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31 条记录 (耗时 0.018 秒)

    Method of purifying ferment-produced riboflavin
    1.
    发明授权
    Method of purifying ferment-produced riboflavin 失效
    净化发酵的RIBOFLAVIN的方法

    公开(公告)号：US5210023A

    公开(公告)日：1993-05-11

    申请号：US724056

    申请日：1991-07-01

    申请人： Johannes Grimmer ,  Hans Kiefer ,  Christoph Martin

    发明人： Johannes Grimmer ,  Hans Kiefer ,  Christoph Martin

    IPC分类号： C07D475/14 ,  C12P17/18

    CPC分类号： C07D475/14

    摘要： A method of purifying ferment-produced riboflavin, wherein the impure riboflavin is suspended in water or dilute aqueous acid, and the suspension is heated at a temperature of from 75.degree. to 130.degree. C. for from 0.3 to 3 hours with stirring, during which time crystal transformation takes place, after which the mixture is cooled and the purified product is isolated in known manner.

    摘要翻译： 一种纯化发酵生产的核黄素的方法，其中不纯的核黄素悬浮在水中或稀释酸水溶液，悬浮液在75-130℃的温度下搅拌加热0.3-3小时，在此期间 发生时间晶体转变，然后将混合物冷却，以已知方式分离纯化产物。

    Preparation of riboflavin 5'-phosphate (FMN) and its sodium salt in a lactone
    5.
    发明授权
    Preparation of riboflavin 5'-phosphate (FMN) and its sodium salt in a lactone 失效
    五磷酸五磷酸（FMN）及其盐中的盐酸盐的制备

    公开(公告)号：US5095115A

    公开(公告)日：1992-03-10

    申请号：US571230

    申请日：1990-08-23

    申请人： Johannes Grimmer ,  Hans Kiefer

    发明人： Johannes Grimmer ,  Hans Kiefer

    IPC分类号： A61K31/675 ,  A61P3/02 ,  C07F9/6524 ,  C07F9/6561 ,  C07F9/6574

    CPC分类号： C07F9/65618 ,  C07F9/65742

    摘要： An improved process for preparing riboflavin 5'-phosphate (FMN) or its monosodium salt by reacting riboflavin with excess phosphorus oxychloride in a solvent, hydrolyzing of the resulting reaction mixture and, if desired, partially neutralizing with aqueous sodium hydroxide solution, comprises reacting the riboflavin with from 1.2 to 3 moles of phosphorus oxychloride per mole of riboflavin in a suitable lactone, especially .gamma.-butyrolactone, as solvent. The process is particularly advantageous when the reaction mixture is hydrolyzed at from 80.degree. to 90.degree. C. continuously or batchwise.

    摘要翻译： 通过使核黄素与过量的三氯氧化磷在溶剂中反应制备核黄素5'-磷酸（FMN）或其单钠盐的改进方法，水解所得反应混合物，如果需要，用氢氧化钠水溶液部分中和， 在适当的内酯，特别是γ-丁内酯中，每摩尔核黄素使用1.2至3摩尔磷酰氯的核黄素作为溶剂。 当反应混合物连续或间歇地在80℃至90℃下水解时，该方法特别有利。

    Refolding of membrane proteins
    6.
    发明申请
    Refolding of membrane proteins 审中-公开
    膜蛋白重折叠

    公开(公告)号：US20060058509A1

    公开(公告)日：2006-03-16

    申请号：US11217124

    申请日：2005-08-31

    申请人： Hans Kiefer ,  Klaus Maier

    发明人： Hans Kiefer ,  Klaus Maier

    IPC分类号： C07K14/47

    CPC分类号： C07K1/1136

    摘要： In a method for production of membrane receptors folded into their native structure, first, receptors solubilized in a first detergent are provided. To induce folding of receptors into their native form, the first detergent is exchanged for a second detergent. Both for the first and for the second detergent, examples are shown.

    摘要翻译： 在制备折叠成其天然结构的膜受体的方法中，首先提供溶解在第一种洗涤剂中的受体。 为了诱导受体折叠成天然形式，将第一种洗涤剂换成第二种洗涤剂。 对于第一和第二种洗涤剂都是示出的。

    Refolding of ion channel proteins
    7.
    发明申请
    Refolding of ion channel proteins 审中-公开
    离子通道蛋白重折叠

    公开(公告)号：US20050255556A1

    公开(公告)日：2005-11-17

    申请号：US11102487

    申请日：2005-04-08

    申请人： Dietrich Loebel ,  Tilmann Roos ,  Klaus Maier ,  Hans Kiefer

    发明人： Dietrich Loebel ,  Tilmann Roos ,  Klaus Maier ,  Hans Kiefer

    IPC分类号： C07K1/113 ,  C07K14/705 ,  C07H21/04 ,  C12N15/09 ,  C12P21/06 ,  C12Q1/68

    CPC分类号： C07K1/1136 ,  C07K14/705

    摘要： A method of ion channels folded into their active structure, or functional subunits thereof, is described. For this purpose, initially expressed subunits, which are solubilized and denatured in a first detergent, of an ion channel are provided, and subsequently the first detergent is replaced by a second detergent which induces folding of the subunits of an ion channel into their native structure. The subunits of the ion channel are then assembled into its active structure.

    摘要翻译： 描述了折叠成其活性结构的离子通道的方法或其功能性亚单位。 为此目的，提供了初始表达的在第一洗涤剂中溶解和变性的亚基，其具有离子通道，随后第一种洗涤剂被第二种洗涤剂代替，第二种洗涤剂诱导将离子通道的亚基折叠成其天然结构 。 然后将离子通道的亚基组装成其活性结构。

    Production of N-(1-alkenyl)-carbamyl chlorides
    8.
    发明授权
    Production of N-(1-alkenyl)-carbamyl chlorides 失效
    N-（1-烷基） - 氨基甲酰氯的生产

    公开(公告)号：US4038311A

    公开(公告)日：1977-07-26

    申请号：US533143

    申请日：1974-12-16

    申请人： Hans Kiefer ,  Herbert Naarmann

    发明人： Hans Kiefer ,  Herbert Naarmann

    IPC分类号： C07C271/02 ,  C07C271/06 ,  C08F26/02 ,  C08F226/02 ,  E04H17/14 ,  C07C125/03

    CPC分类号： C08F226/02 ,  C07C271/02 ,  C07C271/06 ,  C08F26/02 ,  E04H17/14

    摘要： The production of N-(1-alkenyl)-carbamyl chlorides by reaction of a Schiff base with phosgene, and the new N-(1-alkenyl)-carbamyl chlorides themselves. The new compounds which can be prepared by the process of the invention are valuable starting materials for the production of coating intermediates, plastics, paints and plant protection agents.One preferred group of compounds available from said process are the N-vinyl compounds of the formula ##STR1## in which R.sup.1 is cyclohexyl, cyclooctyl, norbornyl, tetrahydrodicyclopentadienyl, n-hexyl, n-octyl, 2-ethylhexyl or phenyl. Another preferred group of compounds are the N-vinyl-N-t-alkylcarbamyl chlorides of 4 to 6 carbon atoms in the tertiary alkyl group, especially N-vinyl-N-t-butylcarbamyl chloride.

    Method of determining radiation doses by evaluating nuclear tracks in irradiated and subsequently etched foils
    10.
    发明授权
    Method of determining radiation doses by evaluating nuclear tracks in irradiated and subsequently etched foils 失效
    通过评估辐射和随后蚀刻的箔中的核轨迹来确定辐射剂量的方法

    公开(公告)号：US4933564A

    公开(公告)日：1990-06-12

    申请号：US356792

    申请日：1989-05-25

    申请人： Hans Kiefer ,  Bernd Reinhardt ,  Gotthard Schleicher ,  Manfred Urban

    发明人： Hans Kiefer ,  Bernd Reinhardt ,  Gotthard Schleicher ,  Manfred Urban

    IPC分类号： G01T5/02 ,  G01T5/10

    CPC分类号： G01T5/10

    摘要： A method of determining radiation doses by evaluation of nuclear tracks in an irradiated foil of track recording material through the use of an electrochemical etching cell which is subdivided into two chambers by the foil with the irradiated side of the foil contacted by a mixture of an etchant and a solvent and the opposite side of the foil contacted by an electrolyte and with a first electrode immersed into the mixture and a second immersed into the electrolyte, the method including the steps of: (a) applying an alternating voltage across the two electrodes establishing an electric field through the foil; (b) etching the nuclear tracks by selective exposure to the mixture for a given period of time in the presence of the electric field; (c) continuously recording the electrical capacitance between the electrodes during the performance of step (b); and (d) comparing the electrical capacitance reached after a given etching time with a calibrated value for obtaining a measure for the radiation dose.

    摘要翻译： 通过使用电化学蚀刻电池评估轨道记录材料的照射箔中的核轨道来确定辐射剂量的方法，所述电化学蚀刻电池被箔与被蚀刻剂的混合物接触的箔的照射侧细分成两个室 和溶剂，并且箔的与电解质接触的相对侧和浸入混合物中的第一电极，并且第二电极浸入电解质中，该方法包括以下步骤：（a）跨两个电极施加交流电压，建立 通过箔片的电场; （b）在存在电场的情况下通过选择性地暴露于混合物一段给定的时间来蚀刻核轨道; （c）在步骤（b）的执行期间连续记录电极之间的电容; 和（d）将给定蚀刻时间之后达到的电容与校准值进行比较，以获得辐射剂量的量度。