公开(公告)号：US20140287957A1

公开(公告)日：2014-09-25

申请号：US13704498

申请日：2011-06-17

Applicant: Stanley B. Prusiner ,  John C. Price ,  Sina Ghaemmaghami ,  Al Burlingame ,  Shenheng Guan

Inventor： Stanley B. Prusiner ,  John C. Price ,  Sina Ghaemmaghami ,  Al Burlingame ,  Shenheng Guan

IPC: G01N33/68 ,  G01N33/50

CPC classification number: G01N33/5088 ,  G01N33/6842 ,  G01N33/6848

Abstract: An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins.

Abstract translation: 将整个补体或多个同位素标记的氨基酸引入测试对象的饮食中。 向受试者提供足够量的同位素标记的氨基酸，以确保受试者将大量同位素标记的氨基酸掺入新合成的蛋白质中。 在不同的时间点从受试者中取出组织样品，提取和分离蛋白质，以便不同组织的不同蛋白质可以单独分析，并且可以测定其同位素标记的数量和模式。 在优选的实施方案中，该方法可以与肽的蛋白水解消化和质谱分析相结合，以便测量与数千种不同蛋白质相关的体内蛋白质周转率。

公开(公告)号：US08674698B2

公开(公告)日：2014-03-18

申请号：US13170972

申请日：2011-06-28

Applicant: John C. Price

Inventor： John C. Price

IPC: G01V3/00

CPC classification number: G01R33/3664

Abstract: A single-pole, double-throw, transmit/receive switch for switching a NMR coil between a transmit mode and a receive mode comprises two diode bridge switches on opposites sides of a node the is common with the two diode bridges and the NMR coil. One of the two diode bridges is positioned between the coil and a preamplifier for signals received from the coil, and the other diode bridge is positioned between the coil and a RF signal transmitter for connecting and disconnecting transmitted signals to and from the coil. The NMR coil is connected to the switches through a resonant impedance matching network.

Abstract translation: 用于在发射模式和接收模式之间切换NMR线圈的单极双掷发射/接收开关包括在两个二极管桥和NMR线圈共同的节点的相对侧上的两个二极管桥接开关。 两个二极管桥之一位于线圈和用于从线圈接收的信号的前置放大器之间，另一个二极管桥位于线圈和RF信号发射器之间，用于连接和断开与线圈的传输信号。 NMR线圈通过谐振阻抗匹配网络连接到开关。

公开(公告)号：US5472935A

公开(公告)日：1995-12-05

申请号：US983632

申请日：1992-12-01

Applicant: Robert M. Yandrofski ,  John C. Price ,  Frank Barnes ,  Allen M. Hermann ,  James F. Scott

Inventor： Robert M. Yandrofski ,  John C. Price ,  Frank Barnes ,  Allen M. Hermann ,  James F. Scott

IPC: H01G7/06 ,  H01P1/18 ,  H01P7/08 ,  H01Q1/36 ,  H01Q3/44 ,  H01B12/02 ,  H01P9/00 ,  H03H11/16

CPC classification number: H01P7/082 ,  H01G7/06 ,  H01P1/181 ,  H01P7/084 ,  H01P7/088 ,  H01Q1/364 ,  H01Q3/44 ,  Y10S505/70 ,  Y10S505/701 ,  Y10S505/866

Abstract: The disclosure relates to ferroelectric and superconducting thin films used in combination to produce low-loss passive microwave and millimeter wave devices which are frequency tuneable. Various metal oxide superconducting and ferroelectric thin films can be deposited in numerous multilayer geometries via a variety of deposition techniques to produce devices which can manipulate microwave and millimeter wave signals through the application of voltage bias signals across the ferroelectric films. Numerous superconducting microwave and millimeter wave devices, including delay lines, phase shifters, resonators, oscillators, filters, electrically-small antennas, half-loop antennas, directional couplers, patch antennas, and various radiative gratings, are made frequency-tuneable by utilizing voltage-tuneable capacitor structures fabricated from voltage-biased ferroelectric thin films. Tuneable antenna arrays are also disclosed incorporating the combined superconducting and ferroelectric thin film structures and geometries which allow for electrical steering of beam patterns by the application of one or more voltage bias signals to ferroelectric thin film gratings.

Abstract translation: 本公开涉及组合使用的铁电和超导薄膜以产生可变频的低损耗无源微波和毫米波器件。 各种金属氧化物超导和铁电薄膜可以通过各种沉积技术以许多多层几何形状沉积，以产生能够通过在铁电体膜上施加电压偏置信号来操纵微波和毫米波信号的装置。 许多超导微波和毫米波器件，包括延迟线，移相器，谐振器，振荡器，滤波器，电子小天线，半环形天线，定向耦合器，贴片天线和各种辐射光栅，可以通过利用电压进行频率调谐 由电压偏置铁电薄膜制造的可调谐电容器结构。 还公开了可调谐天线阵列，其结合了组合的超导和铁电薄膜结构和几何形状，其允许通过将一个或多个电压偏置信号施加到铁电薄膜光栅来对波束图案进行电子操纵。

公开(公告)号：US09658213B2

公开(公告)日：2017-05-23

申请号：US13704498

申请日：2011-06-17

Applicant: Stanley B. Prusiner ,  John C. Price ,  Sina Ghaemmaghami ,  Al Burlingame ,  Shenheng Guan

Inventor： Stanley B. Prusiner ,  John C. Price ,  Sina Ghaemmaghami ,  Al Burlingame ,  Shenheng Guan

IPC: G01N33/50 ,  G01N33/68 ,  G01N27/26

CPC classification number: G01N33/5088 ,  G01N33/6842 ,  G01N33/6848

Abstract: An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins.

公开(公告)号：US20110316536A1

公开(公告)日：2011-12-29

申请号：US13171230

申请日：2011-06-28

Applicant: John C. Price ,  Charles Miller ,  Erick Winston

Inventor： John C. Price ,  Charles Miller ,  Erick Winston

IPC: G01R33/44

CPC classification number: G01R33/307 ,  G01R33/302 ,  G01R33/445

Abstract: A method is provided for acquiring multiple NMR response signal data in rapid succession for averaging NMR spectral data from a sample. The fluid sample is placed in a capillary that extends through the magnetic field of the NMR spectrometer, including through the center of the magnetic field to place a segment of the sample in the magnetic center. After the sample fluid, initially magnetized by the magnetic field, is activated to emit an NMR pulse signal, the fluid in the capillary is advanced rapidly to put another pre-magnetized segment of the sample fluid in the fluid center, acquiring an NRM pulse signal, and continuing the cycle until a desired number of NMR response data signals from the sample have been acquired. Those response data from multiple acquisitions are then averaged.

Abstract translation: 提供一种快速连续获取多个NMR响应信号数据的方法，用于对来自样品的NMR光谱数据进行平均。 将流体样品置于穿过NMR光谱仪的磁场的毛细管中，包括通过磁场的中心，将样品的一段放置在磁性中心。 在初始被磁场磁化的样品流体被激活以发射NMR脉冲信号之后，毛细管中的流体被快速前进，以将样品流体的另一个预先磁化的区段放在流体中心，获得NRM脉冲信号 并继续循环，直到获得了来自样品的所需数量的NMR响应数据信号。 然后对来自多次获取的响应数据进行平均。

公开(公告)号：US06628016B2

公开(公告)日：2003-09-30

申请号：US09812647

申请日：2001-03-20

Applicant: Josef Michl ,  John C. Price ,  Thomas F. Magnera

Inventor： Josef Michl ,  John C. Price ,  Thomas F. Magnera

IPC: C01B3308

CPC classification number: B82Y10/00 ,  B82Y30/00 ,  C07F7/12 ,  C07F17/00 ,  C07F17/02 ,  G11C11/22 ,  G11C13/02 ,  Y10S977/725 ,  Y10S977/731 ,  Y10T428/31663

Abstract: Molecular dipolar rotors comprising a base, an axle connected to said base and oriented substantially perpendicular to said base, and a rotor portion having an electric dipole moment are provided. The molecular dipolar rotors may be attached to a surface. Arrays of molecular dipolar rotors attached to surfaces are provided. Molecular dipolar rotors are useful in preparation of small devices.

Abstract translation: 分子偶极转子包括基座，连接到所述基座并且基本上垂直于所述基座定向的轴和具有电偶极矩的转子部分。 分子偶极转子可以附着在表面上。 提供了附着于表面的分子偶极子转子阵列。 分子双极转子可用于制备小型器件。

公开(公告)号：US08754646B2

公开(公告)日：2014-06-17

申请号：US13171230

申请日：2011-06-28

Applicant: John C. Price ,  Charles Miller ,  Erick Winston

Inventor： John C. Price ,  Charles Miller ,  Erick Winston

IPC: G01V3/00

CPC classification number: G01R33/307 ,  G01R33/302 ,  G01R33/445

Abstract: A method is provided for acquiring multiple NMR response signal data in rapid succession for averaging NMR spectral data from a sample. The fluid sample is placed in a capillary that extends through the magnetic field of the NMR spectrometer, including through the center of the magnetic field to place a segment of the sample in the magnetic center. After the sample fluid, initially magnetized by the magnetic field, is activated to emit an NMR pulse signal, the fluid in the capillary is advanced rapidly to put another pre-magnetized segment of the sample fluid in the fluid center, acquiring an NRM pulse signal, and continuing the cycle until a desired number of NMR response data signals from the sample have been acquired. Those response data from multiple acquisitions are then averaged.

Abstract translation: 提供一种快速连续获取多个NMR响应信号数据的方法，用于对来自样品的NMR光谱数据进行平均。 将流体样品置于穿过NMR光谱仪的磁场的毛细管中，包括通过磁场的中心，将样品的一段放置在磁性中心。 在初始被磁场磁化的样品流体被激活以发射NMR脉冲信号之后，毛细管中的流体被快速前进，以将样品流体的另一个预先磁化的区段放在流体中心，获得NRM脉冲信号 并继续循环，直到获得了来自样品的所需数量的NMR响应数据信号。 然后对来自多次获取的响应数据进行平均。

公开(公告)号：US08349080B1

公开(公告)日：2013-01-08

申请号：US12313766

申请日：2008-11-24

Applicant: John C. Price

Inventor： John C. Price

IPC: C30B35/00 ,  C30B13/28 ,  C30B15/30 ,  C30B13/00 ,  C30B21/04 ,  C30B28/08

CPC classification number: G02B21/26 ,  G02B21/22 ,  G02B21/32 ,  Y10T117/10 ,  Y10T117/1008 ,  Y10T117/108

Abstract: A micro-manipulator machine for harvesting and cryofreezing crystals for cryogenic storage and subsequent analysis includes a micropositioner mechanism for converting motions manually input to a position control knob to fractionally-scaled motions of a follower mechanism which includes a tool head support arm and tool head that releasably holds a filamentary polymer cryoloop for immersion into a liquid crystal growth media and extraction of a liquid drop containing a selected crystal from the media. A first automatic actuator mechanism orbits the tool head support arm, tool head, cryoloop, liquid drop and harvested crystal from a harvesting location to a retrieval location when the micropositioner input control arm has been moved manually away from the crystal harvesting location by the operator after extracting a crystal drop, and a second automatic actuator mechanism pivots the toll head into a flowing stream of a cryogenic gas to freeze the liquid drop and crystal.

Abstract translation: 一种用于收集和冻结晶体以用于低温储存和随后分析的微操纵机器包括微定位器机构，用于将手动输入的运动转换到位置控制旋钮到从动机构的分数运动，其包括工具头支撑臂和工具头， 可释放地保持用于浸入液晶生长介质的丝状聚合物冷冻液并从介质中萃取含有选定晶体的液滴。 当微型定位器输入控制臂在操作者手动移离晶体收集位置之后，第一自动致动器机构将工具头支撑臂，工具头，冷冻液，液滴和收集的晶体从收获位置旋转到检索位置。 提取出水滴，第二自动致动器机构将收费头枢转成低温气体的流动流，以冻结液滴和晶体。

公开(公告)号：US20110001474A1

公开(公告)日：2011-01-06

申请号：US12635697

申请日：2009-12-10

Applicant: Charles E. Miller ,  John C. Price

Inventor： Charles E. Miller ,  John C. Price

IPC: G01R33/44

CPC classification number: G01N24/08 ,  G01F1/56 ,  G01N24/081 ,  G01R33/383 ,  G01R33/3873 ,  G01R33/44 ,  G01R33/56308

Abstract: Methods and apparatus for obtaining NMR signals from a flowing fluid include permanent magnet assemblies for producing magnetic fields for NMR applications and instrumentations, including, but not limited to, flow metering.

Abstract translation: 用于从流动流体获得NMR信号的方法和装置包括用于产生用于NMR应用和仪器的磁场的永磁体组件，包括但不限于流量计量。

公开(公告)号：US20120001636A1

公开(公告)日：2012-01-05

申请号：US13171222

申请日：2011-06-28

Applicant: John C. Price ,  Michael Steinbach ,  Chris Jonkman

Inventor： John C. Price ,  Michael Steinbach ,  Chris Jonkman

IPC: G01R33/30

CPC classification number: G01R33/302 ,  G01R33/3875

Abstract: A shim coil assembly for positioning shim coils in a narrow gap between magnetic poles of miniature NMR devices around the magnetic center includes electric traces on multiple levels of printed circuit board layers in various, overlapping configurations. Mirror image shim coils can be connected in series in a symmetric sense or connected in series in a antisymmetric sense.

Abstract translation: 用于将薄片线圈定位在围绕磁性中心的微型NMR装置的磁极之间的窄间隙中的垫片组件包括在各种重叠构型的多层印刷电路板层上的电迹线。 镜像片状线圈可以对称连续串联连接或以反对称的方式串联连接。