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公开(公告)号:US20050009166A1
公开(公告)日:2005-01-13
申请号:US10919195
申请日:2004-08-16
申请人: Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人: Kim Andersen , Martin Schulein , Hanne Dela , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号: C12N15/09 , C11D3/386 , C12N1/15 , C12N1/21 , C12N9/42 , C12R1/645 , C12S11/00 , C12S99/00 , D06L4/40 , D06M16/00 , D21B1/02 , D21C5/02 , D21H21/24 , C07H21/04 , C12N15/74
CPC分类号: C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译: 本发明涉及通过氨基酸取代,缺失或插入改进纤维素分解酶的性质的方法,该方法包括以下步骤:a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V(EGV)的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代,缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代,缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号:US08017372B2
公开(公告)日:2011-09-13
申请号:US11830063
申请日:2007-07-30
申请人: Kim Vilbour Andersen , Martin Schulein , Torben Henriksen, legal representative , Lars Christiansen , Bo Damgaard , Claus Von der Osten
CPC分类号: C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译: 本发明涉及通过氨基酸取代,缺失或插入改进纤维素分解酶的性质的方法,该方法包括以下步骤:a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V(EGV)的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代,缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代,缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号:US20080206836A1
公开(公告)日:2008-08-28
申请号:US11830063
申请日:2007-07-30
申请人: Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
发明人: Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christiansen , Bo Damgaard , Claus Von der Osten
IPC分类号: C12N9/42
CPC分类号: C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译: 本发明涉及通过氨基酸取代,缺失或插入改进纤维素分解酶的性质的方法,该方法包括以下步骤:a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V(EGV)的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代,缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代,缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号:US20090170747A1
公开(公告)日:2009-07-02
申请号:US12394202
申请日:2009-02-27
申请人: Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christensen , Bo Damgaard , Claus Von der Osten
发明人: Kim Vilbour Andersen , Martin Schulein , Torben Henriksen , Lars Christensen , Bo Damgaard , Claus Von der Osten
CPC分类号: C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of:a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
摘要翻译: 本发明涉及通过氨基酸取代,缺失或插入改进纤维素分解酶的性质的方法,该方法包括以下步骤:a。 构建已知具有类似于蛋白质数据库条目4ENG中已知的Humicola insolens的内切葡聚糖酶V(EGV)的三维结构的至少两个氨基酸序列的多重比对; b。 基于EGV的结构构建纤维素分解酶的同源构建的三维结构; C。 鉴定存在于与所述基质结合裂缝一定距离的氨基酸残基位置不大于5埃; d。 鉴定酶的表面暴露的氨基酸残基; e。 鉴定酶的所有带电荷或潜在带电的氨基酸残基位置; F。 选择其中氨基酸残基被取代,缺失或提供插入的一个或多个位置; 和g。 通过使用常规蛋白质工程技术进行取代,缺失或插入。 还描述了通过该方法获得的纤维素酶变体。
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公开(公告)号:US07993898B2
公开(公告)日:2011-08-09
申请号:US12394202
申请日:2009-02-27
申请人: Kim Vilbour Andersen , Martin Schulein , Torben Henriksen, legal representative , Lars Christensen , Bo Damgaard , Claus Von der Osten
CPC分类号: C11D3/38645 , C12N9/2437 , C12Y302/01004
摘要: The present invention relates to a method for improving the properties of a cellulolytic enzyme by amino acid substitution, deletion or insertion, the method comprising the steps of: a. constructing a multiple alignment of at least two amino acid sequences known to have three-dimensional structures similar to endoglucanase V (EGV) from Humicola insolens known from Protein Data Bank entry 4ENG; b. constructing a homology-built three-dimensional structure of the cellulolytic enzyme based on the structure of the EGV; c. identifying amino acid residue positions present in a distance from the substrate binding cleft of not more than 5 Å; d. identifying surface-exposed amino acid residues of the enzyme; e. identifying all charged or potentially charged amino acid residue positions of the enzyme; f. choosing one or more positions wherein the amino acid residue is to be substituted, deleted or where an insertion is to be provided; and g. carrying out the substitution, deletion or insertion by using conventional protein engineering techniques. Also described are cellulase variants obtained by this method.
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公开(公告)号:US6117664A
公开(公告)日:2000-09-12
申请号:US709974
申请日:1996-09-09
IPC分类号: C11D3/386 , C11D11/00 , C12N9/42 , C12S9/00 , C12S11/00 , D06M16/00 , D06P5/02 , D06P5/13 , D06P5/15 , D21C5/00 , A61K38/47
CPC分类号: D21C5/005 , C11D3/386 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/137 , D06P5/158
摘要: The present invention relates to variants of a Family 7 cellulase comprising one or more mutations of the amino acid sequence of the cellulase in which each mutation is independently a substitution of an amino acid residue with another amino acid or an insertion of at least one amino acid residues, wherein the variant has a modified H-bonding potential and hydrophobic interaction relative to the cellulase. The present invention also relates to the use of the cellulase variants in detergent compositions, especially for soil removal or color clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for deinking of old paper; for plant degradation.
摘要翻译: 本发明涉及家族7纤维素酶的变体,其包含纤维素酶的氨基酸序列的一个或多个突变,其中每个突变独立地是氨基酸残基与另一个氨基酸的取代或至少一个氨基酸的插入 残基,其中变体具有相对于纤维素酶的修饰的H键合电位和疏水相互作用。 本发明还涉及纤维素酶变体在洗涤剂组合物中的用途,特别是用于去除污垢或着色澄清或防止背染; 织物柔软剂; 用于纺织品的生物抛光; 用于纸浆排水改良; 用于脱墨的旧纸; 用于植物降解。
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公开(公告)号:US5912157A
公开(公告)日:1999-06-15
申请号:US709979
申请日:1996-09-09
IPC分类号: C11D3/386 , C11D11/00 , C12N9/42 , C12S9/00 , C12S11/00 , D06M16/00 , D06P5/02 , D06P5/13 , D06P5/15 , D21C5/00
CPC分类号: D21C5/005 , C11D3/386 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/137 , D06P5/158
摘要: The present invention relates to cellulases derived from the strain Myceliophthora thermophila and variants thereof comprising a core and optionally a C-terminal link consisting of 10 amino acids at the most, especially cellulases having a substrate binding cleft of a depth of at least 12 .ANG., exhibit enhanced enzyme activity in the alkaline pH range while exerting a moderate cellulolytic action on the cellulosic substrate and are, for example, useful in detergent compositions, especially for soil removal or color clarification or preventing backstaining; in fabric softeners; for bio-polishing of textiles; for drainage improvement of paper pulp; for deinking of old paper; for plant degradation.
摘要翻译: 本发明涉及源自嗜热丝菌嗜热链霉菌菌株的纤维素酶及其变体,其包含核心和任选的由最多10个氨基酸组成的C-末端连接体,特别是具有至少12个神经元深度的底物结合裂缝的纤维素酶, 在碱性pH范围内表现出增强的酶活性,同时在纤维素基质上施加中等的纤维素分解作用,并且例如可用于洗涤剂组合物,特别是用于去除污垢或着色澄清或防止背染; 织物柔软剂; 用于纺织品的生物抛光; 用于纸浆排水改良; 用于脱墨的旧纸; 用于植物降解。
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公开(公告)号:US08071351B2
公开(公告)日:2011-12-06
申请号:US12779112
申请日:2010-05-13
CPC分类号: C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要: The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译: 本发明涉及属于糖基水解酶家族44的木葡聚糖酶,并且具有pH5至pH8之间至少30%的相对木葡聚糖酶活性,来源于Paenibacillus属,特别是来自多粘杆菌(Paenibacillus polymyxa)或Paenibacillus sp。 木葡聚糖酶在常规洗涤剂组合物中表现出高性能。
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公开(公告)号:US20080138880A1
公开(公告)日:2008-06-12
申请号:US11970559
申请日:2008-01-08
申请人: Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
发明人: Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
CPC分类号: C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要: The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译: 本发明涉及属于糖基水解酶家族44的木葡聚糖酶,并且具有pH5至pH8之间至少30%的相对木葡聚糖酶活性,来源于Paenibacillus属,特别是来自多粘杆菌(Paenibacillus polymyxa)或Paenibacillus sp。 木葡聚糖酶在常规洗涤剂组合物中表现出高性能。
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公开(公告)号:US20120079665A1
公开(公告)日:2012-04-05
申请号:US13287166
申请日:2011-11-02
申请人: Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
发明人: Kirk Schnorr , Per Lina Jorgensen , Martin Schulein , Hanne Dela
CPC分类号: C12N9/24 , C11D3/38636 , C11D3/38645 , C12N9/2405 , C12N9/248 , C12Y302/01151 , D06L4/40 , D06M16/003
摘要: The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
摘要翻译: 本发明涉及属于糖基水解酶家族44的木葡聚糖酶,并且具有pH5至pH8之间至少30%的相对木葡聚糖酶活性,来源于Paenibacillus属,特别是来自多粘杆菌(Paenibacillus polymyxa)或Paenibacillus sp。 木葡聚糖酶在常规洗涤剂组合物中表现出高的性能。
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