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    Cellulase variants
    1.
    发明申请
    Cellulase variants 审中-公开
    纤维素酶变体

    公开(公告)号：US20050009166A1

    公开(公告)日：2005-01-13

    申请号：US10919195

    申请日：2004-08-16

    申请人： Kim Andersen ,  Martin Schulein ,  Hanne Dela ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Andersen ,  Martin Schulein ,  Hanne Dela ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/15 ,  C12N1/21 ,  C12N9/42 ,  C12R1/645 ,  C12S11/00 ,  C12S99/00 ,  D06L4/40 ,  D06M16/00 ,  D21B1/02 ,  D21C5/02 ,  D21H21/24 ,  C07H21/04 ,  C12N15/74

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase variants
    2.
    发明授权
    Cellulase variants 有权
    纤维素酶变体

    公开(公告)号：US08017372B2

    公开(公告)日：2011-09-13

    申请号：US11830063

    申请日：2007-07-30

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen, legal representative ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42 ,  C12N15/00 ,  C12P21/04 ,  C07H21/04

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase Variants
    3.
    发明申请
    Cellulase Variants 有权
    纤维素酶变体

    公开(公告)号：US20080206836A1

    公开(公告)日：2008-08-28

    申请号：US11830063

    申请日：2007-07-30

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    CELLULASE VARIANTS
    5.
    发明申请
    CELLULASE VARIANTS 审中-公开
    纤维素酶变体

    公开(公告)号：US20110250674A1

    公开(公告)日：2011-10-13

    申请号：US13162636

    申请日：2011-06-17

    申请人： Kim Vilbour Andersen ,  Martin Schülein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schülein ,  Torben Henriksen ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。

    Cellulase Variants
    6.
    发明申请
    Cellulase Variants 审中-公开
    纤维素酶变体

    公开(公告)号：US20120289450A1

    公开(公告)日：2012-11-15

    申请号：US13471757

    申请日：2012-05-15

    申请人： Kim Vilbour Andersen ,  Martin Schülein ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schülein ,  Lars Christiansen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C12N9/42 ,  C11D3/386

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to cellulase variants, i.e., endo-beta-1,4-glucanase variants, derived from a parental cellulase, i.e., endo-beta-1,4-glucanase, by substitution, insertion and/or deletion, which variant has a catalytic core domain, in which the variant at position 5 holds an alanine residue (A), a serine residue (S), or a threonine residue (T); at position 8 holds a phenylalanine residue (F), or a tyrosine residue (Y); at position 9 holds a phenylalanine residue (F), a tryptophan residue (W), or a tyrosine residue (Y); at position 10 holds an aspartic acid residue (D); and at position 121 holds an aspartic acid residue (D).

    摘要翻译： 本发明涉及通过取代，插入和/或缺失衍生自亲代纤维素酶（即内切-β-1,4-葡聚糖酶）的纤维素酶变体，即内切-β-1,4-葡聚糖酶变体，该变体 具有催化核心结构域，其中第5位的变体含有丙氨酸残基（A），丝氨酸残基（S）或苏氨酸残基（T）; 在8位保持苯丙氨酸残基（F）或酪氨酸残基（Y）; 位置9含有苯丙氨酸残基（F），色氨酸残基（W）或酪氨酸残基（Y）; 在10位保持天冬氨酸残基（D）; 并且在位置121处保持天冬氨酸残基（D）。

    CELLULASE VARIANTS
    7.
    发明申请
    CELLULASE VARIANTS 有权
    纤维素酶变体

    公开(公告)号：US20090170747A1

    公开(公告)日：2009-07-02

    申请号：US12394202

    申请日：2009-02-27

    申请人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christensen ,  Bo Damgaard ,  Claus Von der Osten

    发明人： Kim Vilbour Andersen ,  Martin Schulein ,  Torben Henriksen ,  Lars Christensen ,  Bo Damgaard ,  Claus Von der Osten

    IPC分类号： C11D3/386 ,  C12N9/42

    CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

    摘要： The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.

    摘要翻译： 本发明涉及通过氨基酸取代，缺失或插入改进纤维素分解酶的性质的方法，该方法包括以下步骤：a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V（EGV）的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代，缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代，缺失或插入。 还描述了通过该方法获得的纤维素酶变体。