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    NUCLEIC ACID DETECTION DEVICE
    1.
    发明申请
    NUCLEIC ACID DETECTION DEVICE 审中-公开
    核酸检测装置

    公开(公告)号：US20080233634A1

    公开(公告)日：2008-09-25

    申请号：US12048690

    申请日：2008-03-14

    申请人： Jun OKADA ,  Sadato Hongo ,  Keiko Ito ,  Kenji Ooki ,  Nobuhiro Gemma

    发明人： Jun OKADA ,  Sadato Hongo ,  Keiko Ito ,  Kenji Ooki ,  Nobuhiro Gemma

    IPC分类号： C12M1/00

    CPC分类号： G01N35/085

    摘要： A nucleic acid detection device is provided with a closed channel formed of a first channel portion, through which a washing reagent storage section for storing a washing reagent for washing a detection section for nucleic acid detection communicates with the detection section, and a second channel portion through which a pretreatment section for nucleic acid treatment communicates with the detection section. The closed channel is connected with a gas inlet/outlet path for communication with the outside. The gas inlet/outlet path is blocked by a sealing mechanism before nucleic acid detection. In storing a pretreatment reagent and the washing reagent frozen, the gas inlet/outlet path is kept open and connected to the channel. Thus, there is provided a nucleic acid detection device having a structure for preventing leakage of nucleic acid samples to the outside and which can be stored for a long period of time with the reagents therein.

    摘要翻译： 核酸检测装置设置有由第一通道部分形成的封闭通道，用于存储用于清洗用于核酸检测的检测部分的洗涤剂的洗涤剂存储部分与检测部分通信，第二通道部分 用于核酸处理的预处理部分与检测部分通信。 封闭通道与用于与外部连通的气体入口/出口路径连接。 在核酸检测之前，气体入口/出口路径被密封机构堵塞。 在储存预处理试剂和洗涤剂冻结时，气体入口/出口路径保持打开并连接到通道。 因此，提供了一种核酸检测装置，其具有防止核酸样品向外部泄漏的结构，并且可以用其中的试剂长时间储存​​。

    METHOD OF DETECTING NUCLEIC ACID AMPLIFICATION REACTION
    3.
    发明申请
    METHOD OF DETECTING NUCLEIC ACID AMPLIFICATION REACTION 审中-公开
    检测核酸放大反应的方法

    公开(公告)号：US20100099094A1

    公开(公告)日：2010-04-22

    申请号：US12498721

    申请日：2009-07-07

    申请人： Jun OKADA ,  Nobuhiro Gemma

    发明人： Jun OKADA ,  Nobuhiro Gemma

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/682 ,  C12Q1/6825 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2563/113

    摘要： The present invention provides a method of detecting a nucleic acid amplification reaction, including the steps of adding a sample nucleic acid to a nucleic acid amplification buffer containing a reducing agent molecule, a redox molecule and a magnesium ion, to conduct an amplification reaction, measuring a reduction current produced by a reduction reaction of the reducing agent molecule with the redox molecule, under the conditions that when the amplification reaction of the sample nucleic acid has proceeded in the buffer, pyrophosphoric acid formed with the progress of amplification of the sample nucleic acid forms magnesium pyrophosphate with the magnesium ion, thereby decreasing a magnesium ion concentration of the buffer, and determining, from the magnitude of the reduction current measured above, whether the sample nucleic acid has been amplified or not.

    摘要翻译： 本发明提供一种检测核酸扩增反应的方法，包括以下步骤：将样品核酸加入到含有还原剂分子，氧化还原分子和镁离子的核酸扩增缓冲液中进行扩增反应，测定 在还原剂分子与氧化还原分子的还原反应产生的还原电流在样品核酸的扩增反应在缓冲液中进行的条件下，随着样品核酸的扩增进行而形成的焦磷酸 与镁离子形成焦磷酸镁，从而降低缓冲液的镁离子浓度，并根据上述测定的还原电流的大小来确定样品核酸是否已被放大。

    METHOD OF DETECTING A PLURALITY OF NUCLEIC ACIDS
    4.
    发明申请
    METHOD OF DETECTING A PLURALITY OF NUCLEIC ACIDS 审中-公开
    检测多种核酸的方法

    公开(公告)号：US20100021907A1

    公开(公告)日：2010-01-28

    申请号：US12483840

    申请日：2009-06-12

    申请人： Jun OKADA ,  Nobuhiro Gemma

    发明人： Jun OKADA ,  Nobuhiro Gemma

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6837

    摘要： The present invention provides a method of detecting a plurality of nucleic acid samples, includes a first step of preparing a nucleic acid sample detection device, a second step of preparing 1st to nth nucleic acid sample discrimination reagents, a third step of adding the 1st to nth nucleic acid sample discrimination reagents to 1st to nth nucleic acid samples respectively, a fourth step of injecting the 1st to nth nucleic acid samples into 1st to nth wells respectively, a fifth step of detecting the presence or absence of a reaction in positive control immobilization regions in the 1st to nth wells, and a sixth step of detecting the presence or absence of a reaction in detection nucleic acid probe immobilization regions in the 1st to nth wells.

    摘要翻译： 本发明提供检测多个核酸样品的方法，包括制备核酸样品检测装置的第一步骤，制备第1至第n核酸样品鉴别试剂的第二步骤，将第1步骤添加至第1步骤 第n个核酸样品鉴别试剂分别对第1至第n个核酸样品，第4步骤，将第1至第n个核酸样品分别注射到第1至第n个孔中;第五步骤，检测阳性对照固定中存在或不存在反应 第1〜第n孔的检测核酸探针固定区域的反应的存在或不存在的第6工序。

    ORGANIC ELECTROLUMINESCENT ELEMENT AND MANUFACTURING METHOD THEREOF
    6.
    发明申请
    ORGANIC ELECTROLUMINESCENT ELEMENT AND MANUFACTURING METHOD THEREOF 审中-公开
    有机电致发光元件及其制造方法

    公开(公告)号：US20110165704A1

    公开(公告)日：2011-07-07

    申请号：US13040789

    申请日：2011-03-04

    申请人： Kinya KUMAZAWA ,  Jun OKADA ,  Tatsuo MORI

    发明人： Kinya KUMAZAWA ,  Jun OKADA ,  Tatsuo MORI

    IPC分类号： H01L51/40

    CPC分类号： H01L51/5088 ,  H01L51/002 ,  H01L51/0021 ,  H01L51/0037 ,  H01L51/102

    摘要： An organic electroluminescent element 1 according to the present invention includes: a substrate 2; a first electrode 3 formed on the substrate; an organic light-emitting layer 5 formed on the first electrode 3 so as to be brought into contact with the first electrode 3; and a second electrode 6 formed on the organic light-emitting layer 5, characterized in that an ion-doped surface onto which hydrogen ions or hydroxide ions are doped as dopant is provided in the vicinity of a contact interface B between the first electrode 3 and the organic light-emitting layer 5. By such characteristics, an organic electroluminescent element can be obtained, in which a low-voltage drive is made possible, and a long lifetime is realized.

    摘要翻译： 根据本发明的有机电致发光元件1包括：基板2; 形成在基板上的第一电极3; 形成在第一电极3上以与第一电极3接触的有机发光层5; 以及形成在有机发光层5上的第二电极6，其特征在于，在第一电极3和第二电极6之间的接触界面B附近设置掺杂有氢离子或氢氧根离子作为掺杂剂的离子掺杂表面。 有机发光层5.通过这样的特性，可以获得可以实现低电压驱动的有机电致发光元件，并实现长寿命。