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公开(公告)号：US6139838A
公开(公告)日：2000-10-31
申请号：US923999
申请日：1997-09-05
CPC分类号： A61K38/49
摘要： The present invention provides a t-PA medicinal preparation obtained by remarkably improving the solubility and stability of t-PA or modified t-PA. Nicotinamide or a derivative thereof is incorporated into t-PA or modified t-PA-containing solvent system. If necessary, a citrate buffer solution is also used. The t-PA-containing medicinal composition is in the form of, for example, a freeze-dried preparation.
摘要翻译： 本发明提供了通过显着提高t-PA或改性t-PA的溶解度和稳定性得到的t-PA药物制剂。 烟酰胺或其衍生物掺入t-PA或改性的含t-PA的溶剂体系中。 如果需要，还使用柠檬酸盐缓冲溶液。 含t-PA的药物组合物是例如冷冻干燥制剂的形式。
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公开(公告)号：US07001885B2
公开(公告)日：2006-02-21
申请号：US10175382
申请日：2002-06-20
申请人： Satoshi Adachi , Hiroshi Mizokami , Yoshitaka Tajima , Yoshinobu Miyatsu , Toshinobu Nouchi , Yoshitaka Ushio
发明人： Satoshi Adachi , Hiroshi Mizokami , Yoshitaka Tajima , Yoshinobu Miyatsu , Toshinobu Nouchi , Yoshitaka Ushio
IPC分类号： A61K38/00
CPC分类号： C07K14/765 , C07K1/18
摘要： Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.
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公开(公告)号：US06908749B2
公开(公告)日：2005-06-21
申请号：US10175103
申请日：2002-06-20
申请人： Toshinobu Nouchi , Hiroshi Mizokami , Yoshitaka Tajima , Yoshinobu Miyatsu , Masahiro Sakaguchi , Kazunari Yagi
发明人： Toshinobu Nouchi , Hiroshi Mizokami , Yoshitaka Tajima , Yoshinobu Miyatsu , Masahiro Sakaguchi , Kazunari Yagi
IPC分类号： C07K1/30 , C07K14/765 , C12P21/06
CPC分类号： C07K14/765
摘要： A method for preparing human serum albumin comprises the step of heat-treating a human serum albumin solution containing contaminants at a pH value in the proximity to the isoelectric point of the contaminants.
摘要翻译： 制备人血清白蛋白的方法包括在接近污染物的等电点的pH值下热处理含有污染物的人血清白蛋白溶液的步骤。
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4.发明申请公开(公告)号：US20060258850A1
公开(公告)日：2006-11-16
申请号：US11489541
申请日：2006-07-20
IPC分类号： C12P21/06 , C07H21/04 , C07K14/765
CPC分类号： C07K1/30 , C07K14/765
摘要： The present invention herein discloses a method for purifying human serum albumin, which is characterized in that it comprises the steps of heat-treating a human serum albumin-containing solution including impurities originated from a host cell, in the presence of a divalent cation such as calcium ion, magnesium ion, nickel ion, cobalt ion, iron ion and zinc ion to thus selectively allow the impurities to undergo agglutination. The present invention also provides the highly purified human serum albumin prepared by the foregoing method.
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5.发明授权Method for preparing human serum albumin through heat-treatment in the presence of divalent cation 有权在二价阳离子存在下通过热处理制备人血清白蛋白的方法公开(公告)号：US07531631B2
公开(公告)日：2009-05-12
申请号：US11489541
申请日：2006-07-20
IPC分类号： C07K14/00
CPC分类号： C07K1/30 , C07K14/765
摘要： The present invention herein discloses a method for purifying human serum albumin, which is characterized in that it comprises the steps of heat-treating a human serum albumin-containing solution including impurities originated from a host cell, in the presence of a divalent cation such as calcium ion, magnesium ion, nickel ion, cobalt ion, iron ion and zinc ion to thus selectively allow the impurities to undergo agglutination. The present invention also provides the highly purified human serum albumin prepared by the foregoing method.
摘要翻译： 本发明的本发明公开了一种纯化人血清白蛋白的方法，其特征在于，其包括在二价阳离子如二氯甲烷存在下热处理含有源自宿主细胞的杂质的人血清白蛋白溶液的步骤 钙离子，镁离子，镍离子，钴离子，铁离子和锌离子，从而选择性地允许杂质发生凝集。 本发明还提供通过上述方法制备的高纯度人血清白蛋白。
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公开(公告)号：US06869934B2
公开(公告)日：2005-03-22
申请号：US10088588
申请日：2001-07-18
申请人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
发明人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
CPC分类号： C12N9/6432 , C07K14/4721 , C07K14/4728 , C12Y304/21006
摘要： The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
摘要翻译： 本发明涉及通过阳离子交换层析纯化钙离子结合蛋白的方法。 本发明提供了从含有钙离子结合蛋白质和污染物的液体样品中以简单有效的方式分离和纯化钙离子结合蛋白的方法，而没有任何预处理如加入螯合剂。 更具体地，本发明涉及一种纯化钙离子结合蛋白的方法，其包括在钙离子存在下使所述蛋白质与阳离子交换载体接触以使所述蛋白质吸附到载体上，并且在洗涤后，洗脱 所述蛋白质和基本上不含有通过本发明的方法获得的污染物的钙离子结合蛋白质。
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公开(公告)号：US20050059805A1
公开(公告)日：2005-03-17
申请号：US10944810
申请日：2004-09-21
申请人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
发明人： Hiroshi Mizokami , Shinichi Furukawa , Keishin Sugawara , Tatsufumi Onchi , Kazuhiro Komatsu , Satoshi Koyanagi , Hideo Yoshizaki
CPC分类号： C12N9/6432 , C07K14/4721 , C07K14/4728 , C12Y304/21006
摘要： The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
摘要翻译： 本发明涉及通过阳离子交换层析纯化钙离子结合蛋白的方法。 本发明提供了从含有钙离子结合蛋白质和污染物的液体样品中以简单有效的方式分离和纯化钙离子结合蛋白的方法，而没有任何预处理如加入螯合剂。 更具体地，本发明涉及一种纯化钙离子结合蛋白的方法，其包括在钙离子存在下使所述蛋白质与阳离子交换载体接触以使所述蛋白质吸附到载体上，并且在洗涤后，洗脱 所述蛋白质和基本上不含有通过本发明的方法获得的污染物的钙离子结合蛋白质。
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公开(公告)号：US07667009B2
公开(公告)日：2010-02-23
申请号：US10555883
申请日：2004-04-14
申请人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
发明人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
IPC分类号： A23J1/00
CPC分类号： C07K14/43531 , A61K38/00 , A61K39/00
摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.
摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改性主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的修饰的主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。
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公开(公告)号：US20080113424A1
公开(公告)日：2008-05-15
申请号：US10555883
申请日：2004-04-14
申请人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
发明人： Satoshi Koyanagi , Kenjiro Kawatsu , Toshio Murakami , Yoshinobu Miyatsu , Toshihiro Maeda , Hiroshi Mizokami
CPC分类号： C07K14/43531 , A61K38/00 , A61K39/00
摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.
摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改良的主要螨变应原。 一种用于纯化通过遗传重组技术获得的改良的主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的改性主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量成分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。
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公开(公告)号：US4563303A
公开(公告)日：1986-01-07
申请号：US722381
申请日：1985-04-12
申请人： Akihiro Ginnaga , Shin Sakuma , Tsukasa Nishihara , Tomitaka Tashiro , Sadao Susumi , Tetsuo Kawahara , Hiroshi Mizokami
发明人： Akihiro Ginnaga , Shin Sakuma , Tsukasa Nishihara , Tomitaka Tashiro , Sadao Susumi , Tetsuo Kawahara , Hiroshi Mizokami
IPC分类号： C12P21/00 , A61K39/00 , C07K14/235 , A61K39/10
CPC分类号： C07K14/235 , A61K39/00 , Y10S435/822 , Y10S530/825
摘要： Improved method for the purification of filamentous hemagglutinin (F-HA) on industrial scale which comprises contacting a culture of a microorganism of the genus Bordetella with a cellulose sulfate gel, a polysaccharide gel chemically bound with dextran sulfate, or a crosslinked polysaccharide sulfate gel, thereby adsorbing F-HA on the gel, and then eluting F-HA from the gel. Said method can give a highly purified F-HA which does not contain any other proteins, lipid, saccharides, etc. and further undesirable endotoxin, and hence can be used for producing various reagents, medicines and pertussis vaccine.
摘要翻译： 用于工业规模净化丝状血凝素（F-HA）的改进方法，其包括将博德特氏菌属的微生物的培养物与硫酸纤维素凝胶接触，与硫酸葡聚糖化学结合的多糖凝胶或交联的多糖硫酸凝胶， 从而在凝胶上吸附F-HA，然后从凝胶中洗脱F-HA。 所述方法可以得到不含任何其它蛋白质，脂质，糖类等的高度纯化的F-HA，以及不希望的内毒素，因此可用于生产各种试剂，药物和百日咳疫苗。
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