公开(公告)号：US5783450A

公开(公告)日：1998-07-21

申请号：US440359

申请日：1995-05-12

申请人： Kasumi Yoshida ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Masahito Ito ,  Masao Kamahori

发明人： Kasumi Yoshida ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Masahito Ito ,  Masao Kamahori

IPC分类号： G01N30/06 ,  G01N21/64 ,  G01N30/08 ,  G01N30/74 ,  G01N30/88 ,  G01N33/88 ,  G01N35/00 ,  G01N35/04 ,  G01N35/10 ,  G01N30/02

CPC分类号： G01N35/0092 ,  G01N30/88 ,  G01N2030/067 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2035/00356 ,  G01N2035/0093 ,  G01N2035/106 ,  G01N21/64 ,  G01N30/06 ,  G01N35/028 ,  G01N35/109 ,  Y10T436/142222 ,  Y10T436/173845 ,  Y10T436/25375

摘要： A method and an instrument for separating and analyzing a very small quantity of constituents to be inspected contained in an analyte such as a class of catecholamines by liquid chromatography after labeling. The entire processes of analytical operations include principally three processes, a preparing process, an impurity elimination process and a separation process. When one sample is perceived, the sample is processed in these three processes. In this case, while one sample is being processed in an impurity elimination process, a sample preceeding the one sample is processed in a separation process, and a succeeding sample is processed in a preparing process. In other words, these three processes are performed essentially in parallel. With this, it is possible to remarkably reduce analysis time as compared with individual single sample analysis.

摘要翻译： 一种方法和仪器，用于通过液相色谱法在标记后通过液相色谱法分离和分析非常少量待检查的成分，包含在分析物如一类儿茶酚胺中。 分析操作的整个过程主要包括三个过程，制备过程，杂质消除过程和分离过程。 当一个样品被感知时，样品在这三个过程中被处理。 在这种情况下，当在杂质消除过程中处理一个样品时，在分离过程中处理先于一个样品的样品，并在制备过程中处理后续样品。 换句话说，这三个过程基本上是并行执行的。 与单个样品分析相比，可以显着减少分析时间。

公开(公告)号：US5804142A

公开(公告)日：1998-09-08

申请号：US756430

申请日：1996-11-26

申请人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

发明人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

IPC分类号： G01N30/86 ,  G01N30/62 ,  G01N30/88 ,  G01N30/02

CPC分类号： G01N30/88 ,  G01N2030/626 ,  G01N2030/8804

摘要： A reference sample having a known concentration is injected into a chromatograph system before an unknown sample is analyzed. This is for the purpose of checking the deterioration of system performance or in other words for the purpose of checking system suitability. In the present invention, the chromatographic characteristic of the check sample at the time of deterioration of the system suitability is expected and stored in a memory in advance to be related to factors of deterioration of system suitability. A factor of deterioration is deduced by comparison between the actually analyzed chromatographic characteristic of the check sample and the relational data stored in the memory. A guidance required for removing the factor is displayed. Disclosed are preferred embodiments of a chromatograph system suitable for analyzing catecholamine or glycated hemoglobin.

摘要翻译： 在分析未知样品之前，将具有已知浓度的参考样品注入色谱系统中。 这是为了检查系统性能的恶化，换句话说是为了检查系统适用性。 在本发明中，期望在系统适用性恶化时检查样品的色谱特性，并预先存储在存储器中，以与系统适用性的劣化因素相关。 通过比较检查样品的实际分析色谱特征和存储在存储器中的关系数据，推导出劣化因素。 显示删除因子所需的指导。 公开了适用于分析儿茶酚胺或糖化血红蛋白的色谱系统的优选实施方案。

公开(公告)号：US5308774A

公开(公告)日：1994-05-03

申请号：US625823

申请日：1990-12-11

申请人： Junkichi Miura ,  Masahito Ito ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

发明人： Junkichi Miura ,  Masahito Ito ,  Yoshio Fujii ,  Hiroshi Satake ,  Kasumi Yoshida

IPC分类号： G01N30/06 ,  G01N21/64 ,  G01N30/04 ,  G01N30/14 ,  G01N30/24 ,  G01N30/74 ,  G01N30/88 ,  G01N35/00 ,  G01N35/04 ,  G01N35/10 ,  G01N33/88

CPC分类号： G01N30/24 ,  G01N35/00 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2035/00356 ,  G01N2035/00435 ,  G01N2035/1032 ,  G01N21/64 ,  G01N30/74 ,  G01N35/028 ,  G01N35/1004 ,  G01N35/109 ,  Y10T436/173845 ,  Y10T436/25125 ,  Y10T436/25625

摘要： Physiologically active substances contained in a biological sample not subjected to deproteinization can be converted to their derivatives at a high efficiency without being adversely affected by the protein present in the sample, and accordingly the analysis of said physiologically active substances in the form of said derivatives can be performed at a high precision.

摘要翻译： 未经脱蛋白化的生物样品中含有的生理活性物质可以高效率地转化为其衍生物，而不受样品中存在的蛋白质的不利影响，因此所述衍生物形式的所述生理活性物质的分析可以 以高精度执行。

公开(公告)号：US5277871A

公开(公告)日：1994-01-11

申请号：US5310

申请日：1993-01-19

申请人： Yoshio Fujii ,  Kasumi Yoshida ,  Junkichi Miura ,  Hiroshi Satake ,  Masahito Ito

发明人： Yoshio Fujii ,  Kasumi Yoshida ,  Junkichi Miura ,  Hiroshi Satake ,  Masahito Ito

IPC分类号： G01N21/64 ,  G01N30/06 ,  G01N30/88 ,  G01N35/00 ,  G01N35/10 ,  B01D15/08

CPC分类号： G01N35/0092 ,  G01N30/88 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2035/00356 ,  G01N2035/00435 ,  G01N2035/0093 ,  G01N2035/106 ,  G01N21/64 ,  G01N30/06 ,  G01N35/1004 ,  G01N35/109 ,  Y10T436/25625 ,  Y10T436/2575

摘要： The present invention provides a liquid chromatographic analyzer for analyzing a sample containing components for analysis by reacting the sample with a pre-labeling reagent to obtain a reaction mixture containing labeled components, concentrating the reaction mixture and separating the labeled components from each other, and detecting the separated labeled components by a detector, the steps of analysis being controlled to concurrently proceed by a control unit, thereby shortening the time for analysis per sample which analyzer includes: (a) a unit for storing samples containing components for analysis and a pre-labeling reagent for the components at a constant temperature, (b) a unit for reacting the sample with the pre-labeling reagent at a constant temperature to obtain a reaction mixture containing labeled components, (c) a unit for transferring one of the samples and the pre-labeling reagent from unit (a) to unit (b), (d) a unit for concentrating the reaction mixture and separating the labeled components from each other, (e) a unit for detecting the separated labeled components, and (f) a unit for controlling the steps of analysis to concurrently proceed, thereby shortening the time for analysis per sample.

摘要翻译： 本发明提供一种液相色谱分析仪，用于通过使样品与预标记试剂反应来分析含有分析成分的样品，以获得含有标记组分的反应混合物，浓缩反应混合物并将标记的组分彼此分离，并检测 通过检测器分离的标记的成分，分析的步骤被控制以由控制单元同时进行，从而缩短每个样品的分析时间，该分析仪包括：（a）用于存储包含用于分析的组分的样品的单元， （b）用于使样品与预标记试剂在恒定温度下反应以获得含有标记组分的反应混合物的单元，（c）用于转移样品之一的单元和 单元（a）至单位（b）的预标记试剂，（d）用于浓缩反应混合物的单元和分离 （e）用于检测分离的标记成分的单元，和（f）用于控制分析步骤同时进行的单元，从而缩短每个样品的分析时间。

公开(公告)号：US5670379A

公开(公告)日：1997-09-23

申请号：US372708

申请日：1995-01-13

申请人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Mitsuo Ito ,  Fuminori Umesato, deceased

发明人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Mitsuo Ito ,  Fuminori Umesato, deceased

IPC分类号： G01N30/86 ,  G01N30/02 ,  G01N30/62 ,  G01N30/82 ,  G01N30/88

CPC分类号： G01N30/82 ,  G01N30/88 ,  G01N2030/027 ,  G01N2030/625 ,  G01N2030/626 ,  G01N2030/8804 ,  G01N30/8631

摘要： A chromatograph system with improved automation is provided. In the chromatograph system of one embodiment, a regression line is set between retention times of predetermined peaks measured at each run in the past for a standard sample having known components. Referring to this regression line, the peak identifying condition, i.e., time window, is corrected. In another embodiment, there is provided a chromatograph apparatus having a function of estimating a limited life time of a system component. The analyzed results of the chromatograph system can thus be improved.

摘要翻译： 提供了自动化程度提高的色谱系统。 在一个实施方案的色谱系统中，对于具有已知组分的标准样品，在过去每次运行时测量的预定峰的保留时间之间设置回归线。 参考该回归线，校正峰识别条件，即时间窗。 在另一个实施方案中，提供了具有估计系统组分的有限寿命的功能的色谱仪。 因此可以提高色谱系统的分析结果。

公开(公告)号：US5436166A

公开(公告)日：1995-07-25

申请号：US180472

申请日：1994-01-12

申请人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Fuzio Yamada ,  Kouichi Tagami ,  Fuminori Umesato

发明人： Masahito Ito ,  Junkichi Miura ,  Yoshio Fujii ,  Hiroshi Satake ,  Fuzio Yamada ,  Kouichi Tagami ,  Fuminori Umesato

IPC分类号： G01N30/86 ,  G01N1/00 ,  G01N21/64 ,  G01N30/24 ,  G01N30/74 ,  G01N30/88 ,  G01N35/10 ,  B01D15/08

CPC分类号： G01N35/1097 ,  G01N2030/047 ,  G01N2030/8804 ,  G01N21/64 ,  G01N30/24 ,  G01N30/74 ,  G01N30/8631 ,  G01N30/8658 ,  G01N30/8668 ,  G01N35/109

摘要： The present invention relates to a chromatography analysis method and a system employing the same which are capable of automatically determining an constituents to be detected contained in the unknown sample based on results on measuring a standard sample. The standard sample is first separated to obtain its chromatogram. The system extracts the necessary information from the chromatogram to identify the peaks corresponding to the constituents to be detected to set widths of time windows for the peaks. Then, the time windows thus set are applied to the chromatograms obtained by the separation of the unknown sample to identify the constituents contained in the unknown sample. By employing the present invention, it is unnecessary for an operator to input the retention time of each constituent to the system.

摘要翻译： 本发明涉及一种色谱分析方法及其采用该方法的系统，其基于测量标准样品的结果能够自动确定未知样品中包含的待检测成分。 首先分离标准样品以获得其色谱图。 系统从色谱图中提取必要的信息，以识别与要检测的成分相对应的峰以设置峰的时间窗宽度。 然后，将如此设置的时间窗应用于通过分离未知样品获得的色谱图，以鉴定未知样品中所含的成分。 通过采用本发明，操作者不必将每个成分的保留时间输入到系统。

公开(公告)号：US5212097A

公开(公告)日：1993-05-18

申请号：US616246

申请日：1990-11-20

申请人： Masao Kamahori ,  Mamoru Taki ,  Junkichi Miura ,  Taro Nogami ,  Yoshio Watanabe

发明人： Masao Kamahori ,  Mamoru Taki ,  Junkichi Miura ,  Taro Nogami ,  Yoshio Watanabe

IPC分类号： G01N30/06 ,  G01N30/24 ,  G01N30/54 ,  G01N30/74 ,  G01N30/88 ,  G01N33/58 ,  G01N33/94

CPC分类号： G01N33/58 ,  G01N33/9453 ,  G01N2030/8804 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N30/24 ,  G01N30/54 ,  G01N30/74 ,  Y10T436/173845 ,  Y10T436/203332

摘要： Catecholamines in a living body fluid are analyzed by a process which involves reacting a living body fluid sample with a boron compound of the formula: ##STR1## wherein R is hydroxyl, aryl or alkyl, to form a complex of the boron compound with a catecholamine; deproteinizing the reaction solution containing the complex; decomposing the complex to release the catecholamine; labeling the released catecholamine to form a labeled catecholamine; eluting the labeled catecholamine adsorbed on a separation column; and detecting the eluted, labeled catecholamine. Advantageously, this process enables catecholamines to be measured with a high recovery ratio and high accuracy by forming the catecholamines into a complex with a boron compound.

公开(公告)号：US4823168A

公开(公告)日：1989-04-18

申请号：US116543

申请日：1987-11-04

申请人： Masao Kamahori ,  Yoshio Watanabe ,  Junkichi Miura ,  Mamoru Taki ,  Hiroyuki Miyagi

发明人： Masao Kamahori ,  Yoshio Watanabe ,  Junkichi Miura ,  Mamoru Taki ,  Hiroyuki Miyagi

IPC分类号： G01N21/05 ,  G01N30/74

CPC分类号： G01N21/05 ,  G01N2021/0346 ,  G01N2021/054 ,  G01N2030/746 ,  G01N30/74

摘要： A flow cell for a photometer, which comprises a cell body integrated from a pair of cell body members by joining, at least one of which is provided with a linear groove on the joining surface of the cell body member from one end to another to the full length, as exposed to the joining surface, the groove playing both roles of a liquid sample passage and a detecting light path by the integration of the cell body members, and is further provided with a liquid sample inlet passage at a position near one end of the groove and a liquid sample outlet passage at a position near the other end of the groove, both passages being communicated with the grooves, the linear groove being provided with a light reflecting layer to the full length of the linear groove, and a pair of light transmission window members joined with the flow cell body at both ends on the groove-open sides thereof is provided, where polishing of the grooves is readily carried out and random reflection of light or absorption of light due to poor reflection is reduced, and the turbulent flow of a liquid sample and generation of noise are suppressed.

摘要翻译： 一种用于光度计的流动池，其包括通过接合从一对电池主体部件集成的电池体，其中至少一个在电池体部件的接合表面上从一端到另一端设置有线性槽 全长，暴露于接合表面，沟槽通过集成电池主体部件而兼容液体样品通道和检测光路两者，并且在靠近一端的位置处进一步设置有液体样品入口通道 的槽和在槽的另一端附近的位置处的液体样品出口通道，两个通道与槽连通，所述直线槽在直线槽的整个长度上设置有光反射层，以及一对 提供了在其开槽侧的两端与流通池本体连接的透光窗构件，其中容易进行槽的抛光和光或吸收的随机反射o 由于反射不良导致的光衰减，液体试样的紊流和噪声的产生被抑制。

公开(公告)号：US5093267A

公开(公告)日：1992-03-03

申请号：US267293

申请日：1988-11-04

申请人： Junkichi Miura ,  Mamoru Taki ,  Yoshio Watanabe ,  Masao Kamahori ,  Hiroyuki Miyagi

发明人： Junkichi Miura ,  Mamoru Taki ,  Yoshio Watanabe ,  Masao Kamahori ,  Hiroyuki Miyagi

IPC分类号： G01N30/06 ,  G01N30/88 ,  G01N33/94

CPC分类号： G01N33/9406 ,  G01N30/88 ,  Y10T436/142222 ,  Y10T436/173845 ,  Y10T436/203332 ,  Y10T436/255

摘要： Catecholamines can be detected with high sensitivity in a shortened time period, by introducing a sample containing catecholamines into an adsorption column packed with an adsorbent to adsorb catecholamines to the column, then introducing a reagent for derivatization to effect pre-column labeling and then analyzing the pre-column labeled catecholamines by means of high performance liquid chromatography. Connection of the adsorption column and a separation column of high performance liquid chromatography via a fluid path changeover valve enables to automated analysis of catecholamines.

摘要翻译： 可以在缩短的时间内以高灵敏度检测儿茶酚胺，通过将含有儿茶酚胺的样品引入填充有吸附剂的吸附塔中将儿茶酚胺吸附到塔中，然后引入用于衍生化的试剂进行预柱标记，然后分析 通过高效液相色谱法预柱标记的儿茶酚胺。 通过流体路径切换阀连接吸附塔和高效液相色谱分离柱，可以自动分析儿茶酚胺。

公开(公告)号：US5005434A

公开(公告)日：1991-04-09

申请号：US390720

申请日：1989-08-08

申请人： Yoshio Watanabe ,  Mamoru Taki ,  Junkichi Miura ,  Masao Kamahori ,  Hiroyuki Miyagi ,  Yasuhide Matsumura ,  Masafumi Kanetomo

发明人： Yoshio Watanabe ,  Mamoru Taki ,  Junkichi Miura ,  Masao Kamahori ,  Hiroyuki Miyagi ,  Yasuhide Matsumura ,  Masafumi Kanetomo

IPC分类号： G01N35/10 ,  G01N1/00 ,  G01N1/14 ,  G01N15/00 ,  G01N30/16 ,  G01N30/24 ,  G01N30/34

CPC分类号： G01N35/1097 ,  G01N1/14 ,  G01N2015/0011 ,  G01N2021/4735 ,  G01N2201/08 ,  G01N30/34

摘要： An autosampler consists of a needle to draw specimen from a specimen container into a passage therein; a block having a passage that follows the passge in the needle; a suction mechanism for drawing in the specimen by suction through the passages in the needle and the block; a discharge mechanism for discarding the specimen drawn in by the suction mechanism; and a detecting device for detecting air bubble in the specimen, the detecting device being embedded in the block in such a manner as to face the passage in the block. The detecting device detects the air bubbles in the specimen according to changes in physical or chemical properties of the specimen passing through the passage in the block. The discharge mechanism is activated by the detecting device to discharge air-mixed specimen. When even a small amount of air is drawn into the passage from the specimen container, in which only a small amount of specimen is contained, the air specimen can reliably be distinguished from normal specimen by this autosampler. Therefore the reliability of the system using the autosampler can be significantly improved.

摘要翻译： 自动进样器由将样本从样本容器拉入其中的通道的针头组成; 具有跟随针头中的传递的通道的块; 抽吸机构，用于通过抽吸通过针和块中的通道抽吸样品; 用于废弃由抽吸机构吸入的试样的排出机构; 以及用于检测样本中的气泡的检测装置，所述检测装置以面对块中的通道的方式嵌入块中。 检测装置根据通过块体中的通道的试样的物理或化学特性的变化来检测样品中的气泡。 排出机构由检测装置启动，以排出空气混合样品。 即使少量的空气从容纳少量试样的试样容器的通道中吸入，也可以通过该自动进样器将空气样本与正常试样区分开。 因此，可以显着提高使用自动进样器的系统的可靠性。