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    DIAGNOSTIC CASSETTE FOR ELECTROCHEMICAL MEASURING APPARATUS AND METHOD OF DIAGNOSING ELECTROCHEMICAL MEASURING APPARATUS
    1.
    发明申请
    DIAGNOSTIC CASSETTE FOR ELECTROCHEMICAL MEASURING APPARATUS AND METHOD OF DIAGNOSING ELECTROCHEMICAL MEASURING APPARATUS 审中-公开
    电化学测量仪器诊断仪器及诊断电化学测量仪器的方法

    公开(公告)号：US20100032319A1

    公开(公告)日：2010-02-11

    申请号：US12408980

    申请日：2009-03-23

    申请人： Jun Okada ,  Nobuhiro Gemma ,  Sadato Hongo ,  Tetsuya Kuwabara

    发明人： Jun Okada ,  Nobuhiro Gemma ,  Sadato Hongo ,  Tetsuya Kuwabara

    IPC分类号： G01N27/26

    CPC分类号： G01N27/3277 ,  G01N27/4163

    摘要： A diagnostic cassette for diagnosing an electrochemical measuring apparatus is disclosed. The electrochemical measuring apparatus is to perform electrochemical measurement on a sample in a sample cassette, which is attachable to and detachable from the electrochemical measuring apparatus, and includes a first working electrode interface, a first counter electrode interface, and a first reference electrode interface. The diagnostic cassette is attachable to and detachable from the electrochemical measuring apparatus like the sample cassette, and includes a second working electrode interface connectable to the first working electrode interface, a second counter electrode interface connectable to the first counter electrode interface, a second reference electrode interface connectable to the first reference electrode interface, and one of a capacitor and a resistor including one terminal connected to the second working electrode interface and the other terminal connected to the second counter electrode interface and the second reference electrode interface.

    摘要翻译： 公开了一种用于诊断电化学测量装置的诊断盒。 电化学测量装置是对可附着到电化学测量装置上并可从电化学测量装置拆卸的样品盒中的样品进行电化学测量，并且包括第一工作电极界面，第一对电极界面和第一参考电极界面。 诊断盒可以像样品盒一样安装到电化学测量装置上并可拆卸，并且包括可连接到第一工作电极接口的第二工作电极接口，可连接到第一对电极接口的第二对电极接口，第二参比电极 接口，其可连接到第一参考电极接口，以及电容器和电阻器中的一个，包括连接到第二工作电极接口的一个端子和连接到第二对电极接口和第二参考电极接口的另一端子。

    NUCLEIC ACID DETECTION CASSETTE AND NUCLEIC ACID DETECTION SYSTEM
    2.
    发明申请
    NUCLEIC ACID DETECTION CASSETTE AND NUCLEIC ACID DETECTION SYSTEM 审中-公开
    核酸检测CASSETTE和核酸检测系统

    公开(公告)号：US20080153714A1

    公开(公告)日：2008-06-26

    申请号：US11848623

    申请日：2007-08-31

    申请人： Sadato HONGO ,  Jun Okada ,  Kenji Ooki ,  Nobuhiro Gemma

    发明人： Sadato HONGO ,  Jun Okada ,  Kenji Ooki ,  Nobuhiro Gemma

    IPC分类号： C40B60/10

    CPC分类号： G01N35/1095 ,  G01N33/5308 ,  G01N33/54366

    摘要： A nucleic acid cassette includes a main body in which first and second channels are formed. A sample containing a target nucleic acid is input in a sample chamber of the second channel via an injection port. In the cassette, a detecting part includes a detection chamber communicated to the first and second channels and a nucleic detection unit. The first channel has a first reagent reservoir storing a first reagent and a second reagent reservoir storing a second reagent. The first and second reagents reservoirs are separated by a gaseous body in the channel. The sample and the first and second reagents are supplied to the detecting part due to an action of a pump part communicated to the first and second channels.

    摘要翻译： 核酸盒包括其中形成有第一和第二通道的主体。 含有靶核酸的样品通过注射口输入到第二通道的样品室中。 在盒中，检测部分包括与第一和第二信道通信的检测室和核心检测单元。 第一通道具有存储第一试剂的第一试剂储存器和存储第二试剂的第二试剂储存器。 第一和第二试剂储存器在通道中被气体体分开。 由于与第一和第二通道相通的泵部件的动作，将样品和第一和第二试剂供给到检测部。

    NUCLEIC ACID DETECTION DEVICE
    3.
    发明申请
    NUCLEIC ACID DETECTION DEVICE 有权
    核酸检测装置

    公开(公告)号：US20070227885A1

    公开(公告)日：2007-10-04

    申请号：US11682484

    申请日：2007-03-06

    申请人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    发明人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    IPC分类号： G01N33/487

    CPC分类号： B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0883 ,  G01N27/3275

    摘要： A nucleic acid detection device includes a flow channel through which a solution containing a nucleic acid recognition body flows, probe electrodes having immobilized nucleic acid probes, and a counter electrode used to measure an electrochemical response of the nucleic acid recognition body. The flow channel includes a curved portion and a straight portion continued from and located downstream of the curved portion. The probe electrodes are arranged at intervals along the straight portion, avoiding an upstream end of the straight portion that is located within a distance L from the curved portion. The distance L is given by L=0.065×Re×D, Re=ρuD/μ, and D=4 S/Lp where ρ, u, and μ are a concentration, a flow velocity, and a viscosity of the solution, and S and Lp are a sectional area and a wall peripheral perimeter of the flow channel, respectively.

    摘要翻译： 核酸检测装置包括含有核酸识别体的溶液流过的流路，具有固定化的核酸探针的探针电极和用于测定核酸识别体的电化学响应的对极。 流动通道包括弯曲部分和从弯曲部分连续并位于弯曲部分下游的直线部分。 探针电极沿着直线部分间隔设置，避免了直线部分的距弯曲部分的距离L内的上游端。 距离L由L = 0.065xRexD，Re = rhouD / mu和D = 4S / Lp给出，其中rho，u和μ是溶液的浓度，流速和粘度，S和Lp 分别是流动通道的截面积和壁周边周长。

    NUCLEIC ACID DETECTION DEVICE AND NUCLEIC ACID DETECTION APPARATUS
    4.
    发明申请
    NUCLEIC ACID DETECTION DEVICE AND NUCLEIC ACID DETECTION APPARATUS 审中-公开
    核酸检测装置和核酸检测装置

    公开(公告)号：US20070215466A1

    公开(公告)日：2007-09-20

    申请号：US11555936

    申请日：2006-11-02

    申请人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    发明人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    IPC分类号： G01N33/487

    CPC分类号： G01N27/4473

    摘要： A nucleic acid detection device includes a flow channel which allows a solution containing a nucleic acid recognition body to flow therethrough, at least two first electrode portions including a probe electrode to which at least one nucleic acid probe is immobilized, and at least two second electrode portions including a reference electrode to be used for measuring a reference value. The first and second electrode portions are arranged in the flow channel to form a row along the flow channel. One of the second electrode portions is adjacent to one of the first electrode portions.

    摘要翻译： 核酸检测装置包括允许含有核酸识别体的溶液流过其中的流动通道，至少两个第一电极部分，包括固定有至少一个核酸探针的探针电极和至少两个第二电极 包括用于测量参考值的参考电极的部分。 第一和第二电极部分布置在流动通道中以沿着流动通道形成一排。 第二电极部分中的一个与第一电极部分中的一个相邻。

    Nucleic acid detection device
    5.
    发明授权
    Nucleic acid detection device 有权
    核酸检测装置

    公开(公告)号：US07851206B2

    公开(公告)日：2010-12-14

    申请号：US11682484

    申请日：2007-03-06

    申请人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    发明人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0883 ,  G01N27/3275

    摘要： A nucleic acid detection device includes a flow channel through which a solution containing a nucleic acid recognition body flows, probe electrodes having immobilized nucleic acid probes, and a counter electrode used to measure an electrochemical response of the nucleic acid recognition body. The flow channel includes a curved portion and a straight portion continued from and located downstream of the curved portion. The probe electrodes are arranged at intervals along the straight portion, avoiding an upstream end of the straight portion that is located within a distance L from the curved portion. The distance L is given by L=0.065×Re×D, Re=ρuD/μ, and D=4S/Lp where ρ, u, and μ are a concentration, a flow velocity, and a viscosity of the solution, and S and Lp are a sectional area and a wall peripheral perimeter of the flow channel, respectively.

    摘要翻译： 核酸检测装置包括含有核酸识别体的溶液流过的流路，具有固定化的核酸探针的探针电极和用于测定核酸识别体的电化学响应的对极。 流动通道包括弯曲部分和从弯曲部分连续并位于弯曲部分下游的直线部分。 探针电极沿着直线部分间隔设置，避免了直线部分的距弯曲部分的距离L内的上游端。 距离L由L = 0.065×Re×D，Re =＆rgr; uD /μ和D = 4S / Lp给出，其中＆rgr，u和μ是溶液的浓度，流速和粘度 ，S和Lp分别是流动通道的截面面积和壁周长。

    NUCLEIC ACID DETECTION DEVICE
    6.
    发明申请
    NUCLEIC ACID DETECTION DEVICE 审中-公开
    核酸检测装置

    公开(公告)号：US20080233634A1

    公开(公告)日：2008-09-25

    申请号：US12048690

    申请日：2008-03-14

    申请人： Jun OKADA ,  Sadato Hongo ,  Keiko Ito ,  Kenji Ooki ,  Nobuhiro Gemma

    发明人： Jun OKADA ,  Sadato Hongo ,  Keiko Ito ,  Kenji Ooki ,  Nobuhiro Gemma

    IPC分类号： C12M1/00

    CPC分类号： G01N35/085

    摘要： A nucleic acid detection device is provided with a closed channel formed of a first channel portion, through which a washing reagent storage section for storing a washing reagent for washing a detection section for nucleic acid detection communicates with the detection section, and a second channel portion through which a pretreatment section for nucleic acid treatment communicates with the detection section. The closed channel is connected with a gas inlet/outlet path for communication with the outside. The gas inlet/outlet path is blocked by a sealing mechanism before nucleic acid detection. In storing a pretreatment reagent and the washing reagent frozen, the gas inlet/outlet path is kept open and connected to the channel. Thus, there is provided a nucleic acid detection device having a structure for preventing leakage of nucleic acid samples to the outside and which can be stored for a long period of time with the reagents therein.

    摘要翻译： 核酸检测装置设置有由第一通道部分形成的封闭通道，用于存储用于清洗用于核酸检测的检测部分的洗涤剂的洗涤剂存储部分与检测部分通信，第二通道部分 用于核酸处理的预处理部分与检测部分通信。 封闭通道与用于与外部连通的气体入口/出口路径连接。 在核酸检测之前，气体入口/出口路径被密封机构堵塞。 在储存预处理试剂和洗涤剂冻结时，气体入口/出口路径保持打开并连接到通道。 因此，提供了一种核酸检测装置，其具有防止核酸样品向外部泄漏的结构，并且可以用其中的试剂长时间储存​​。

    DEVICE USED TO DETECT NUCLEIC ACID
    7.
    发明申请
    DEVICE USED TO DETECT NUCLEIC ACID 审中-公开
    用于检测核酸的装置

    公开(公告)号：US20070240985A1

    公开(公告)日：2007-10-18

    申请号：US11734074

    申请日：2007-04-11

    申请人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    发明人： Jun Okada ,  Sadato Hongo ,  Nobuhiro Gemma

    IPC分类号： G01N33/487

    CPC分类号： G01N27/3275 ,  B01L3/5027

    摘要： A device includes a flow channel through which a solution containing at least one of a target nucleic acid and a nucleic acid recognition body flows. The flow channel includes a flat bottom surface parallel to the flowing direction of the solution and an inclined surface gradually projecting from the bottom surface in the flowing direction. The device also includes a probe electrode provided on the inclined surface. The probe electrode is formed of an exposed portion of a metal film through an opening of a passivation film stacked on the metal film. An angle θ that the probe electrode forms with respect to the bottom surface, a maximal width R of the opening in the flowing direction, and a thickness d of the passivation film satisfy tan θ>d/R.

    摘要翻译： 一种装置包括流动通道，含有至少一种靶核酸和核酸识别体的溶液通过该通道流动。 流动通道包括平行于溶液的流动方向的平坦底表面和从流动方向上从底部表面逐渐突出的倾斜表面。 该装置还包括设置在倾斜表面上的探针电极。 探针电极通过层叠在金属膜上的钝化膜的开口由金属膜的暴露部分形成。 探针电极相对于底面形成的角度θ，开口在流动方向上的最大宽度R以及钝化膜的厚度d满足tanθd / R。

    METHOD OF DETECTING NUCLEIC ACID AMPLIFICATION REACTION
    9.
    发明申请
    METHOD OF DETECTING NUCLEIC ACID AMPLIFICATION REACTION 审中-公开
    检测核酸放大反应的方法

    公开(公告)号：US20100099094A1

    公开(公告)日：2010-04-22

    申请号：US12498721

    申请日：2009-07-07

    申请人： Jun OKADA ,  Nobuhiro Gemma

    发明人： Jun OKADA ,  Nobuhiro Gemma

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/682 ,  C12Q1/6825 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2563/113

    摘要： The present invention provides a method of detecting a nucleic acid amplification reaction, including the steps of adding a sample nucleic acid to a nucleic acid amplification buffer containing a reducing agent molecule, a redox molecule and a magnesium ion, to conduct an amplification reaction, measuring a reduction current produced by a reduction reaction of the reducing agent molecule with the redox molecule, under the conditions that when the amplification reaction of the sample nucleic acid has proceeded in the buffer, pyrophosphoric acid formed with the progress of amplification of the sample nucleic acid forms magnesium pyrophosphate with the magnesium ion, thereby decreasing a magnesium ion concentration of the buffer, and determining, from the magnitude of the reduction current measured above, whether the sample nucleic acid has been amplified or not.

    摘要翻译： 本发明提供一种检测核酸扩增反应的方法，包括以下步骤：将样品核酸加入到含有还原剂分子，氧化还原分子和镁离子的核酸扩增缓冲液中进行扩增反应，测定 在还原剂分子与氧化还原分子的还原反应产生的还原电流在样品核酸的扩增反应在缓冲液中进行的条件下，随着样品核酸的扩增进行而形成的焦磷酸 与镁离子形成焦磷酸镁，从而降低缓冲液的镁离子浓度，并根据上述测定的还原电流的大小来确定样品核酸是否已被放大。

    METHOD OF DETECTING A PLURALITY OF NUCLEIC ACIDS
    10.
    发明申请
    METHOD OF DETECTING A PLURALITY OF NUCLEIC ACIDS 审中-公开
    检测多种核酸的方法

    公开(公告)号：US20100021907A1

    公开(公告)日：2010-01-28

    申请号：US12483840

    申请日：2009-06-12

    申请人： Jun OKADA ,  Nobuhiro Gemma

    发明人： Jun OKADA ,  Nobuhiro Gemma

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： C12Q1/6837

    摘要： The present invention provides a method of detecting a plurality of nucleic acid samples, includes a first step of preparing a nucleic acid sample detection device, a second step of preparing 1st to nth nucleic acid sample discrimination reagents, a third step of adding the 1st to nth nucleic acid sample discrimination reagents to 1st to nth nucleic acid samples respectively, a fourth step of injecting the 1st to nth nucleic acid samples into 1st to nth wells respectively, a fifth step of detecting the presence or absence of a reaction in positive control immobilization regions in the 1st to nth wells, and a sixth step of detecting the presence or absence of a reaction in detection nucleic acid probe immobilization regions in the 1st to nth wells.

    摘要翻译： 本发明提供检测多个核酸样品的方法，包括制备核酸样品检测装置的第一步骤，制备第1至第n核酸样品鉴别试剂的第二步骤，将第1步骤添加至第1步骤 第n个核酸样品鉴别试剂分别对第1至第n个核酸样品，第4步骤，将第1至第n个核酸样品分别注射到第1至第n个孔中;第五步骤，检测阳性对照固定中存在或不存在反应 第1〜第n孔的检测核酸探针固定区域的反应的存在或不存在的第6工序。