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公开(公告)号：US08372798B2
公开(公告)日：2013-02-12
申请号：US10271832
申请日：2002-10-16
IPC分类号： A61K49/14
CPC分类号： A61K49/14 , A61K9/0019 , A61K9/146 , A61K39/39591 , A61K47/10 , A61K47/26
摘要： Provided is a pharmaceutical composition involving a concentrated mixture of a precipitated protein and a liquid medium and a method for manufacturing the concentrated mixture. The concentrated mixture is manufacturable by precipitation of a protein with a biocompatible polymer precipitating agent, followed by removal of sufficient liquid to concentrate the resulting mixture to the desired degree. The precipitated protein can be stored for a significant time in the concentrated mixture, such as intermediate between processing stages during manufacture operations.
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公开(公告)号：US20110243996A1
公开(公告)日：2011-10-06
申请号：US13065965
申请日：2011-04-01
CPC分类号： A61K9/0043 , A61K9/1617 , A61K9/1623 , A61K9/1658 , A61K9/1694
摘要： This invention provides methods and compositions to preserve bioactive materials, such as peptides, nucleic acids, viruses, bacteria, cells, or liposomes, in freeze dried particles suitable for intranasal administration. Methods provide spray freeze drying of formulations to form stable freeze dried particles for intranasal administration.
摘要翻译： 本发明提供在适于鼻内给药的冷冻干燥颗粒中保存生物活性物质如肽，核酸，病毒，细菌，细胞或脂质体的方法和组合物。 方法提供制剂的喷雾冷冻干燥以形成用于鼻内给药的稳定的冷冻干燥颗粒。
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公开(公告)号：US20100330162A1
公开(公告)日：2010-12-30
申请号：US12807343
申请日：2010-09-01
IPC分类号： A61K9/127 , A61K39/145 , A61K9/14 , A61P31/12 , A61P31/04
CPC分类号： A61K9/0075 , A01N1/02 , A01N1/0252 , A61K9/1694 , A61K9/19
摘要： This invention provides methods and compositions to preserve bioactive materials, such as viruses, bacteria, cells, or liposomes, in freeze dried particles suitable for pulmonary administration. Methods provide spray freeze drying of formulations to form stable freeze dried particles.
摘要翻译： 本发明提供了在适于肺部给药的冷冻干燥颗粒中保存生物活性物质如病毒，细菌，细胞或脂质体的方法和组合物。 方法提供喷雾冷冻干燥制剂以形成稳定的冻干颗粒。
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公开(公告)号：US06489450B2
公开(公告)日：2002-12-03
申请号：US09350327
申请日：1999-07-09
IPC分类号： A23J100
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译： 本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。 重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。 通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。 该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。
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公开(公告)号：US5160313A
公开(公告)日：1992-11-03
申请号：US699873
申请日：1991-05-14
CPC分类号： A61L27/3687 , A01N1/0221 , A01N1/0278 , Y10S128/27 , Y10S623/901
摘要： A method is disclosed for preparing a transplantable tissue which has been cryopreserved with an intracellular cryoprotectant and then thawed. After thawing, the tissue is treated with a diluent (or eluent) solution to reduce the level of cryoprotectant in the cells to a substantially non-toxic level. This is conducted using a single dilution step. Cryoprotectants which are removable in this manner include dimethylsulfoxide, glycerol, propanediol and other compounds which penetrate the cells.The dilution step does not require end point titration and can be conducted over a time period greater than about 5 minutes.
摘要翻译： 公开了一种用于制备已经用细胞内冷冻保护剂冷冻保存然后解冻的可移植组织的方法。 解冻后，用稀释剂（或洗脱液）溶液处理组织以将细胞中的冷冻保护剂的水平降低到基本上无毒的水平。 这是使用单次稀释步骤进行的。 以这种方式可去除的冷冻保护剂包括二甲基亚砜，甘油，丙二醇和其它穿透细胞的化合物。 稀释步骤不需要终点滴定，并且可以在大于约5分钟的时间内进行。
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公开(公告)号：US08710197B2
公开(公告)日：2014-04-29
申请号：US12940587
申请日：2010-11-05
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译： 本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。 重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。 通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。 该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。
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公开(公告)号：US20110046357A1
公开(公告)日：2011-02-24
申请号：US12940587
申请日：2010-11-05
IPC分类号： C07K1/14
CPC分类号： C07K14/61 , C07K1/1133 , C07K1/1136 , C12N9/2462
摘要： The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
摘要翻译： 本公开提供了在溶液中重折叠变性蛋白质的有效方法，从而以高产率回收溶液中适当折叠的生物活性蛋白质。 重折叠在约0.25kbar至约3.5kbar之间，有利地为约1.5kbar至约3kbar的压力下进行。 通常，离液剂以对大气压下变性蛋白质无效的浓度存在，并且任选地，氧化还原试剂可以并入再折叠溶液中，使得可以在需要的地方形成天然的分子内二硫键。 该方法适用于基本上所有蛋白质，特别是在溶解和/或变性不溶性蛋白质聚集体，包涵体或异常低聚（可溶））聚集体之后。
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8.发明申请Method of Preparing an Immunologically-Active Adjuvant-Bound Dried Vaccine Composition 有权制备免疫活性佐剂干燥疫苗组合物的方法公开(公告)号：US20100158951A1
公开(公告)日：2010-06-24
申请号：US12532225
申请日：2008-03-18
CPC分类号： A61K47/48015 , A61K9/19 , A61K39/08 , A61K2039/55505 , A61K2039/55511 , A61K2039/60 , A61K2039/622 , Y02A50/469
摘要： The disclosure provides a method of preparing an immunologically-active adjuvant-bound freeze dried vaccine composition. A specific embodiment provides a stable vaccine composition comprising an aluminum-salt adjuvant, a recombinant Clostridium botulinum neurotoxin protein and a glass-forming agent. These vaccine compositions are useful in the treatment of humans and other animals at risk of infection from Clostridium botulinum neurotoxin.
摘要翻译： 本公开提供了制备免疫学活性佐剂结合的冷冻干燥疫苗组合物的方法。 具体实施方案提供了包含铝盐佐剂，重组肉毒杆菌神经毒素蛋白质和玻璃形成剂的稳定的疫苗组合物。 这些疫苗组合物可用于治疗肉毒杆菌神经毒素感染风险的人和其他动物。
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9.发明申请公开(公告)号：US20080248522A1
公开(公告)日：2008-10-09
申请号：US12098877
申请日：2008-04-07
摘要： Described herein are methods for the solubilization and proteolytic cleavage of fusion protein aggregates, including autocatalytic fusion proteins, at pressures greater than atmospheric pressure to yield soluble target polypeptides.
摘要翻译： 本文描述了在大于大气压的压力下溶解和蛋白水解切割融合蛋白聚集体（包括自催化融合蛋白）以产生可溶性靶多肽的方法。
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10.发明授权
公开(公告)号：US5171660A
公开(公告)日：1992-12-15
申请号：US344013
申请日：1989-04-26
IPC分类号： A01N1/02
CPC分类号： A01N1/0284
摘要： A method of revitalizing cells or tissues that are to be cryopresserved for storage at ultracold temperatures, e.g. -196.degree. C. is disclosed which comprises preincubation of the cells or tissue from about 5 minutes to about 24 hours. The preincubation may be conducted at a temperature ranging from about 27.degree. C. to 42.degree. C., after which the tissue or cells are cryopreserved.
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