摘要:
The present invention is to provide a plant and a plant storage organ thereof in which GLP-1 derivatives are accumulated, and a method of producing them in order to develop a method for ingesting orally GLP-1 derivatives at a low cost and to make use of it in diabetic treatment. A transgenic plant or a plant storage organ thereof in which GLP-1 derivatives are accumulated cleavable with a digestive enzyme is produced by a method comprising: integrating into a vector a linked GLP-1s-DNA which comprises tandem repeated “n” DNAs (“n” being an integer of 3 or more) encoding a GLP-1 derivative consisting of GLP-1 (7-36) or of an amino acid sequence of GLP-1 (7-36) in which one or a few amino acids are deleted, substituted and/or added, and C-terminal consists of Arg or Lys, and having GLP-1 activity; introducing the vector into a plant cell; and redifferentiating the obtained transformant. The transgenic plant and the plant storage organ are useful as a pharmaceutical composition or a food or drink for treating or preventing diabetes.
摘要:
The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要:
The present invention is regarding plants and plant storage organs thereof in which GLP-1 derivatives are accumulated, and methods of producing them. The transgenic plants and plant storage organs thereof accumulate tandem repeated GLP-1 derivatives cleavable with intestinal digestive enzyme to monomeric molecules and are produced by methods comprising: integrating into vectors linked DNAs which comprise tandem repeated DNAs encoding the GLP-1 derivative with trypsin resistance in which the amino acid in the 26th position is Gln, the amino acid in the 34th position is Asn or Asp, and C-terminal consists of Arg or Lys to produce monomeric molecules; introducing the vectors into plant cells; and redifferentiating the obtained transformants. The edible transgenic plants and plant storage organs are useful for treating diabetes and can be ingested by diabetic patients.
摘要:
The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要:
To provide a transgenic rice plant that can produce rice which can be used as an “edible vaccine”, i.e., rice capable of inducing a desired immune response when mucosally administered such as an oral administration. There is provided a transgenic rice plant including a genomic DNA, wherein a DNA construct is incorporated into the genomic DNA so that the DNA construct is capable of being expressed, wherein the DNA construct includes a DNA encoding an antigenic protein, and a rice endosperm specific promoter linked upstream thereof.
摘要:
The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要:
It is an object of the present invention to provide a protein that can successfully treats almost all patients with cedar pollinosis and can be ingested as a food. That is, the present invention provides a protein comprising an amino acid sequence represented by SEQ ID NO:1 or comprising an amino acid sequence having a mutation(s) in said amino acid sequence, and having an immunogenicity of a cedar pollen; a polynucleotide encoding said protein; a vector comprising said polynucleotide; a transformant comprising said polynucleotide or said vector; and intended uses of said protein, said polynucleotide, and said transformant.
摘要翻译:本发明的目的是提供一种可以成功地治疗几乎所有患有雪松花粉症的患者的蛋白质,并且可以作为食物摄取。 也就是说,本发明提供了包含由SEQ ID NO:1所示的氨基酸序列或包含在所述氨基酸序列中具有突变并具有雪松花粉免疫原性的氨基酸序列的蛋白质的蛋白质。 编码所述蛋白质的多核苷酸; 包含所述多核苷酸的载体; 包含所述多核苷酸或所述载体的转化体; 和所述蛋白质,所述多核苷酸和所述转化体的预期用途。
摘要:
It is an object of the present invention to provide a protein that can successfully treats almost all patients with cedar pollinosis and can be ingested as a food. That is, the present invention provides a protein comprising an amino acid sequence represented by SEQ ID NO:1 or comprising an amino acid sequence having a mutation(s) in said amino acid sequence, and having an immunogenicity of a cedar pollen; a polynucleotide encoding said protein; a vector comprising said polynucleotide; a transformant comprising said polynucleotide or said vector; and intended uses of said protein, said polynucleotide, and said transformant.
摘要翻译:本发明的目的是提供一种可以成功地治疗几乎所有患有雪松花粉症的患者的蛋白质,并且可以作为食物摄取。 也就是说,本发明提供了包含由SEQ ID NO:1所示的氨基酸序列或包含在所述氨基酸序列中具有突变并具有雪松花粉免疫原性的氨基酸序列的蛋白质的蛋白质。 编码所述蛋白质的多核苷酸; 包含所述多核苷酸的载体; 包含所述多核苷酸或所述载体的转化体; 和所述蛋白质,所述多核苷酸和所述转化体的预期用途。
摘要:
The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
摘要:
The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.