公开(公告)号：US08722408B2

公开(公告)日：2014-05-13

申请号：US12987601

申请日：2011-01-10

申请人： Ken-ichi Yamamura ,  Kimi Araki

发明人： Ken-ichi Yamamura ,  Kimi Araki

IPC分类号： C12N15/85 ,  C12N15/63

CPC分类号： C12N15/1051 ,  C12N15/10

摘要： A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.

摘要翻译： 含有由反向重复序列1，间隔序列和反向重复序列2构成的loxP序列的陷阱载体，loxP序列是突变型loxP，其中反向重复序列1或2的一部分突变。

公开(公告)号：US07312075B1

公开(公告)日：2007-12-25

申请号：US10030658

申请日：2000-05-02

申请人： Ken-ichi Yamamura ,  Kimi Araki

发明人： Ken-ichi Yamamura ,  Kimi Araki

IPC分类号： C12N15/63

CPC分类号： C12N15/1051 ,  C12N15/10

摘要： A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.

摘要翻译： 含有由反向重复序列1，间隔序列和反向重复序列2构成的loxP序列的陷阱载体，loxP序列是突变型loxP，其中反向重复序列1或2的一部分突变。

公开(公告)号：US20150052625A1

公开(公告)日：2015-02-19

申请号：US14387704

申请日：2012-03-27

申请人： Ken-ichi Yamamura ,  Kimi Araki ,  Seiji Okada ,  Akihiko Shimono

发明人： Ken-ichi Yamamura ,  Kimi Araki ,  Seiji Okada ,  Akihiko Shimono

IPC分类号： A01K67/027 ,  C12N5/0735

CPC分类号： A01K67/0278 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/20 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0331 ,  A01K2267/035 ,  C12N5/0606 ,  C12N2517/02

摘要： The present invention provides embryonic stem cells obtainable from an embryo of an immunodeficient mouse which is deficient in both Rag2 and Jak3 genes by culture in the presence of a GSK3 inhibitor and an MEK inhibitor, as well as a transgenic mouse, which is created with the use of these embryonic stem cells.

摘要翻译： 本发明提供了通过在GSK3抑制剂和MEK抑制剂的存在下培养而Rag2和Jak3基因缺陷的免疫缺陷小鼠的胚胎获得的胚胎干细胞，以及转基因小鼠，其由 使用这些胚胎干细胞。

公开(公告)号：US20090202988A1

公开(公告)日：2009-08-13

申请号：US11907497

申请日：2007-10-12

申请人： Ken-ichi Yamamura ,  Kimi Araki

发明人： Ken-ichi Yamamura ,  Kimi Araki

IPC分类号： C12Q1/68 ,  C12N15/00 ,  C12N5/06

CPC分类号： C12N15/1051 ,  C12N15/10

摘要： A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.

摘要翻译： 含有由反向重复序列1，间隔序列和反向重复序列2构成的loxP序列的陷阱载体，loxP序列是突变型loxP，其中反向重复序列1或2的一部分突变。

公开(公告)号：US5770400A

公开(公告)日：1998-06-23

申请号：US814468

申请日：1997-03-10

申请人： Junichi Miyazaki ,  Ken-ichi Yamamura ,  Masatake Araki ,  Hiroshi Yonemura ,  Chikateru Nozaki

发明人： Junichi Miyazaki ,  Ken-ichi Yamamura ,  Masatake Araki ,  Hiroshi Yonemura ,  Chikateru Nozaki

IPC分类号： C07K14/02 ,  C12N9/38 ,  C12N15/85 ,  C12N15/70 ,  C12P21/02

CPC分类号： C07K14/005 ,  C12N15/85 ,  C12N9/2471 ,  C12Y302/01023 ,  C12N2730/10122 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/005 ,  C12N2830/15 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick .beta.-actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.

公开(公告)号：US5811260A

公开(公告)日：1998-09-22

申请号：US373143

申请日：1989-06-23

申请人： Junichi Miyazaki ,  Ken-ichi Yamamura ,  Masatake Araki ,  Hiroshi Yonemura ,  Chikateru Nozaki

发明人： Junichi Miyazaki ,  Ken-ichi Yamamura ,  Masatake Araki ,  Hiroshi Yonemura ,  Chikateru Nozaki

IPC分类号： C07K14/02 ,  C12N9/38 ,  C12N15/85 ,  C12P21/02

CPC分类号： C07K14/005 ,  C12N15/85 ,  C12N9/2471 ,  C12Y302/01023 ,  C12N2730/10122 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/005 ,  C12N2830/15 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick .beta.-actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.

摘要翻译： 用于在动物细胞中表达外源基因的表达载体，其含有小鸡β-肌动蛋白基因启动子和用于在所述启动子的下游并入外源基因的限制酶位点和表达外源基因的方法，其中 包括将所述外源基因并入限制酶位点的表达载体中，用于掺入外源基因，将所述载体导入动物细胞并培养获得的转化的动物细胞。 本发明的表达系统可以应用于任何外源基因的表达，具有极高的表达效率，适用于广泛的宿主细胞，因此可充分利用于有用材料的工业规模生产 。