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公开(公告)号：US20060160688A1
公开(公告)日：2006-07-20
申请号：US11333857
申请日：2006-01-17
申请人： Kak Namkoong , Young-sun Lee , Kwang-wook Oh , Christopher Ko , Jung-Im Han
发明人： Kak Namkoong , Young-sun Lee , Kwang-wook Oh , Christopher Ko , Jung-Im Han
IPC分类号： B04B5/02
CPC分类号： B01L3/5021 , B01L2300/043 , B01L2300/0681 , B04B5/0414 , B04B9/00 , B04B9/12
摘要： A handheld centrifuge is provided. In the handheld centrifuge, an inertia body has an axle to which one end of a string is connected and a pair of inertia wheels coupled to the axle, and at least one closed vessel is installed at the inertia body to contain a substance to be centrifuged.
摘要翻译： 提供手持离心机。 在手持式离心机中，惯性体具有一个轴的一端连接到该轴上，一对惯性轮联接到轴上，并且至少一个密封容器安装在惯性体上以容纳要离心的物质 。
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公开(公告)号：US07517690B2
公开(公告)日：2009-04-14
申请号：US11291053
申请日：2005-11-30
申请人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
发明人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
IPC分类号： G01N33/48
CPC分类号： C12N1/06 , Y10T436/107497 , Y10T436/206664 , Y10T436/25 , Y10T436/2575
摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).
摘要翻译： 提供了使用自由基裂解细胞或病毒的方法。 该方法包括：向金属离子，过氧化物和细胞或病毒溶液的混合物施加电场以增加自由基产生，从而裂解细胞或病毒。 在本方法中，可以使用低电能（几mV至几V）有效地进行细胞裂解。 当本方法应用于微系统时，细胞裂解可以通过控制电能在期望的时间和期望的空间中发生，从而适于实现芯片上的实验室（LOC）。
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公开(公告)号：US20060115902A1
公开(公告)日：2006-06-01
申请号：US11291053
申请日：2005-11-30
申请人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
发明人： Jung-im Han , Young-sun Lee , Kak Namkoong , Kwang-wook Oh
IPC分类号： G01N1/10
CPC分类号： C12N1/06 , Y10T436/107497 , Y10T436/206664 , Y10T436/25 , Y10T436/2575
摘要： Provided is a method of lysing a cell or a virus using a free radical. The method includes: applying an electric field to a mixture of a metal ion, a peroxide, and a cell or virus solution to increase the free radical generation, thereby lysing a cell or a virus. In the present method, cell lysis may be efficiently performed using a low electrical energy (several mV to several V). When the present method is applied to a microsystem, cell lysis can occur at a desired time and in a desired space by controlling the electrical energy, thus being suitable to realize a lab-on-a-chip (LOC).
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公开(公告)号：US07579151B2
公开(公告)日：2009-08-25
申请号：US11191221
申请日：2005-07-27
申请人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
发明人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
CPC分类号： C12N15/1006
摘要： Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.
摘要翻译： 提供了使用水凝胶分离和纯化生物分子的方法，该方法包括：使含有带电生物分子的样品与水凝胶接触以将生物分子结合至水凝胶; 洗涤与生物分子结合的水凝胶; 并使用洗脱溶剂洗脱结合的生物分子。 根据该方法，使用具有大表面积的水凝胶将生物分子的隔离时间减少到5分钟以下，不需要诸如电磁体的外部装置，并且可以容易地实现小尺寸系统或LOC 通过聚合物图案化技术适用于微系统。
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公开(公告)号：US07645223B2
公开(公告)日：2010-01-12
申请号：US11333857
申请日：2006-01-17
CPC分类号： B01L3/5021 , B01L2300/043 , B01L2300/0681 , B04B5/0414 , B04B9/00 , B04B9/12
摘要： A handheld centrifuge includes an inertia body having an axle and a pair of inertia wheels coupled to the axle. The inertia wheels are coupled to the axle in substantially perpendicular direction to the axle, and spaced apart from each other. A string is connected to the axle. At least one closed vessel is detachably installed on an outer face of the inertia wheel to contain a substance to be centrifuged. The closed vessel is installed in substantially radial direction of the inertia wheel.
摘要翻译： 手持式离心机包括惯性体，其具有轴和耦合到该轴的一对惯性轮。 惯性轮以基本上垂直于轴的方向联接到车轴并且彼此间隔开。 一根绳子连接到轴上。 至少一个密封容器可拆卸地安装在惯性轮的外表面上以容纳待离心的物质。 密闭容器沿惯性轮的大致径向安装。
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公开(公告)号：US20060127887A1
公开(公告)日：2006-06-15
申请号：US11191221
申请日：2005-07-27
申请人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
发明人： Young-sun Lee , Jung-im Han , Hyo-yeon Lee , Kak Namkoong , Kwang-wook Oh
CPC分类号： C12N15/1006
摘要： Provided is a method of isolating and purifying biomolecules using a hydrogel, the method including: bring a sample containing charged biomolecules into contact with a hydrogel to bind the biomolecules to the hydrogel; washing the hydrogel bound with the biomolecules; and eluting the bound biomolecules using an elution solvent. According to the method, the use of a hydrogel with a large surface area reduces the isolation time of biomolecules to 5 min or less, an external device such as an electromagnet is not required, and small-sized systems or LOC can be easily implemented due to applicability to microsystems through a polymer patterning technique.
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公开(公告)号：US20060169339A1
公开(公告)日：2006-08-03
申请号：US11346452
申请日：2006-02-02
申请人： Kwang-wook Oh , Young-sun Lee , Jung-im Han
发明人： Kwang-wook Oh , Young-sun Lee , Jung-im Han
IPC分类号： F15C1/04
CPC分类号： F16K99/0003 , B01L3/502738 , B01L2300/0861 , B01L2400/0622 , B01L2400/0672 , F16K99/0001 , F16K99/0034 , F16K99/0042 , F16K2099/0069 , F16K2099/0074 , F16K2099/0084 , Y10T137/2191
摘要： A microvalve device is provided. The microvalve device uses electrolysis and uses a hydrogel swelling and deswelling in response to anions or cations as an actuator for controlling the path of a flowing fluid. The microvalve device does not require a buffer solution but uses the transfer fluid flowing in a valve as a source driving the actuator. To generate the anions or cations, an electrode is needed for electrolysis of the fluid near the hydrogel. The microvalve is easy to manufacture and has a simple structure. In addition, the micro valve is useful to manufacture fluid channel arrays having various multi-channel structures.
摘要翻译： 提供微型阀装置。 微阀装置使用电解，并且响应于阴离子或阳离子作为用于控制流动流体的路径的致动器，使用水凝胶溶胀和溶解。 微阀装置不需要缓冲溶液，而是使用流过阀的转移流体作为驱动致动器的源。 为了产生阴离子或阳离子，需要电极来电解水凝胶附近的流体。 微型阀门易于制造，结构简单。 此外，微阀可用于制造具有各种多通道结构的流体通道阵列。
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公开(公告)号：US20090170189A1
公开(公告)日：2009-07-02
申请号：US12184317
申请日：2008-08-01
申请人： Chin-sung PARK , Kak Namkoong , Young-sun Lee
发明人： Chin-sung PARK , Kak Namkoong , Young-sun Lee
CPC分类号： C12M23/16 , B01L2200/12 , B01L2300/0816 , B01L2300/1827 , B29C65/02 , B29C65/08 , B29C66/1122 , B29C66/30223 , B29C66/47 , B29C66/53461 , B29C66/61 , B29C66/71 , B29C66/7352 , B29C66/73921 , B29C66/8322 , B29K2023/06 , B29K2023/12 , B29K2033/12 , B29K2067/00 , B29K2069/00 , B29L2031/756 , Y10T156/10 , B29K2067/003 , B29K2023/38
摘要： Provided are a microfluidic device that performs a biochemical reaction using a small amount of a biochemical fluid and detects the result thereof, and a method of fabricating the same. The microfluidic device includes: a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and a film welded on the bottom surface of the substrate to seal the chamber so that the chamber is not open at the bottom surface of the substrate, and formed of polymer. The method of fabricating a microfluidic device includes: preparing a substrate which comprises a chamber that is formed as a concave groove and accommodates a fluid in the bottom surface of the substrate, and is formed of polymer; and welding a film on a bottom surface of the substrate to seal the chamber so that the chamber is not opened at the bottom surface of the substrate, the film being formed of polymer.
摘要翻译： 提供了使用少量生物化学流体进行生化反应并检测其结果的微流体装置及其制造方法。 微流体装置包括：基板，其包括形成为凹槽并容纳基板的底表面中的流体的腔室，并且由聚合物形成; 以及焊接在基板的底表面上的膜以密封室，使得室不在基板的底表面处开口，并且由聚合物形成。 制造微流体装置的方法包括：制备基板，其包括形成为凹槽的腔室，并且在基底的底表面容纳流体，并且由聚合物形成; 并且在所述基板的底表面上焊接膜以密封所述室，使得所述室在所述基板的底表面处不被打开，所述膜由聚合物形成。
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公开(公告)号：US20060047443A1
公开(公告)日：2006-03-02
申请号：US11217694
申请日：2005-09-01
申请人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
发明人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
CPC分类号： C12Q1/6851
摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.
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10.发明授权公开(公告)号：US08386191B2
公开(公告)日：2013-02-26
申请号：US13161788
申请日：2011-06-16
申请人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
发明人： Kak Namkoong , Jin-tae Kim , Young-sun Lee , Young-a Kim
IPC分类号： G01N33/48
CPC分类号： C12Q1/6851
摘要： Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.
摘要翻译： 提供了从实时核酸扩增数据定量核酸的初始浓度的方法。 使用酶扩增从生物或病毒提取的核酸（DNA或RNA）。 然后，通过计算由核酸的背景荧光强度减去的核酸的荧光强度具有其最大值的一半的特征扩增循环数或特异性扩增时间来发现核酸的初始浓度，或 扩增效率具有由核酸的背景荧光强度减去的核酸的最大值或最小值或以前的扩增荧光强度的特征扩增循环数或特征扩增时间。 因此，可以在不分化或整合的情况下计算核酸的初始浓度。
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