摘要:
A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要:
A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要:
A method of isolating nucleic acid from a sample containing nucleic acid is provided. The method includes contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of the nucleic acid to the bifunctional material; and extracting the nucleic acid at a second pH higher than the first pH from the complex.
摘要:
Provided are nucleic acid isolation unit and method. The method includes immobilizing an aromatic compound-containing nucleic acid intercalator on a solid support; contacting a first buffer solution containing a nucleic acid sample to be purified to the intercalator immobilized on the solid support to bind the intercalator with nucleic acids contained in the nucleic acid sample; cleaning the resultant structure where the nucleic acids are bound to the intercalator immobilized on the solid support; and eluting the nucleic acids with a second buffer solution.
摘要:
Provided is a method of preparing a patterned spot microarray using a photocatalyst. The method comprises coating the photocatalyst on a substrate to form a photocatalyst layer, coating a composition comprising a functional group to be connected to a biomolecule on the photocatalyst layer to form an organic layer, spotting the biomolecule on the organic layer, positioning a photomask above a spot of the biomolecule; and irradiating the spot through the photomask to pattern the spot.
摘要:
Provided are a method of producing a substrate having a patterned organosilane layer and a method of using the substrate having the patterned organosilane layer. The method of producing the substrate having the patterned organosilane layer, includes: coating organosilane on a substrate to obtain an organosilane layer; coating a photoresist material on the organosilane layer; exposing the photoresist material to light through a mask to obtain a patterned surface on the photoresist material; developing an exposed or unexposed region of the photoresist material using a developer; and wet etching a portion of the organosilane layer in the region from which the photoresist material has been removed, using a HF-containing solution as an etchant.
摘要:
Provided are a method of producing a substrate having a patterned organosilane layer and a method of using the substrate having the patterned organosilane layer. The method of producing the substrate having the patterned organosilane layer, includes: coating organosilane on a substrate to obtain an organosilane layer; coating a photoresist material on the organosilane layer; exposing the photoresist material to light through a mask to obtain a patterned surface on the photoresist material; developing an exposed or unexposed region of the photoresist material using a developer; and wet etching a portion of the organosilane layer in the region from which the photoresist material has been removed, using a HF-containing solution as an etchant.
摘要:
A method of manufacturing a DNA (deoxyribonucleic acid) chip is provided. The DNA chip has a plurality of transistors formed on a substrate and an organic layer and a DNA probe sequentially stacked on a gate of the transistor. The method includes forming an inter-layer insulation layer on the substrate to cover the transistors, planarizing the inter-layer insulation layer, forming at least two contact holes exposing gate electrodes of the transistors in the inter-layer insulation layer, selectively forming organic layers on the exposed gate electrodes, attaching a first DFR (dry film resist) layer to the upper surface of the inter-layer insulation layer to cover the contact holes, removing a portion of the first DFR layer covering a first contact hole among the contact holes, attaching a first DNA probe to the organic layers in the first contact hole, and removing a remaining portion of the first DFR layer.
摘要:
Provided is a method of storing a substrate having an active group or a probe molecule immobilized thereon. The method includes attaching a UV film to a surface of the substrate having the active group or probe immobilized thereon; and exposing the UV film to UV light, wherein the UV film comprises a UV-permeable base film and a pressure sensitive adhesive layer formed on a surface of the UV-permeable base film to immobilize the substrate, and when the pressure sensitive adhesive layer is exposed to the UV light, its adhesive force decreases.
摘要:
Provided is a method of storing a substrate having an active group or a probe molecule immobilized thereon. The method includes attaching a UV film to a surface of the substrate having the active group or probe immobilized thereon; and exposing the UV film to UV light, wherein the UV film comprises a UV-permeable base film and a pressure sensitive adhesive layer formed on a surface of the UV-permeable base film to immobilize the substrate, and when the pressure sensitive adhesive layer is exposed to the UV light, its adhesive force decreases.