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公开(公告)号:US20120030834A1
公开(公告)日:2012-02-02
申请号:US12935266
申请日:2009-04-10
申请人: John D. Bradley , Brandi Chiapelli , Bingli Gao , James P. McCarter , Michelle L. Verbsky , Deryck Jeremy Williams
发明人: John D. Bradley , Brandi Chiapelli , Bingli Gao , James P. McCarter , Michelle L. Verbsky , Deryck Jeremy Williams
IPC分类号: C12N15/82 , C12N15/63 , C12N1/00 , C12N5/10 , A01H5/00 , A01H5/10 , A01N63/02 , C12Q1/68 , C07H21/02 , C12N15/113 , C12N1/19 , C12N1/15 , A01P5/00 , C07K14/415 , C11B1/00 , C08B30/00 , A23L1/212 , A23D9/00 , C07H21/04
CPC分类号: C12N15/8285 , A01H4/008 , A01N57/16 , A01N63/02 , A01N65/00 , C07K14/4354 , C12N15/113 , C12N15/8218 , C12N15/8261 , C12N15/8273 , C12N2310/14 , Y02A40/164
摘要: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.
摘要翻译: 本发明公开了由根结线虫(根结线虫)的线虫引起的植物病害遗传控制的基因靶标,构建体和方法。 本发明涉及通过鉴定靶编码序列和使用重组DNA技术来实现植物保护作用,用于转录后抑制或抑制植物寄生线虫细胞中靶编码序列的表达。 所公开的基因靶标显示在来自不同的根结线虫种类的直向同源物之间的核苷酸水平上的显着的保守性,通过RNA干扰促进了属于全局的靶向。
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公开(公告)号:US08907162B2
公开(公告)日:2014-12-09
申请号:US13519929
申请日:2010-12-29
申请人: Brandi Chiapelli , Matt W. Dimmic , Deryck Jeremy Williams , Jennifer Sheppard , John D. Bradley , Carl Diehl , Michelle L. Gasper , Bingli Gao , James P. McCarter
发明人: Brandi Chiapelli , Matt W. Dimmic , Deryck Jeremy Williams , Jennifer Sheppard , John D. Bradley , Carl Diehl , Michelle L. Gasper , Bingli Gao , James P. McCarter
CPC分类号: C12N15/8285 , C12N15/113 , C12N15/8218 , C12N2310/14 , Y02A40/164
摘要: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Pratylenchus (root lesion nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets are conserved and expected to be essential and sensitive to RNAi perturbation in different Pratylenchus species, facilitating genus-wide targeting by RNA interference.
摘要翻译: 本发明公开了由Pratylenchus属(线虫线虫)线虫引起的植物病害遗传控制的基因靶标,构建体和方法。 本发明涉及通过鉴定靶编码序列和使用重组DNA技术来实现植物保护作用,用于转录后抑制或抑制植物寄生线虫细胞中靶编码序列的表达。 所公开的基因靶点是保守的,并且预期对于不同Pratylenchus物种中的RNAi扰动是必需的并且是敏感的,通过RNA干扰促进基因范围的靶向。
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公开(公告)号:US20130036519A1
公开(公告)日:2013-02-07
申请号:US13519929
申请日:2010-12-29
申请人: Brandi Chiapelli , Matt W. Dimmic , Deryck Jeremy Williams , Jennifer Sheppard , John D. Bradley , Carl Diehl , Michelle L. Gasper , Bingli Gao , James P. McCarter
发明人: Brandi Chiapelli , Matt W. Dimmic , Deryck Jeremy Williams , Jennifer Sheppard , John D. Bradley , Carl Diehl , Michelle L. Gasper , Bingli Gao , James P. McCarter
IPC分类号: A01H5/00 , C12N15/82 , C12N5/10 , C40B30/06 , A01H5/10 , A23L1/212 , A01P5/00 , C11B1/00 , C07K14/415 , A23L1/10 , A23D9/00 , A23K1/00 , C12N15/113 , A01N57/16
CPC分类号: C12N15/8285 , C12N15/113 , C12N15/8218 , C12N2310/14 , Y02A40/164
摘要: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Pratylenchus (root lesion nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets are conserved and expected to be essential and sensitive to RNAi perturbation in different Pratylenchus species, facilitating genus-wide targeting by RNA interference.
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公开(公告)号:US07339044B2
公开(公告)日:2008-03-04
申请号:US11191588
申请日:2005-07-28
申请人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
发明人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
CPC分类号: C07K14/705
摘要: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
摘要翻译: 描述了编码含有PAN和ZP结构域的受体多肽的线虫的核酸分子。 还提供PANZP多肽序列,载体,宿主细胞以及用于产生PANZP核苷酸和多肽的重组方法。 还描述了用于鉴定抑制剂和/或激活剂的筛选方法以及用于抗体生产的方法。
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公开(公告)号:US07265204B2
公开(公告)日:2007-09-04
申请号:US11191375
申请日:2005-07-28
申请人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
发明人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
CPC分类号: C07K14/705
摘要: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
摘要翻译: 描述了编码含有PAN和ZP结构域的受体多肽的线虫的核酸分子。 还提供PANZP多肽序列,载体,宿主细胞以及用于产生PANZP核苷酸和多肽的重组方法。 还描述了用于鉴定抑制剂和/或激活剂的筛选方法以及用于抗体生产的方法。
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公开(公告)号:US20080227955A1
公开(公告)日:2008-09-18
申请号:US12042297
申请日:2008-03-04
申请人: Michelle Coutu Hresko , Merry B. Mclaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
发明人: Michelle Coutu Hresko , Merry B. Mclaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
IPC分类号: C07K14/435
CPC分类号: C07K14/705
摘要: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
摘要翻译: 描述了编码含有PAN和ZP结构域的受体多肽的线虫的核酸分子。 还提供PANZP多肽序列,载体,宿主细胞以及用于产生PANZP核苷酸和多肽的重组方法。 还描述了用于鉴定抑制剂和/或激活剂的筛选方法以及用于抗体生产的方法。
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公开(公告)号:US07250500B2
公开(公告)日:2007-07-31
申请号:US11191374
申请日:2005-07-28
申请人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
发明人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
CPC分类号: C07K14/705
摘要: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
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公开(公告)号:US06936693B2
公开(公告)日:2005-08-30
申请号:US10771708
申请日:2004-02-04
申请人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
发明人: Michelle Coutu Hresko , Merry B. McLaird , Deryck J. Williams , Anita M. Frevert , Brandi Chiapelli , Catherine Baublite , Andrew P. Kloek , Jennifer A. Davila-Aponte , John D. Bradley , Siqun Xu
IPC分类号: A61K38/17 , C07H21/04 , C07K1/00 , C07K14/00 , C07K14/435 , C07K14/705 , C07K17/00 , C12N20060101 , C12N15/00 , C12P21/06 , C12Q1/68
CPC分类号: C07K14/705
摘要: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
摘要翻译: 描述了编码含有PAN和ZP结构域的受体多肽的线虫的核酸分子。 还提供PANZP多肽序列,载体,宿主细胞以及用于产生PANZP核苷酸和多肽的重组方法。 还描述了用于鉴定抑制剂和/或激活剂的筛选方法以及用于抗体生产的方法。
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公开(公告)号:US20050282222A1
公开(公告)日:2005-12-22
申请号:US11191588
申请日:2005-07-28
申请人: Michelle Hresko , Merry McLaird , Deryck Williams , Anita Frevert , Brandi Chiapelli , Catherine Baublite , Andrew Kloek , Jennifer Davila-Aponte , John Bradley , Siqun Xu
发明人: Michelle Hresko , Merry McLaird , Deryck Williams , Anita Frevert , Brandi Chiapelli , Catherine Baublite , Andrew Kloek , Jennifer Davila-Aponte , John Bradley , Siqun Xu
IPC分类号: A61K38/17 , C07H21/04 , C07K1/00 , C07K14/00 , C07K14/435 , C07K14/705 , C07K17/00 , C12N20060101 , C12N15/00 , C12P21/06 , C12Q1/68
CPC分类号: C07K14/705
摘要: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
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公开(公告)号:US20050260674A1
公开(公告)日:2005-11-24
申请号:US11191375
申请日:2005-07-28
申请人: Michelle Hresko , Merry McLaird , Deryck Williams , Anita Frevert , Brandi Chiapelli , Catherine Baublite , Andrew Kloek , Jennifer Davila-Aponte , John Bradley , Siqun Xu
发明人: Michelle Hresko , Merry McLaird , Deryck Williams , Anita Frevert , Brandi Chiapelli , Catherine Baublite , Andrew Kloek , Jennifer Davila-Aponte , John Bradley , Siqun Xu
IPC分类号: A61K38/17 , C07H21/04 , C07K1/00 , C07K14/00 , C07K14/435 , C07K14/705 , C07K17/00 , C12N20060101 , C12N15/00 , C12P21/06 , C12Q1/68
CPC分类号: C07K14/705
摘要: Nucleic acid molecules from nematodes encoding PAN and ZP domain containing receptor polypeptides are described. PANZP polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of PANZP nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.
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