公开(公告)号：US20060263257A1

公开(公告)日：2006-11-23

申请号：US11302733

申请日：2005-12-13

申请人： Jesse Beauchamp ,  Robert Hodyss ,  Karin Oberg

发明人： Jesse Beauchamp ,  Robert Hodyss ,  Karin Oberg

IPC分类号： B32B27/04 ,  G01N30/96

CPC分类号： G01N21/80 ,  G01N21/783 ,  G01N2021/7793 ,  G01N2201/08

摘要： A new optical sensing method for detection of analyte vapors down to ppb levels is described. The sensor is based on the use of a visible indicator, such as Bromocresol green, adsorbed onto a high surface area substrate, such as a silica sphere matrix. When the analyte gas is adsorb onto the matrix, the indicator undergoes a color change. The color change in turn is detected with a suitable spectrometer. Sensor performance is demonstrated for an exemplary amine sensor for the aliphatic amines tert-butylamine, diethylamine and triethylamine and also for pyridine and aniline. The microsphere sensor is more sensitive than other prior art optical amine sensor designs. The sensor response varies with temperature, with lower sensitivity and faster response at higher temperatures allowing for adjustment to prioritize sensitivity or speed. The sensor response is also highly reproducible and fully reversible.

摘要翻译： 描述了用于检测低至ppb水平的分析物蒸气的新的光学感测方法。 传感器基于使用诸如溴甲酚绿的可见指示剂，其被吸附到诸如二氧化硅球体基质的高表面积底物上。 当分析物气体吸附在基质上时，指示剂发生颜色变化。 用合适的光谱仪检测颜色变化。 针对脂族胺叔丁胺，二乙胺和三乙胺以及吡啶和苯胺的示例性胺传感器证明了传感器性能。 微球传感器比其它现有技术的光学胺传感器设计更加敏感。 传感器响应随温度而变化，灵敏度较低，响应速度更快，可调整灵敏度或速度优先级。 传感器响应也是高度可重复的并且完全可逆。

公开(公告)号：US20070087447A1

公开(公告)日：2007-04-19

申请号：US11474028

申请日：2006-06-23

申请人： Jesse Beauchamp ,  Robert Hodyss ,  Heather Sumner

发明人： Jesse Beauchamp ,  Robert Hodyss ,  Heather Sumner

IPC分类号： G01N24/00

CPC分类号： G01N33/6848 ,  Y10T436/24 ,  Y10T436/25375

摘要： A method for the selective fragmentation of peptides using free radical initiator-peptide conjugates is provided. Free radical initiated peptide sequencing, or FRIPS, consists of covalently attaching a free radical initiator to a peptide and collisionally activating this conjugate. This results in fragment formation. Subsequent collision-activated dissociation further dissociates the fragments, producing a group of ions based on the interaction of the free radical initiator and the target molecule. The methodology is applied to the fundamental study of biologically relevant reactions of free radicals with peptides and proteins in the gas phase, as well as to a completely gas-phase approach to protein sequencing.

摘要翻译： 提供了使用自由基引发剂 - 肽缀合物选择性断裂肽的方法。 自由基引发的肽测序或FRIPS由将自由基引发剂共价连接到肽和碰撞激活该缀合物组成。 这导致片段形成。 随后的碰撞激活解离进一步解离片段，基于自由基引发剂和靶分子的相互作用产生一组离子。 该方法应用于气相中自由基与肽和蛋白质的生物相关反应的基础研究，以及完全气相方法的蛋白质测序。

公开(公告)号：US20050010059A1

公开(公告)日：2005-01-13

申请号：US10782373

申请日：2004-02-18

申请人： Jesse Beauchamp ,  Ryan Julian ,  Brian Stoltz ,  Jeremy May

发明人： Jesse Beauchamp ,  Ryan Julian ,  Brian Stoltz ,  Jeremy May

IPC分类号： C07K1/08 ,  C07F1/08

CPC分类号： C07K1/088

摘要： Biomimetic reagents capable of selectively forming non-covalent complexes and initiating intermolecular reactions with peptides in the gas phase are described. The reagents are particularly useful in gas phase peptides chemistry.

摘要翻译： 描述了能够选择性地形成非共价复合物并且引发与气相中的肽的分子间反应的生物模拟试剂。 试剂在气相肽化学中特别有用。

公开(公告)号：US20060249672A1

公开(公告)日：2006-11-09

申请号：US11390695

申请日：2006-03-28

申请人： Ronald Grimm ,  Jesse Beauchamp

发明人： Ronald Grimm ,  Jesse Beauchamp

IPC分类号： H01J49/00

CPC分类号： H01J49/165

摘要： A method and apparatus for probing the chemistry of a single droplet are provided. The technique uses a variation of the field-induced droplet ionization (FIDI) method, in which isolated droplets undergo heterogeneous reactions between solution phase analytes and gas-phase species. Following a specified reaction time, the application of a high electric field induces FIDI in the droplet, generating fine jets of highly charged progeny droplets that can then be characterized. Sampling over a range of delay times following exposure of the droplet to gas phase reactants, the spectra yield the temporal variation of reactant and product concentrations. Following the initial mass spectrometry studies, we developed an experiment to explore the parameter space associated with FIDI in an attempt to better understand and control the technique. In an alternative embodiment of the invention switched electric fields are integrated with the technique to allow for time-resolved studies of the droplet distortion, jetting, and charged progeny droplet formation associated with FIDI.

摘要翻译： 提供了用于探测单个液滴的化学的方法和装置。 该技术使用场诱导的液滴电离（FIDI）方法的变化，其中分离的液滴在溶液相分析物和气相物质之间发生非均相反应。 在指定的反应时间之后，高电场的应用在液滴中诱导FIDI，产生高度带电的后代液滴的细小射流，然后可以进行表征。 在将液滴暴露于气相反应物后的延迟时间范围内取样，光谱产生反应物和产物浓度的时间变化。 在最初的质谱研究之后，我们开发了一个实验来探索与FIDI相关的参数空间，从而更好地理解和控制该技术。 在本发明的替代实施例中，切换电场与该技术集成，以允许对与FIDI相关联的液滴变形，喷射和带电后代液滴形成的时间分辨研究。