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11 条记录 (耗时 0.025 秒)

    Extracellular expression of cellulose binding domains (CBD) using Bacillus
    6.
    发明授权
    Extracellular expression of cellulose binding domains (CBD) using Bacillus 失效
    使用芽孢杆菌的纤维素结合结构域（CBD）的细胞外表达

    公开(公告)号：US6060274A

    公开(公告)日：2000-05-09

    申请号：US959212

    申请日：1997-10-28

    申请人： Mads Eskelund Bjornvad ,  Martin Schulein ,  Per Lina Jorgensen

    发明人： Mads Eskelund Bjornvad ,  Martin Schulein ,  Per Lina Jorgensen

    IPC分类号： C12N15/09 ,  C07K14/195 ,  C07K14/21 ,  C07K14/33 ,  C12N1/21 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/07 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C07K14/195 ,  C07K14/21 ,  C07K14/33

    摘要： The present invention relates to Bacillus hosts transformed with a vector comprising a DNA sequence encoding for a cellulose binding domain (CBD) and capable of expressing said sequence, the expressed polypeptide protein consisting essentially of one or more non-catalytic domains; the cellulose binding domain having a molecular weight in the range of from 4 kD to 35 kD and being obtainable from a microorganism or from a plant, preferably from a bacterium or a fungus; the Bacillus host e.g. being one of the species Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Bacillus stearothermophilos, and Bacillus amyloliquefaciens; and a Bacillus expression vector carrying an inserted DNA sequence encoding for a cellulose binding domain; and a method for producing a cellulose binding domain polypeptide in a Bacillus host cell.

    摘要翻译： 本发明涉及用包含编码纤维素结合结构域（CBD）并能够表达所述序列的DNA序列的载体转化的芽孢杆菌宿主，所述表达的多肽蛋白质基本上由一个或多个非催化结构域组成; 所述纤维素结合结构域具有4kD至35kD的分子量，可从微生物或植物，优选来自细菌或真菌得到; 芽孢杆菌宿主例如 是枯草芽孢杆菌，地衣芽孢杆菌，巨大芽孢杆菌，嗜热脂肪芽孢杆菌和解淀粉芽孢杆菌之一; 和携带编码纤维素结合结构域的插入DNA序列的芽孢杆菌表达载体; 以及在芽孢杆菌宿主细胞中生产纤维素结合结构域多肽的方法。

    Xyloglucan-specific alkaline xyloglucanase from bacillus
    7.
    发明授权
    Xyloglucan-specific alkaline xyloglucanase from bacillus 失效
    来自芽孢杆菌的葡聚糖特异性碱性木葡聚糖酶

    公开(公告)号：US06268197B1

    公开(公告)日：2001-07-31

    申请号：US09110959

    申请日：1998-07-07

    申请人： Martin Schulein ,  Helle Outtrup ,  Per Lina Jorgensen ,  Mads Eskelund Bjornvad

    发明人： Martin Schulein ,  Helle Outtrup ,  Per Lina Jorgensen ,  Mads Eskelund Bjornvad

    IPC分类号： C12N942

    CPC分类号： C12N9/2434 ,  C11D3/38636

    摘要： A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.

    摘要翻译： 在pH7下具有至少50％的相对木葡聚糖酶活性和无或不显着的纤维素分解活性的木葡聚糖酶可以例如获得。 来自芽孢杆菌菌株。 包含SEQ ID NO：2的位置30-261所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如地衣芽孢杆菌ATCC 14580，并且可以由包含如SEQ ID NO所示的核苷酸序列的多核苷酸分子编码 ：1个从核苷酸88到核苷酸783; 并且包含SEQ ID NO：4的位置1-537所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如琼脂糖琼脂糖凝胶，NCIMB 40482，并且可以由包含SEQ ID NO：4所示的核苷酸序列的多核苷酸分子编码 SEQ ID NO：3从核苷酸1到核苷酸1611.木葡聚糖酶是有用的，例如 在清洁组合物和纤维素纤维的处理中。