公开(公告)号：US07659388B2

公开(公告)日：2010-02-09

申请号：US11862758

申请日：2007-09-27

申请人： Ji-young Oh ,  Sang-hyun Paek ,  Kyung-hee Park ,  Jung-nam Lee ,  Jong-suk Chung ,  Ah-gi Kim

发明人： Ji-young Oh ,  Sang-hyun Paek ,  Kyung-hee Park ,  Jung-nam Lee ,  Jong-suk Chung ,  Ah-gi Kim

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q2600/16 ,  Y02A50/451

摘要： Provided are a primer set for specifically amplifying target sequence(s) of twelve respiratory disease-causing bacterial species, a probe set specifically hybridizing with target sequence(s) of twelve respiratory disease-causing bacterial species, a microarray comprising the probe set, and a method of detecting a respiratory disease-causing bacterial species using the probe set.

摘要翻译： 提供了用于特异性扩增十二种呼吸道性疾病的细菌种类的靶序列的引物组，与12种呼吸道性疾病致细菌物种的靶序列特异性杂交的探针组，包含探针组的微阵列和 使用探针组检测呼吸系统疾病的细菌物种的方法。

公开(公告)号：US20090169431A1

公开(公告)日：2009-07-02

申请号：US12364235

申请日：2009-02-02

申请人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

发明人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

IPC分类号： G01N27/00

CPC分类号： B82Y5/00 ,  C12Q1/6825 ,  Y10S436/806 ,  Y10T436/143333 ,  C12Q2565/631

摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.

摘要翻译： 提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。 可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。

公开(公告)号：US07504261B2

公开(公告)日：2009-03-17

申请号：US11414022

申请日：2006-04-28

申请人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

发明人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

IPC分类号： G01N33/00 ,  G01N33/53 ,  C07H21/04

CPC分类号： B82Y5/00 ,  C12Q1/6825 ,  Y10S436/806 ,  Y10T436/143333 ,  C12Q2565/631

摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.

摘要翻译： 提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。 可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。

公开(公告)号：US20060292605A1

公开(公告)日：2006-12-28

申请号：US11414022

申请日：2006-04-28

申请人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

发明人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： B82Y5/00 ,  C12Q1/6825 ,  Y10S436/806 ,  Y10T436/143333 ,  C12Q2565/631

摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.