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公开(公告)号：US20170029865A1
公开(公告)日：2017-02-02
申请号：US15296254
申请日：2016-10-18
申请人： Kazuma Shiota , Hirofumi Horiguchi , Ai Iyotani , Jun Yoshikawa , Tomoko Sato
发明人： Kazuma Shiota , Hirofumi Horiguchi , Ai Iyotani , Jun Yoshikawa , Tomoko Sato
CPC分类号： C12Q1/34 , A23C9/1206 , C12N9/16 , C12N9/2402 , C12N9/2468 , C12N9/2471 , C12Q1/44 , C12Y301/06001 , C12Y302/01023 , C12Y302/01108 , G01N2333/916
摘要： A lactase preparation includes lactase and has a lactase activity of 4,000 NLU/g or more according to the FCC IV method, wherein the lactase originates from a lactase gene of yeast, and wherein the lactase preparation has an arylsulfatase activity of 0.1% or less based on the lactase activity, in which the arylsulfatase activity (unit: U/g) is determined and calculated.
摘要翻译： 乳糖酶制剂包括乳糖酶，并且根据FCC IV方法具有4000NUU / g以上的乳糖酶活性，其中所述乳糖酶源自酵母的乳糖酶基因，并且其中所述乳糖酶制剂的芳基硫酸酯酶活性为0.1％以下 关于乳糖酶活性，其中测定和计算芳基硫酸酯酶活性（单位：U / g）。
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公开(公告)号：US20140087404A1
公开(公告)日：2014-03-27
申请号：US14004687
申请日：2012-03-13
申请人： Kazuma Shiota , Hirofumi Horiguchi , Ai Iyotani , Jun Yoshikawa , Tomoko Sato
发明人： Kazuma Shiota , Hirofumi Horiguchi , Ai Iyotani , Jun Yoshikawa , Tomoko Sato
CPC分类号： C12Q1/34 , A23C9/1206 , C12N9/16 , C12N9/2402 , C12N9/2468 , C12N9/2471 , C12Q1/44 , C12Y301/06001 , C12Y302/01023 , C12Y302/01108 , G01N2333/916
摘要： Provided is a method for determining activity of arylsulfatase in an aqueous system, which comprises a step in which arylsulfatase is subjected to reaction with a substrate, from which fluorophore or chromophore is liberated by suffering an action of the arylsulfatase, in an aqueous reaction system having high ionic strength. Also, provided are a lactase preparation having a lactase activity of 4,000 NLU/g or more according to the FCC IV method and having an arylsulfatase activity of 0.1% or less of the lactase activity as the basis, in which the arylsulfatase activity has been determined by the method for determining activity of arylsulfatase in an aqueous system according to the fluorescence method of the present invention; a method for producing this preparation; and a dairy product which comprises using this preparation.
摘要翻译： 提供了一种用于测定水性体系中芳基硫酸酯酶的活性的方法，其包括其中芳基硫酸酯酶与基质反应的步骤，其中通过在芳基硫酸酯酶的作用下从其中释放荧光团或发色团， 高离子强度。 此外，根据FCC IV方法，提供乳糖酶活性为4000NUU / g以上的乳糖酶制剂，其乳糖酶活性的芳基硫酸酯酶活性为0.1％以下，其中确定了芳基硫酸酯酶活性 通过根据本发明的荧光法测定水性体系中芳基硫酸酯酶的活性的方法; 制备该制剂的方法; 以及包括使用该制剂的乳制品。
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公开(公告)号：US20120199480A1
公开(公告)日：2012-08-09
申请号：US13503040
申请日：2010-11-17
申请人： Kazuma Shiota , Tomohiro Fujita
发明人： Kazuma Shiota , Tomohiro Fujita
IPC分类号： G01N27/447 , G01N33/50 , C12N9/52
CPC分类号： C12N9/52 , C07K1/26 , G01N27/447
摘要： Provided is a means for accurately analyzing a protease by electrophoresis.Disclosed is an electrophoretic analysis method for analyzing a protease-containing sample, is characterized by exposing a sample containing a protease to be analyzed, to pH conditions under which the protease is rapidly deactivated, and then subjecting the sample to electrophoresis.
摘要翻译： 提供了通过电泳准确分析蛋白酶的方法。 公开了用于分析含蛋白酶的样品的电泳分析方法，其特征在于将含有待分析蛋白酶的样品暴露于蛋白酶快速失活的pH条件下，然后对样品进行电泳。
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公开(公告)号：US08951397B2
公开(公告)日：2015-02-10
申请号：US13503040
申请日：2010-11-17
申请人： Kazuma Shiota , Tomohiro Fujita
发明人： Kazuma Shiota , Tomohiro Fujita
IPC分类号： G01N33/50 , C12N9/52 , C07K1/26 , G01N27/447
CPC分类号： C12N9/52 , C07K1/26 , G01N27/447
摘要： Provided is a means for accurately analyzing a protease by electrophoresis.Disclosed is an electrophoretic analysis method for analyzing a protease-containing sample, is characterized by exposing a sample containing a protease to be analyzed, to pH conditions under which the protease is rapidly deactivated, and then subjecting the sample to electrophoresis.
摘要翻译： 提供了通过电泳准确分析蛋白酶的方法。 公开了用于分析含蛋白酶的样品的电泳分析方法，其特征在于将含有待分析蛋白酶的样品暴露于蛋白酶快速失活的pH条件下，然后对样品进行电泳。
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