公开(公告)号：US20120135449A1

公开(公告)日：2012-05-31

申请号：US13365525

申请日：2012-02-03

申请人： Jun Xie ,  Robert John Filkins ,  Fiona Mary Ginty ,  Michael Christopher Montalto ,  Anup Sood ,  Jeffrey Bernard Fortin ,  Wei-Cheng Tian ,  Michael John Gerdes

发明人： Jun Xie ,  Robert John Filkins ,  Fiona Mary Ginty ,  Michael Christopher Montalto ,  Anup Sood ,  Jeffrey Bernard Fortin ,  Wei-Cheng Tian ,  Michael John Gerdes

IPC分类号： G01N21/75 ,  C12M1/34

CPC分类号： G01N1/312

摘要： Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent, and releasing the sample from the flow cell wherein the integrity of the sample is intact. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.

摘要翻译： 提供了促进成像应用中生物样品的迭代染色的自动化方法和装置。 所述方法包括以下步骤：提供含有生物样品的小体积流动池，向生物样品施加染色，将至少两种前体试剂结合以形成活化的脱色剂，并且其中所述活化脱色剂分解速率大于或等于 以脱色反应速率使脱色剂以大于活化脱色剂的分解速率的流速流过生物样品，并将样品从流动池中释放出来，其中样品的完整性是完整的。 染色，组合和流动的过程可以迭代地重复。 本文还公开了用于重复染色生物样品的装置，其包括与预混合器流体连通的流动池，其中预混合器的体积容量小于流动池的体积容量的约五倍。

公开(公告)号：US20090253163A1

公开(公告)日：2009-10-08

申请号：US12061044

申请日：2008-04-02

申请人： Jun Xie ,  Fiona Ginty ,  Robert John Filkins ,  Michael Christopher Montalto ,  Anup Sood ,  Jeffrey Bernard Fortin ,  Wei-Cheng Tian ,  Michael John Gerdes

发明人： Jun Xie ,  Fiona Ginty ,  Robert John Filkins ,  Michael Christopher Montalto ,  Anup Sood ,  Jeffrey Bernard Fortin ,  Wei-Cheng Tian ,  Michael John Gerdes

IPC分类号： G01N1/30 ,  C12M1/34 ,  C12M1/36

CPC分类号： G01N1/312

摘要： Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, and flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell.

摘要翻译： 提供了促进成像应用中生物样品的迭代染色的自动化方法和装置。 所述方法包括以下步骤：提供含有生物样品的小体积流动池，向生物样品施加染色，将至少两种前体试剂结合以形成活化的脱色剂，并且其中所述活化脱色剂分解速率大于或等于 与脱色反应速率相关联，并使脱色剂以大于活化脱色剂分解速率的流速流过生物样品。 染色，组合和流动的过程可以迭代地重复。 本文还公开了用于重复染色生物样品的装置，其包括与预混合器流体连通的流动池，其中预混合器的体积容量小于流动池的体积容量的约五倍。

公开(公告)号：US20100059120A1

公开(公告)日：2010-03-11

申请号：US12208445

申请日：2008-09-11

申请人： Wei-Cheng Tian ,  Jeffrey Bernard Fortin ,  Jun Xie ,  Barbara Grossman ,  Oliver Charles Boomhower ,  Shashi Thutupalli ,  Long Que

发明人： Wei-Cheng Tian ,  Jeffrey Bernard Fortin ,  Jun Xie ,  Barbara Grossman ,  Oliver Charles Boomhower ,  Shashi Thutupalli ,  Long Que

IPC分类号： G01N33/00

CPC分类号： B01L3/502738 ,  B01L3/502707 ,  B01L3/502784 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0874 ,  B01L2300/161 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0475 ,  B01L2400/0655 ,  B01L2400/0672 ,  Y10T137/0318

摘要： Methods and microfluidic devices for generating and manipulating sample droplets, wherein the devices comprise, a plurality of fluid channels, at least one of which is a sample channel for carrying a fluidic sample material, that is in fluid communication with the carrier fluid channel via an orifice; and an actuated flow interrupter adapted to force a predetermined amount of the sample fluid from the sample channel through the orifice into the carrier fluid channel.

摘要翻译： 用于产生和操纵样品液滴的方法和微流体装置，其中所述装置包括多个流体通道，其中至少一个流体通道是用于承载流体样品材料的样品通道，其经由载体流体通道流体连通 孔; 以及致动流动断路器，其适于将预定量的样品流体从样品通道穿过所述孔进入载体流体通道。

公开(公告)号：US20100155244A1

公开(公告)日：2010-06-24

申请号：US12341233

申请日：2008-12-22

申请人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie

发明人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie

IPC分类号： B01D57/02

CPC分类号： B01D57/02

摘要： Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.

摘要翻译： 用于在电泳期间注射样品的方法和系统，其通常包括：通过分离通道的第一端装载筛分基质; 筛分矩阵的结束距离分离通道和加载通道的交点处一定距离; 通过加载通道加载样品并填充分离通道的空的部分; 在使洗涤缓冲液流过所述装载通道的同时，在所述分离通道上施加电场; 以及将所述样品的一部分注入所述分离通道中，其中所述注入的所述样品的所述部分具有由所述筛分矩阵的末端与所述加载和分离通道的交点之间的距离确定的尺寸。

公开(公告)号：US07740747B2

公开(公告)日：2010-06-22

申请号：US11965794

申请日：2007-12-28

申请人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

发明人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

IPC分类号： G01N27/26

CPC分类号： G01N27/44743

摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.

摘要翻译： 提供了用于毛细管电泳的微芯片。 微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。 微芯片还包括邻近'T'结和在分离通道上设置的第一阀和设置在'T'结处的第二阀。 第二个阀是一个二通阀。 将样品塞注入到“T”结和分离通道的第二端之间的区域中。

公开(公告)号：US20090071832A1

公开(公告)日：2009-03-19

申请号：US11857570

申请日：2007-09-19

申请人： Jun Xie ,  Shashi Thutupalli ,  Stacey Joy Kennerly ,  Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Oliver Charles Boomhower

发明人： Jun Xie ,  Shashi Thutupalli ,  Stacey Joy Kennerly ,  Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Oliver Charles Boomhower

IPC分类号： G01N27/26

CPC分类号： G01N27/447

摘要： A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation.

摘要翻译： 提供具有垂直注射孔的微流体装置。 微流体装置包括分离通道，与分离通道相邻并且与分离通道流体连通地设置的注射孔。 微流体装置还包括邻近喷射孔布置的半渗透过滤器，其中过滤器构造成在注射操作期间预浓缩注入孔中的样品以形成预浓缩样品塞，并且其中样品塞从 在电泳操作期间到分离通道的注射孔径。

公开(公告)号：US07927476B2

公开(公告)日：2011-04-19

申请号：US12341233

申请日：2008-12-22

申请人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie

发明人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： B01D57/02

摘要： Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.

摘要翻译： 用于在电泳期间注射样品的方法和系统，其通常包括：通过分离通道的第一端装载筛分基质; 筛分矩阵的结束距离分离通道和加载通道的交点处一定距离; 通过加载通道加载样品并填充分离通道的空的部分; 在使洗涤缓冲液流过所述装载通道的同时，在所述分离通道上施加电场; 以及将所述样品的一部分注入所述分离通道中，其中所述注入的所述样品的所述部分具有由所述筛分矩阵的末端与所述加载和分离通道的交点之间的距离确定的尺寸。

公开(公告)号：US20090166203A1

公开(公告)日：2009-07-02

申请号：US11965794

申请日：2007-12-28

申请人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

发明人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

IPC分类号： G01N27/26 ,  G01N27/447

CPC分类号： G01N27/44743

摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.

摘要翻译： 提供了用于毛细管电泳的微芯片。 微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。 所述微芯片还包括邻近所述“T”结和所述分离通道设置的第一阀，以及设置在所述“T”结处的第二阀。 第二个阀是一个二通阀。 将样品塞注入到“T”结和分离通道的第二端之间的区域中。

公开(公告)号：US20090166202A1

公开(公告)日：2009-07-02

申请号：US11965791

申请日：2007-12-28

申请人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

发明人： Wei-Cheng Tian ,  Erin Jean Finehout ,  Li Zhu ,  Jun Xie ,  Shashi Thutupalli

IPC分类号： B01D57/02

CPC分类号： G01N27/44743 ,  G01N27/44791

摘要： A microchip for electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well. The injection channel and the separation channel form a ‘T’ junction. The microchip comprises a first electrode disposed at a first end of the separation channel, a second electrode disposed in front of the ‘T’ junction and adjacent to the first electrode, a third electrode disposed at a first end of the injection channel and a fourth electrode disposed at a second end of the separation channel. A portion of the sample is injected and separated into an area between the ‘T’ junction and the fourth electrode.

摘要翻译： 提供用于电泳的微芯片。 微芯片包括注入通道和配置成通过样品阱接收样品的分离通道。 注入通道和分离通道形成“T”结。 微芯片包括设置在分离通道的第一端的第一电极，设置在“T”结的前面并与第一电极相邻的第二电极，设置在注入通道的第一端的第三电极和第四电极 电极设置在分离通道的第二端。 样品的一部分被注入并分离成“T”结和第四电极之间的区域。

公开(公告)号：US07740748B2

公开(公告)日：2010-06-22

申请号：US12258657

申请日：2008-10-27

申请人： Wei-Cheng Tian ,  Shashi Thutupalli ,  Li Zhu ,  Anthony John Murray ,  Erin Jean Finehout ,  Jun Xie

发明人： Wei-Cheng Tian ,  Shashi Thutupalli ,  Li Zhu ,  Anthony John Murray ,  Erin Jean Finehout ,  Jun Xie

IPC分类号： G01N27/447 ,  G01N27/453

CPC分类号： B01D57/02 ,  B01L3/502715 ,  G01N27/44743

摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir.

摘要翻译： 电泳系统和方法包括电泳装置，其中电泳装置包括装载通道，注射通道和分离通道。 装载通道与第一和第二采样端口流体连通。 注入通道连接到装载通道以形成第一交叉。 分离通道连接到注入通道以形成第二交叉并且与第一和第二储存器流体连通，并且其中注入通道与第三储存器流体连通。 电泳系统还包括分别耦合到第一样品端口和第三储存器的电极，以及分别适于将样品移动到装载通道中的第一储存器和第二储存器，以朝向第三储存器形成样品塞 并且将样品塞进一步朝向第二储存器移动到分离通道中。