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1.发明授权公开(公告)号：US08129511B2
公开(公告)日：2012-03-06
申请号：US13023061
申请日：2011-02-08
申请人： Karsten Matthias Kragh , Bo Spange Sørensen , Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Gijsbert Gerritse , Wei Liu , Andrew Shaw
发明人： Karsten Matthias Kragh , Bo Spange Sørensen , Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Gijsbert Gerritse , Wei Liu , Andrew Shaw
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This invention relates to nucleic acids encoding amylase polypeptides, the encoded polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exo-specificity and/or thermostability. Some embodiments of the invention relate to said polypeptides and nucleic acids and their uses as non-maltogenic exoamylases in producing food products.
摘要翻译： 本发明涉及编码淀粉酶多肽的核酸，编码的多肽及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地，本发明的淀粉酶显示出改变的外部特异性和/或热稳定性。 本发明的一些实施方案涉及所述多肽和核酸及其在制备食品中的非麦芽糖胞外淀粉酶的用途。
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2.发明授权Thermostable amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权热稳定淀粉酶多肽，编码那些多肽的核酸及其用途公开(公告)号：US07833770B2
公开(公告)日：2010-11-16
申请号：US11528999
申请日：2006-09-27
申请人： Karsten Matthias Kragh , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Carol Fioresi , Gijsbert Gerritse , Wei Liu , Andrew Shaw
发明人： Karsten Matthias Kragh , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Carol Fioresi , Gijsbert Gerritse , Wei Liu , Andrew Shaw
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This is invention relates to amylase polypeptides, and nucleic acids encoding the polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.
摘要翻译： 本发明涉及淀粉酶多肽，以及编码多肽的核酸及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地说，本发明的淀粉酶显示出改变的热稳定性。
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3.发明授权Thermostable amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权热稳定淀粉酶多肽，编码那些多肽的核酸及其用途公开(公告)号：US07776576B2
公开(公告)日：2010-08-17
申请号：US10886905
申请日：2004-07-07
申请人： Karsten Matthias Kragh , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
发明人： Karsten Matthias Kragh , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Oene R. Veltman , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
CPC分类号： C12N9/2417 , A21D8/042 , A23L29/06 , A23L29/35 , A23L33/17 , A23V2002/00 , A23V2300/21
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.
摘要翻译： 本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地说，本发明的淀粉酶显示出改变的热稳定性。
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4.发明授权Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权特异性淀粉酶多肽，编码那些多肽的核酸及其用途公开(公告)号：US08143048B2
公开(公告)日：2012-03-27
申请号：US10886903
申请日：2004-07-07
申请人： Karsten Matthias Kragh , Kirsten Bojsen , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
发明人： Karsten Matthias Kragh , Kirsten Bojsen , Casper Tune Berg , Patrick M. F. Derkx , Anja H. Kellett-Smith , Charlotte Refdahl Thoudahl , Carol Fioresi , Wei Liu , Andrew Shaw , Gijsbert Gerritse
CPC分类号： C12N9/2417
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.
摘要翻译： 本发明涉及淀粉酶多肽和编码多肽的核酸及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地说，本发明的淀粉酶表现出改变的促进作用。
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5.发明授权Modified amylases from pseudomonas species, methods of making and uses thereof 有权来自假单胞菌种的修饰的淀粉酶，其制备方法和用途公开(公告)号：US08137944B2
公开(公告)日：2012-03-20
申请号：US11887977
申请日：2005-07-07
申请人： Casper Tune Berg , Patrick M. F. Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sørensen , Charlotte Refdahl Thoudahl
发明人： Casper Tune Berg , Patrick M. F. Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sørensen , Charlotte Refdahl Thoudahl
IPC分类号： C12N9/28 , C12N9/26 , C12N9/24 , C12N9/00 , C12P21/06 , A21D2/00 , A23C17/00 , A23L1/08 , A23K3/00 , A23K3/08 , C07H21/04 , C07K1/00
CPC分类号： C12Y302/0106 , A21D8/042 , A23K20/189 , A23L7/107 , A23L29/35 , A23V2002/00 , C12N9/2417 , A23V2300/21
摘要： Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophila exoamylase sequence shown as SEQ ID NO: 1.
摘要翻译： 衍生自具有非麦芽糖外切酶活性的亲本多肽的变体多肽，其中所述变体多肽在选自以下的一个或多个位置包含氨基酸突变：121,161,223,146,157,158,199， 229,303,306,309,316,353,26,70,105,188,272,339，参考如SEQ ID NO：1所示的糖酵母假单胞菌外切酶序列的位置编号。
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6.发明授权Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权特异性淀粉酶多肽，编码那些多肽的核酸及其用途公开(公告)号：US08809032B2
公开(公告)日：2014-08-19
申请号：US12024324
申请日：2008-02-01
申请人： Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Charlotte Refdahl Thoudahl , Anja Hemmingsen Kellett-Smith
发明人： Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Charlotte Refdahl Thoudahl , Anja Hemmingsen Kellett-Smith
CPC分类号： C12N9/2417
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically the amylases of the current invention show an altered exospecifity.
摘要翻译： 本发明涉及淀粉酶多肽，以及编码多肽的核酸及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地说，本发明的淀粉酶表现出改变的特异性。
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公开(公告)号：US06607904B2
公开(公告)日：2003-08-19
申请号：US10043142
申请日：2002-01-14
IPC分类号： C12N990
摘要： The present invention provides a method for producing a secretable polypeptide in a host cell. In the method, a peptidyl prolyl cis-trans isomerase is overexpressed in a host cell, thereby increasing the yield of the secreted polypeptide.
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公开(公告)号：US06638737B1
公开(公告)日：2003-10-28
申请号：US09806399
申请日：2001-08-01
IPC分类号： C12P2100
摘要： The present invention provides a method for producing a secretable polypeptide in a host cell. In the method, a peptidyl prolyl cis-trans isomerase is overexpressed in a host cell, thereby increasing the yield of the secreted polypeptide.
摘要翻译： 本发明提供了在宿主细胞中产生可分泌多肽的方法。 在该方法中，肽基脯氨酰顺反异构酶在宿主细胞中过表达，从而增加分泌的多肽的产量。
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