公开(公告)号：US4803154A

公开(公告)日：1989-02-07

申请号：US856018

申请日：1986-04-25

申请人： Junko Uo ,  Masaji Nishimura ,  Kazuo Horiguchi ,  Setsuko Nakanishi ,  Shigeru Kaku

发明人： Junko Uo ,  Masaji Nishimura ,  Kazuo Horiguchi ,  Setsuko Nakanishi ,  Shigeru Kaku

IPC分类号： G01N33/543 ,  G01N33/535 ,  G01N33/545 ,  G01N33/53

CPC分类号： G01N33/545 ,  G01N33/535 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/809

摘要： An assay for an immunologically active substance (B) in a biologically derived test sample comprising using an immuno-active membrane of a hydrophobic polymer sheet having numerous hydrophilic spots, on which a prefixed amount of an immuno-active substance (A) or (B) has been immobilized, and a coloring-membrane having numerous spots which contain a color-producing reagent being capable of developing color by the catalytic effect of an enzyme at the corresponding positions to those of the immuno-active membrane, and making contact the immuno-active membrane with said test sample in the presence of an enzyme-labeled immuno-active substance (A') or (B') and a substrate for reacting the substance (A), substance (B) and enzyme-labeled substance (A') or (B') competitively or non-competitively, and superposing the coloring-membrane of on the immuno-active membrane, followed by colorimetrically measuring the resulting color and thereby determining the amount of the substance (B) in the test sample.

摘要翻译： 在生物衍生的测试样品中的免疫活性物质（B）的测定法，其包括使用具有许多亲水点的疏水性聚合物片的免疫活性膜，其上预定量的免疫活性物质（A）或（B ）已经被固定，并且具有许多斑点的着色膜，其含有通过在与免疫活性膜的位置相对应的位置处的酶的催化作用而能够显色的着色试剂，并且接触免疫 在酶标记的免疫活性物质（A'）或（B'）和用于使物质（A），物质（B）和酶标记物质（A） '）或（B'）竞争性或非竞争性，并且将免疫活性膜上的着色膜叠加，然后通过比色测量所得颜色，从而确定测试中物质（B）的量 充足。