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    Transglutaminase originating from Crassostrea gigas
    1.
    发明授权
    Transglutaminase originating from Crassostrea gigas 失效
    源自Crassostrea gigas的转谷氨酰胺酶

    公开(公告)号：US5736356A

    公开(公告)日：1998-04-07

    申请号：US525654

    申请日：1995-09-28

    申请人： Kohichiro Sano ,  Yoshiyuki Kumazawa ,  Hisashi Yasueda ,  Katsuya Seguro ,  Masao Motoki

    发明人： Kohichiro Sano ,  Yoshiyuki Kumazawa ,  Hisashi Yasueda ,  Katsuya Seguro ,  Masao Motoki

    IPC分类号： C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12N1/00 ,  C12N1/19 ,  C12N5/10 ,  C12N9/12 ,  C12N15/63 ,  C12P21/00

    CPC分类号： C12N9/1044

    摘要： The present invention relates to a transglutaminase originating from Crassostrea gigas, a gene coding for the transglutaminase, a plasmid carrying the gene, a microorganism transformed with the plasmid, a method for producing an intended transglutaminase by cultivating the microorganism and a method for gelating a protein using the transglutaminase originating from Crassostrea gigas. When comparing with other transglutaminases, the transglutaminase originating from Crassostrea gigas has novel characteristic properties such that it can be activated by the action of calcium ions and that it is further activated by the addition of sodium chloride and/or potassium chloride and it is of utility value, in particular, as a gelling agent for foods.

    摘要翻译： PCT No.PCT / JP95 / 00117 Sec。 371 1995年9月28日第 102（e）1995年9月28日PCT PCT 1995年1月30日PCT公布。 公开号WO95 / 20662 日期1995年8月3日本发明涉及来自Crassostrea gigas的转谷氨酰胺酶，编码转谷氨酰胺酶的基因，携带该基因的质粒，用质粒转化的微生物，通过培养微生物生产预期的转谷氨酰胺酶的方法和 使用源自Crassostrea gigas的转谷氨酰胺凝胶化蛋白质的方法。 当与其他转谷氨酰胺酶相比时，源自Crassostrea gigas的转谷氨酰胺酶具有新颖的特征，使其可以通过钙离子的作用而被活化，并且通过加入氯化钠和/或氯化钾进一步活化，并且其具有效用 价值，特别是作为食品的胶凝剂。

    SWEETENER
    2.
    发明申请
    SWEETENER 有权

    公开(公告)号：US20100183792A1

    公开(公告)日：2010-07-22

    申请号：US12613615

    申请日：2009-11-06

    申请人： Hiroaki Nagasaki ,  Naohiro Miyamura ,  Yuzuru Eto ,  Katsuya Seguro

    发明人： Hiroaki Nagasaki ,  Naohiro Miyamura ,  Yuzuru Eto ,  Katsuya Seguro

    IPC分类号： A23L1/236

    CPC分类号： A23L27/31 ,  A23L27/00 ,  A23L27/32 ,  A23V2002/00 ,  A23V2200/16 ,  A23V2250/06 ,  A23V2250/2482 ,  A23V2250/264 ,  A23V2250/242

    摘要： The body taste of a sweetener (for example, aspartame, sucralose, acesulfame, etc.) is improved by blending the sweetener with an amino acid or a peptide, which is able to activate a calcium receptor, such as γ-Glu-X-Gly (wherein X represents an amino acid or an amino acid derivative), γ-Glu-Val-Y (wherein Y represents an amino acid or an amino acid derivative), γ-Glu-Ala, γ-Glu-Gly, γ-Glu-Cys, γ-Glu-Met, γ-Glu-Thr, γ-Glu-Val, γ-Glu-Orn, Asp-Gly, Cys-Gly, Cys-Met, Glu-Cys, Gly-Cys, Leu-Asp, D-Cys, γ-Glu-Met(O), γ-Glu-γ-Glu-Val, γ-Glu-Val-NH2, γ-Glu-Val-ol, γ-Glu-Ser, γ-Glu-Tau, γ-Glu-Cys(S-Me)(O), γ-Glu-Leu, γ-Glu-Ile, γ-Glu-t-Leu, γ-Glu-Cys(S-Me), etc.

    摘要翻译： 甜味剂（例如，阿斯巴甜，三氯蔗糖，乙酰磺胺酸等）的身体味道通过将甜味剂与能够激活钙受体的氨基酸或肽混合来改进，所述钙受体例如γ-Glu-X- Gly（其中X表示氨基酸或氨基酸衍生物），γ-Glu-Val-Y（其中Y表示氨基酸或氨基酸衍生物），γ-Glu-Ala，γ-Glu-Gly， Glu-Cys，γ-Glu-Met，γ-Glu-Thr，γ-Glu-Val，γ-Glu-Orn，Asp-Gly，Cys-Gly，Cys-Met，Glu-Cys，Gly-Cys， Asp，D-Cys，γ-Glu-Met（O），γ-Glu-γ-Glu-Val，γ-Glu-Val-NH2，γ-Glu-Val-ol，γ-Glu-Ser， -Tau，γ-Glu-Cys（S-Me）（O），γ-Glu-Leu，γ-Glu-Ile，γ-Glu-t-Leu，γ-Glu-Cys（S-Me）

    Sweetener
    6.
    发明授权
    Sweetener 有权
    甜味剂

    公开(公告)号：US09420813B2

    公开(公告)日：2016-08-23

    申请号：US12613615

    申请日：2009-11-06

    申请人： Hiroaki Nagasaki ,  Naohiro Miyamura ,  Yuzuru Eto ,  Katsuya Seguro

    发明人： Hiroaki Nagasaki ,  Naohiro Miyamura ,  Yuzuru Eto ,  Katsuya Seguro

    IPC分类号： A23L1/236 ,  A23L1/22

    CPC分类号： A23L27/31 ,  A23L27/00 ,  A23L27/32 ,  A23V2002/00 ,  A23V2200/16 ,  A23V2250/06 ,  A23V2250/2482 ,  A23V2250/264 ,  A23V2250/242

    摘要： The body taste of a sweetener (for example, aspartame, sucralose, acesulfame, etc.) is improved by blending the sweetener with an amino acid or a peptide, which is able to activate a calcium receptor, such as γ-Glu-X-Gly (wherein X represents an amino acid or an amino acid derivative), γ-Glu-Val-Y (wherein Y represents an amino acid or an amino acid derivative), γ-Glu-Ala, γ-Glu-Gly, γ-Glu-Cys, γ-Glu-Met, γ-Glu-Thr, γ-Glu-Val, γ-Glu-Orn, Asp-Gly, Cys-Gly, Cys-Met, Glu-Cys, Gly-Cys, Leu-Asp, D-Cys, γ-Glu-Met(O), γ-Glu-γ-Glu-Val, γ-Glu-Val-NH2, γ-Glu-Val-ol, γ-Glu-Ser, γ-Glu-Tau, γ-Glu-Cys(S-Me)(O), γ-Glu-Leu, γ-Glu-Ile, γ-Glu-t-Leu, γ-Glu-Cys(S-Me), etc.

    摘要翻译： 甜味剂（例如，阿斯巴甜，三氯蔗糖，乙酰磺胺酸等）的身体味道通过将甜味剂与能够激活钙受体的氨基酸或肽混合来改进，所述钙受体例如γ-Glu-X- Gly（其中X表示氨基酸或氨基酸衍生物），γ-Glu-Val-Y（其中Y表示氨基酸或氨基酸衍生物），γ-Glu-Ala，γ-Glu-Gly， Glu-Cys，γ-Glu-Met，γ-Glu-Thr，γ-Glu-Val，γ-Glu-Orn，Asp-Gly，Cys-Gly，Cys-Met，Glu-Cys，Gly-Cys， Asp，D-Cys，γ-Glu-Met（O），γ-Glu-γ-Glu-Val，γ-Glu-Val-NH2，γ-Glu-Val-ol，γ-Glu-Ser， -Tau，γ-Glu-Cys（S-Me）（O），γ-Glu-Leu，γ-Glu-Ile，γ-Glu-t-Leu，γ-Glu-Cys（S-Me）

    Process for producing microbial transglutaminase
    7.
    发明授权
    Process for producing microbial transglutaminase 有权
    微生物转谷氨酰胺酶的生产工艺

    公开(公告)号：US06821763B2

    公开(公告)日：2004-11-23

    申请号：US09884948

    申请日：2001-06-21

    申请人： Keiichi Yokoyama ,  Nami Nakamura ,  Tetsuya Miwa ,  Katsuya Seguro

    发明人： Keiichi Yokoyama ,  Nami Nakamura ,  Tetsuya Miwa ,  Katsuya Seguro

    IPC分类号： C12N910

    CPC分类号： C12N9/1044

    摘要： Disclosed are a protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1, a DNA encoding the protein, a transformant having the DNA, and a process for producing a protein having a transglutaminase activity, which comprises the steps of culturing the transformant in a medium. The protein can be produced in a large amount with the transformant using a host such as E. coli.

    摘要翻译： 公开了具有转谷氨酰胺酶活性的蛋白质，其包含从SEQ ID No.1所示的氨基酸序列中的第二位置的丝氨酸残基至第331位的脯氨酸残基的序列，其中蛋白质的N-末端氨基酸 对应于SEQ ID No.1的第二位的丝氨酸残基，编码该蛋白质的DNA，具有该DNA的转化体，以及具有转谷氨酰胺酶活性的蛋白质的制造方法，其包括在培养基中培养转化体的步骤 。 使用宿主例如大肠杆菌可以用转化体大量生产蛋白质。

    Process for producing microbial transglutaminase
    8.
    发明授权
    Process for producing microbial transglutaminase 有权

    公开(公告)号：US06538122B1

    公开(公告)日：2003-03-25

    申请号：US09448310

    申请日：1999-11-24

    申请人： Keiichi Yokoyama ,  Nami Nakamura ,  Tetsuya Miwa ,  Katsuya Seguro

    发明人： Keiichi Yokoyama ,  Nami Nakamura ,  Tetsuya Miwa ,  Katsuya Seguro

    IPC分类号： C12N1554

    CPC分类号： C12N9/1044

    摘要： Disclosed are a protein having a transglutaminase activity, which comprises a sequence ranging from serine residue at the second position to proline residue at the 331st position in an amino acid sequence represented by SEQ ID No. 1 wherein the N-terminal amino acid of the protein corresponds to serine residue at the second position of SEQ ID No. 1, a DNA encoding the protein, a transformant having the DNA, and a process for producing a protein having a transglutaminase activity, which comprises the steps of culturing the transformant in a medium. The protein can be produced in a large amount with the transformant using a host such as E. coli.