公开(公告)号：US20070202535A1

公开(公告)日：2007-08-30

申请号：US10596692

申请日：2004-12-24

申请人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama

发明人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama

IPC分类号： G01N33/53 ,  C07K14/705

CPC分类号： G01N33/54393 ,  G01N33/543 ,  Y10S435/97

摘要： As a result of investigating the optimum conditions of methods for immobilizing proteins that interact with sugar chains onto a substrate, it was revealed that coating the surface of a slide glass with GTMS enables immobilization at a higher S/N ratio than conventionally possible. Moreover, by using a substrate to which a rubber with a number of holes was affixed to form a number of reaction vessels, and further by spotting lectins onto the substrate and washing with PBST, the weak interactions between sugar chains and lectins were successfully detected with improved sensitivity. In addition, by introducing an evanescent excitation-type scanner, it became possible to detect the interactions between lectins and sugar chains without washing away the probe solution.

摘要翻译： 通过研究将与糖链相互作用的蛋白质固定在基质上的最佳条件的结果，显示用GTMS涂覆载玻片的表面使得能够以比传统可能的更高的S / N比固定。 此外，通过使用固定有多个孔的橡胶的基板以形成多个反应容器，并且进一步通过将凝集素点样到基板上并用PBST洗涤，成功检测到糖链和凝集素之间的弱相互作用 提高灵敏度。 此外，通过引入ev逝激发型扫描仪，可以检测凝集素和糖链之间的相互作用而不洗掉探针溶液。

公开(公告)号：US08008094B2

公开(公告)日：2011-08-30

申请号：US10596692

申请日：2004-12-24

申请人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama

发明人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama

IPC分类号： G01N33/53

CPC分类号： G01N33/54393 ,  G01N33/543 ,  Y10S435/97

摘要： As a result of investigating the optimum conditions of methods for immobilizing proteins that interact with sugar chains onto a substrate, it was revealed that coating the surface of a slide glass with GTMS enables immobilization at a higher S/N ratio than conventionally possible. Moreover, by using a substrate to which a rubber with a number of holes was affixed to form a number of reaction vessels, and further by spotting lectins onto the substrate and washing with PBST, the weak interactions between sugar chains and lectins were successfully detected with improved sensitivity. In addition, by introducing an evanescent excitation-type scanner, it became possible to detect the interactions between lectins and sugar chains without washing away the probe solution.

摘要翻译： 通过研究将与糖链相互作用的蛋白质固定在基质上的最佳条件的结果，显示用GTMS涂覆载玻片的表面使得能够以比传统可能的更高的S / N比固定。 此外，通过使用固定有多个孔的橡胶的基板以形成多个反应容器，并且进一步通过将凝集素点样到基板上并用PBST洗涤，成功检测到糖链和凝集素之间的弱相互作用 提高灵敏度。 此外，通过引入ev逝激发型扫描仪，可以检测凝集素和糖链之间的相互作用而不洗掉探针溶液。

公开(公告)号：US20070167608A1

公开(公告)日：2007-07-19

申请号：US10596704

申请日：2004-06-30

申请人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama ,  Ken-ichi Kasai ,  Yoichiro Arata ,  Yoriko Takahashi

发明人： Jun Hirabayashi ,  Atsushi Kuno ,  Sachiko Nakamura ,  Noboru Uchiyama ,  Ken-ichi Kasai ,  Yoichiro Arata ,  Yoriko Takahashi

IPC分类号： C07K14/00

CPC分类号： G01N33/543 ,  G01N30/02 ,  G01N30/8679 ,  G01N33/54366 ,  G01N2030/8813 ,  B01D15/428

摘要： The present inventors discovered that lectins recognize extremely small differences in sugar chain structures, and that by using this ability to recognize sugar chain structures, numerous sugar chain structures can be distinguished using the strong or weak interaction patterns of about ten types of lectins. Therefore, by reference to and use of control interaction data, in which large amounts of data on the interactions between sugar chains and lectins have been collected, the structures of subject sugar chains can be identified, estimated, and such with high accuracy.

摘要翻译： 本发明人发现，凝集素识别糖链结构的极小差异，并且通过使用这种能力来识别糖链结构，许多糖链结构可以使用约10种凝集素的强或弱相互作用模式进行区分。 因此，通过参考和使用控制相互作用数据，其中收集了大量关于糖链和凝集素之间的相互作用的数据，可以高精度地鉴定，估计主题糖链的结构。

公开(公告)号：US20120053089A1

公开(公告)日：2012-03-01

申请号：US13213596

申请日：2011-08-19

申请人： Jun Hirabayashi ,  Atsushi Kuno ,  Noboru Uchiyama

发明人： Jun Hirabayashi ,  Atsushi Kuno ,  Noboru Uchiyama

IPC分类号： C40B40/10

CPC分类号： G01N33/54373 ,  G01N21/648 ,  G01N33/54393 ,  G01N33/548

摘要： An analyzer of the present invention for analyzing a glycan or a complex glycan includes a substrate, a fluorescent labeling excitation means and a fluorescent intensity measurement means.The substrate comprises a rectangle photoconductive base plate coated by a compound containing an active group to fix protein by an amino group thereof, one and more open-topped reaction vessel formed on a surface of the base plate, and plural spots of glycan binding proteins arranged in a matrix and immobilized on the surface of the base plate in the reaction vessel.The fluorescent labeling excitation means which excites fluorescent label by generating evanescent-waves on the surface of the substrate comprises a pair of optical fibers arranged toward the both side end faces of the right and left of the substrate to introduce a light thereinto.The fluorescent intensity measurement means for measuring the intensity of fluorescence generated by the fluorescent labeling excitation means on every spots of arranging the glycan binding protein.By using this analyzer, a glycan or a complex carbohydrate can be analyzed conveniently, quickly, sensitively and accurately.

摘要翻译： 用于分析聚糖或复合聚糖的本发明的分析装置包括基板，荧光标记激励装置和荧光强度测量装置。 基板包括由含有活性基团的化合物包被以氨基固定蛋白质的矩形光导基板，在基板的表面上形成的一个或多个开放顶部的反应容器，以及布置的多个聚糖结合蛋白点 在基质中并固定在反应容器中的基板的表面上。 通过在衬底的表面上产生ev逝波来激发荧光标记的荧光标记激发装置包括一对光纤，其布置成朝向衬底的左右两侧的端面以向其中引入光。 荧光强度测定装置，用于测量在布置聚糖结合蛋白的每个点上由荧光标记激发装置产生的荧光强度。 通过使用该分析仪，可以方便，快速，灵敏和准确地分析聚糖或复合碳水化合物。

公开(公告)号：US08597576B2

公开(公告)日：2013-12-03

申请号：US13213596

申请日：2011-08-19

申请人： Jun Hirabayashi ,  Atsushi Kuno ,  Noboru Uchiyama

发明人： Jun Hirabayashi ,  Atsushi Kuno ,  Noboru Uchiyama

IPC分类号： G01N21/00

CPC分类号： G01N33/54373 ,  G01N21/648 ,  G01N33/54393 ,  G01N33/548

摘要： An analyzer of the present invention for analyzing a glycan or a complex glycan includes a substrate, a fluorescent labeling excitation means and a fluorescent intensity measurement means. The substrate comprises a rectangle photoconductive base plate coated by a compound containing an active group to fix protein by an amino group thereof, one and more open-topped reaction vessel formed on a surface of the base plate, and plural spots of glycan binding proteins arranged in a matrix and immobilized on the surface of the base plate in the reaction vessel. The fluorescent labeling excitation means which excites fluorescent label by generating evanescent-waves on the surface of the substrate comprises a pair of optical fibers arranged toward the both side end faces of the right and left of the substrate to introduce a light thereinto.

摘要翻译： 用于分析聚糖或复合聚糖的本发明的分析装置包括基板，荧光标记激励装置和荧光强度测量装置。 基板包括由含有活性基团的化合物包被以氨基固定蛋白质的矩形光导基板，在基板的表面上形成的一个或多个开放顶部的反应容器，以及布置的多个聚糖结合蛋白点 在基质中并固定在反应容器中的基板的表面上。 通过在衬底的表面上产生ev逝波来激发荧光标记的荧光标记激发装置包括一对光纤，其布置成朝向衬底的左右两侧端面以向其中引入光。

公开(公告)号：US20090041630A1

公开(公告)日：2009-02-12

申请号：US11917921

申请日：2006-06-14

申请人： Jun Hirabayashi ,  Atsushi Kuno ,  Noboru Uchiyama

发明人： Jun Hirabayashi ,  Atsushi Kuno ,  Noboru Uchiyama

IPC分类号： G01N21/64

CPC分类号： G01N33/54373 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54393 ,  G01N33/548

摘要： An analyzer for a glycan or a complex carbohydrate which comprises a substrate made of a photoconductive material having multiple types of glycan binding proteins provided and immobilized thereon a means of conducting light into the side edge face of the substrate and generating an evanescent wave on the substrate surface to thereby excite fluorescent labels, and a fluorescent intensity-measuring means of measuring the intensity of the fluorescence induced by the above means for each of the positions of the glycan binding proteins provided as described above.By using this analyzer, a glycan or a complex carbohydrate can be conveniently, quickly, highly sensitively and highly accurately analyzed.

摘要翻译： 用于聚糖或复合碳水化合物的分析仪，其包含由具有多种类型的聚糖结合蛋白的光电导材料制成的基底，所述基质提供并固定在其上，将光导入基片的侧边缘并在基底上产生ev逝波 表面，从而激发荧光标记;以及荧光强度测定单元，其通过上述方法对上述提供的聚糖结合蛋白的各个位置测定由上述方法诱导的荧光强度。 通过使用该分析仪，可以方便，快速，高灵敏度和高度准确地分析聚糖或复合碳水化合物。

公开(公告)号：US20120190576A1

公开(公告)日：2012-07-26

申请号：US13384031

申请日：2010-07-12

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

IPC分类号： C40B30/04 ,  C07K14/00

CPC分类号： C07K9/00 ,  C07K14/47 ,  G01N33/57438 ,  G01N33/57469 ,  G01N2400/02 ,  G01N2800/085

摘要： The present invention is directed to developing a glycan markers capable of detecting a hepatic disease, and more specifically to developing a glycan marker indicating a hepatic disease-state. Furthermore, the present invention is also directed to developing a glycan marker capable of distinguishing hepatic disease-states with the progress of hepatocarcinoma. The present inventors identified, among the serum glycoproteins, glycopeptides and glycoproteins in which a glycan structure specifically changes due to a hepatic diseases including hepatocarcinoma and provide these as novel glycan markers (glycopeptide and glycoprotein) specific to hepatic disease-states.

摘要翻译： 本发明涉及开发能够检测肝脏疾病的聚糖标记物，更具体地说，涉及发展表达肝病状态的聚糖标记物。 此外，本发明还涉及开发能够区分肝脏疾病状态与肝癌进展的聚糖标记。 本发明人鉴定了血清糖蛋白中由于包括肝癌在内的肝脏疾病而特异性地改变聚糖结构的糖肽和糖蛋白，并将其提供为肝脏疾病状态特异性的新型聚糖标记（糖肽和糖蛋白）。

公开(公告)号：US09796761B2

公开(公告)日：2017-10-24

申请号：US13384031

申请日：2010-07-12

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

IPC分类号： C40B30/04 ,  C07K14/00 ,  C07K9/00 ,  C07K14/47 ,  G01N33/574

CPC分类号： C07K9/00 ,  C07K14/47 ,  G01N33/57438 ,  G01N33/57469 ,  G01N2400/02 ,  G01N2800/085

摘要： The present invention is directed to developing a glycan markers capable of detecting a hepatic disease, and more specifically to developing a glycan marker indicating a hepatic disease-state. Furthermore, the present invention is also directed to developing a glycan marker capable of distinguishing hepatic disease-states with the progress of hepatocarcinoma. The present inventors identified, among the serum glycoproteins, glycopeptides and glycoproteins in which a glycan structure specifically changes due to a hepatic diseases including hepatocarcinoma and provide these as novel glycan markers (glycopeptide and glycoprotein) specific to hepatic disease-states.

公开(公告)号：US20140323324A1

公开(公告)日：2014-10-30

申请号：US13994435

申请日：2011-12-16

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Atsushi Kuno ,  Hideki Matsuzaki ,  Yuzuru Ikehara ,  Hiromi Ito ,  Yasuhiro Hashimoto ,  Keiro Shirotani ,  Satoshi Futakawa ,  Hajime Arai ,  Masakazu Miyajima ,  Kazuo Fujihara

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Atsushi Kuno ,  Hideki Matsuzaki ,  Yuzuru Ikehara ,  Hiromi Ito ,  Yasuhiro Hashimoto ,  Keiro Shirotani ,  Satoshi Futakawa ,  Hajime Arai ,  Masakazu Miyajima ,  Kazuo Fujihara

IPC分类号： G01N33/68

CPC分类号： G01N33/6896 ,  G01N33/6842 ,  G01N2333/47 ,  G01N2333/4728 ,  G01N2400/40 ,  G01N2800/285 ,  H01J49/0036

摘要： The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.

摘要翻译： 本发明的目的是开发：从体液或中枢神经系统细胞中选择性分离衍生自中枢神经系统的糖蛋白的方法; 以及利用上述方法搜索中枢神经系统疾病指标的方法。 可以通过两步分离方法选择性地富集来自中枢神经系统的蛋白质，其在体液或中枢神经系统细胞中发生微量的蛋白质，其包括在非还原末端除去具有唾液酸的糖蛋白 从体液或中枢神经系统细胞中分离，然后在其非还原末端分离具有N-乙酰葡糖胺的糖蛋白。

公开(公告)号：US08557602B2

公开(公告)日：2013-10-15

申请号：US13203959

申请日：2010-02-23

申请人： Atsushi Kuno ,  Jun Hirabayashi ,  Atsushi Matsuda ,  Hisashi Narimatsu ,  Yuzuru Ikehara ,  Junichi Shoda ,  Toru Kawamoto

发明人： Atsushi Kuno ,  Jun Hirabayashi ,  Atsushi Matsuda ,  Hisashi Narimatsu ,  Yuzuru Ikehara ,  Junichi Shoda ,  Toru Kawamoto

IPC分类号： C40B30/04 ,  G01N33/53 ,  G01N33/574

CPC分类号： G01N21/648 ,  G01N33/57407 ,  G01N33/57438 ,  G01N33/57446

摘要： Disclosed are a method for early, sensitively and reliably detecting and distinguishing intrahepatic cholangiocarcinoma in a malignant tumor occurring primarily in the liver in a simple way, and a kit thereof. In the method, a glycan biomarker consisting of a lectin WFA (Wisteria floribunda Agglutinin)-binding glycoprotein derived from intrahepatic cholangiocarcinoma is used as a cancer marker to detect intrahepatic cholangiocarcinoma by detecting the cancer marker in a test specimen. The method for detecting intrahepatic cholangiocarcinoma can clearly differentiate intrahepatic cholangiocarcinoma from hepatocellular carcinoma and enables early detection and determination with a performance clinically acceptable in terms of applicability, sensitivity and precision.

摘要翻译： 公开了一种以简单的方式早期，敏感和可靠地检测和区分主要在肝脏中发生的恶性肿瘤中的肝内胆管癌的方法及其试剂盒。 在该方法中，将由凝血素WFA（Wisteria floribunda凝集素）结合的糖蛋白组成的聚糖生物标志物作为肝内胆管癌的检测用于检测肝内胆管癌的癌症标志物。 检测肝内胆管癌的方法可以明确区分肝内胆管癌与肝细胞癌，能够在适用性，敏感性和准确性方面具有临床可接受的临床诊断和测定能力。