal Dhugga , Chun Li , Jian Dong , William Hitz , Matthias Liebergesell , Scott Nichols , Kristen Briggs
发明人： Kanwarpal Dhugga , Chun Li , Jian Dong , William Hitz , Matthias Liebergesell , Scott Nichols , Kristen Briggs
CPC分类号： C12N15/8243
摘要： The invention relates to the genetic manipulation of plants to produce polyhydroxyalkanoate, particularly in the peroxisomes. Methods for producing such polymers in plants and host cells are provided. Such methods find use in producing biodegradable thermoplastics in plants and other organisms. Nucleotide molecules, expression cassettes, and genetically manipulated host cell, plants, plant tissues, and seeds are also provided.
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7.发明授权公开(公告)号：US06515205B1
公开(公告)日：2003-02-04
申请号：US08178257
申请日：1994-05-06
IPC分类号： C12N1582
CPC分类号： C12N9/0006 , C12N9/1029 , C12N15/52 , C12N15/8243 , C12P7/625
摘要： Genes encoding polyhydroxyalkanoate synthase, &bgr;-ketothiolase and acetoacetyl CoA reductase are isolated from the publicly available bacterium Chromatium vinosum. Recombinant genomes of plants or other species of bacteria which contain these genes are capable of producing polyalkanoate polymers. The nucleotide sequences of the said three genees have been determined.
摘要翻译： 编码聚羟基链烷酸酯合成酶，β-酮硫解酶和乙酰乙酰辅酶A还原酶的基因从公开获得的细菌色氨酸杆菌分离。 含有这些基因的植物或其他细菌种类的重组基因组能够生产聚链烷酸酯聚合物。 已经确定了所述三种基因的核苷酸序列。
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8.发明申请UDP-XYLOSE SYNTHASES (UXS) POLYNUCLEOTIDES, POLYPEPTIDES AND USES THEREOF 有权UDP-XYLOSE SYNTHASES（UXS）多核苷酸，多聚体及其用途公开(公告)号：US20130014290A1
公开(公告)日：2013-01-10
申请号：US13614800
申请日：2012-09-13
申请人： Xiaoming Bao , George W. Singletary , Deborah J. Wetterberg , Ramesh Nair , Kanwarpal S. Dhugga , Matthias Liebergesell , David A. Selinger
发明人： Xiaoming Bao , George W. Singletary , Deborah J. Wetterberg , Ramesh Nair , Kanwarpal S. Dhugga , Matthias Liebergesell , David A. Selinger
CPC分类号： C12N9/88 , C12N15/8245 , C12N15/8246 , C12N15/8261 , C12Y401/01035 , Y02A40/146
摘要： The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (USX) genes.
摘要翻译： 本公开涉及与UXS多肽和/或编码UXS多肽的核酸相关的方法和组合物。 在某些权利要求中，所述方法和组合物可用于改善消化率和/或颗粒加工的易于使用。 这种改进涉及转基因植物中阿拉伯木聚糖和/或半纤维素含量的调节。 这样的植物可以例如包含抑制一种或多种UDP-木糖合酶（USX）基因表达的一种或多种核酸序列。
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9.发明授权UDP-xylose synthases (UXS) polynucleotides, polypeptides, and uses thereof 失效UDP-木糖合酶（UXS）多核苷酸，多肽及其用途公开(公告)号：US07592505B2
公开(公告)日：2009-09-22
申请号：US11614098
申请日：2006-12-21
申请人： Xiaoming Bao , George W. Singletary , Deborah J. Wetterberg , Ramesh Nair , Kanwarpal S. Dhugga , Matthias Liebergesell , David A. Selinger
发明人： Xiaoming Bao , George W. Singletary , Deborah J. Wetterberg , Ramesh Nair , Kanwarpal S. Dhugga , Matthias Liebergesell , David A. Selinger
CPC分类号： C12N9/88 , C12N15/8245 , C12N15/8246 , C12N15/8261 , C12Y401/01035 , Y02A40/146
摘要： The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (UXS) genes.
摘要翻译： 本公开涉及与UXS多肽和/或编码UXS多肽的核酸相关的方法和组合物。 在某些权利要求中，所述方法和组合物可用于改善消化率和/或颗粒加工的易于使用。 这种改进涉及转基因植物中阿拉伯木聚糖和/或半纤维素含量的调节。 这样的植物可以例如包含抑制一种或多种UDP-木糖合酶（UXS）基因表达的一种或多种核酸序列。
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10.发明申请UDP-Xylose Synthases (UXS) Polynucleotides, Polypeptides, and Uses Thereof 失效UDP-木糖合酶（UXS）多核苷酸，多肽及其用途公开(公告)号：US20070199105A1
公开(公告)日：2007-08-23
申请号：US11614098
申请日：2006-12-21
申请人： Xiaoming Bao , George Singletary , Deborah Wetterberg , Ramesh Nair , Kanwarpal Dhugga , Matthias Liebergesell , David Selinger
发明人： Xiaoming Bao , George Singletary , Deborah Wetterberg , Ramesh Nair , Kanwarpal Dhugga , Matthias Liebergesell , David Selinger
CPC分类号： C12N9/88 , C12N15/8245 , C12N15/8246 , C12N15/8261 , C12Y401/01035 , Y02A40/146
摘要： The present disclosure concerns methods and compositions relating to UXS polypeptides and/or nucleic acids encoding UXS polypeptides. In certain claims, the methods and compositions are of use to improve digestibility and/or ease of grain processing. Such improvements relate to a modulation in arabinoxylan and/or hemicellulose content in transgenic plants. Such plants can, for example, comprise one or more nucleic acid sequences that inhibit expression of one or more UDP-Xylose Synthase (UXS) genes.
摘要翻译： 本公开涉及与UXS多肽和/或编码UXS多肽的核酸相关的方法和组合物。 在某些权利要求中，所述方法和组合物可用于改善消化率和/或颗粒加工的易于使用。 这种改进涉及转基因植物中阿拉伯木聚糖和/或半纤维素含量的调节。 这样的植物可以例如包含抑制一种或多种UDP-木糖合酶（UXS）基因表达的一种或多种核酸序列。
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