公开(公告)号：US11530455B2

公开(公告)日：2022-12-20

申请号：US17099621

申请日：2020-11-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chang Zhong

IPC: C12Q1/6888 ,  C12Q1/6881 ,  C12Q1/6858

Abstract: Methods for human identification using polymorphisms in the Penta E short tandem repeat locus are significant in preventing allelic drop out. An exemplary method encompasses (a) contacting a first primer to a nucleic acid sample to be analyzed, (b) contacting a second primer to the nucleic acid sample, and (c) subjecting the nucleic acid sample, the first primer, and the second primer to an amplification reaction, and thereby forming an amplification product. The first primer, the second primer, or both the first and second primers can be labeled with a non-nucleic-acid label. Additionally, or alternatively, the amplification product can include an adenosine at position 14 from the 5′ end of SEQ ID NO:1, a thymidine at position 21 from the 5′ end of SEQ ID NO:2, or both an adenine at position 14 and a thymidine at position 21 from the 5′ end of SEQ ID NO:3.

公开(公告)号：US11293059B2

公开(公告)日：2022-04-05

申请号：US16623975

申请日：2018-06-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chang Zhong ,  Julio Mulero ,  Steven Menchen

IPC: C12Q1/686 ,  C12Q1/6848

Abstract: Disclosed herein are mesylate containing compositions, methods of use of such compositions and kits of components encompassing such compositions for improving the amplification of nucleic acids, especially in the presence of enzymatic inhibitors. These find particular use in forensic and environmental analyses. The instant disclosure provides new compositions, methods and kits of components for overcoming PCR inhibition. In some embodiments disclosed herein is a PCR master mix, the PCR master mix encompassing a mesylate.

公开(公告)号：US09804066B2

公开(公告)日：2017-10-31

申请号：US14739899

申请日：2015-06-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael Harrold ,  Lori Hennessy ,  Jason Yingjie Liu ,  Chang Zhong

IPC: C12Q1/68 ,  C12M1/00 ,  C12M1/34 ,  G01N1/30 ,  G06T7/00 ,  A61B5/15 ,  A61B5/151 ,  A61B5/00 ,  A61B5/1172 ,  A61B5/145 ,  G06K9/00 ,  A61B10/00 ,  A61B10/02 ,  A61B5/157

CPC classification number: G01N1/30 ,  A61B5/0077 ,  A61B5/1172 ,  A61B5/1411 ,  A61B5/14514 ,  A61B5/150022 ,  A61B5/150083 ,  A61B5/150091 ,  A61B5/150412 ,  A61B5/150984 ,  A61B5/15105 ,  A61B5/15134 ,  A61B5/157 ,  A61B10/0064 ,  A61B10/0233 ,  G06K9/00013 ,  G06K9/00543 ,  G06K2009/00946 ,  G06T7/0012

Abstract: Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individual's identity or determine the identity of an individual.

公开(公告)号：US09708644B2

公开(公告)日：2017-07-18

申请号：US13774903

申请日：2013-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu ,  Michael Harrold ,  Chang Zhong ,  Chandani Patel

IPC: C12M1/00 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  G01N31/22 ,  G06K9/00 ,  G03B29/00 ,  G01N1/30 ,  A61B10/00 ,  A61B10/02

CPC classification number: C12Q1/6806 ,  A61B10/0045 ,  A61B10/02 ,  G01N1/30

Abstract: Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individual's identity or determine the identity of an individual.