公开(公告)号：US10710081B2

公开(公告)日：2020-07-14

申请号：US15125205

申请日：2015-03-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: B01L3/00

Abstract: Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.

公开(公告)号：US20160348092A1

公开(公告)日：2016-12-01

申请号：US15235327

申请日：2016-08-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  C12N1/06 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

Abstract translation: 本教导涉及从固体材料中提取核酸。 提供了用于从固体生物样品获得核酸的有用的组合物，方法和试剂盒或具有粘附或嵌入粘合剂基底内的生物材料的粘合剂材料。 提取的核酸可用于下游应用，例如生物材料来源的基因分型，检测，定量和鉴定。

公开(公告)号：US11820978B2

公开(公告)日：2023-11-21

申请号：US17146163

申请日：2021-01-11

Applicant: Life Technologies Corporation

Inventor： James Stray ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12Q1/6806 ,  C12N15/10 ,  C12N1/06

CPC classification number: C12N15/1003 ,  C12N1/06 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

公开(公告)号：US11643680B2

公开(公告)日：2023-05-09

申请号：US17209957

申请日：2021-03-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12P19/34 ,  C12Q1/6851 ,  C12Q1/6806 ,  C12Q1/686

CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2561/113 ,  C12Q2565/607 ,  C12Q2565/625

Abstract: A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13 pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.

公开(公告)号：US10894957B2

公开(公告)日：2021-01-19

申请号：US16013938

申请日：2018-06-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James Stray ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10 ,  C12N1/06 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

公开(公告)号：US20150024400A1

公开(公告)日：2015-01-22

申请号：US14380543

申请日：2013-02-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  A61B10/0045 ,  A61B10/02 ,  G01N1/30

Abstract: Devices, methods, and kits are disclosed for collection, labeling and analysis of samples containing a substance of interest. Such devices and methods are used in forensic, human identification, access and importation control, and other investigative technologies to collect even limited amounts of a substance of interest that may be present on a substrate and to facilitate analysis to identify the substance of interest.

Abstract translation: 公开了用于收集，标记和分析含有感兴趣物质的样品的装置，方法和试剂盒。 这些装置和方法用于法医，人类鉴定，进入和进口控制以及其他调查技术，以收集甚至有限量的可能存在于底物上的感兴趣物质，并促进分析以鉴定感兴趣的物质。

公开(公告)号：US20240200053A1

公开(公告)日：2024-06-20

申请号：US18379480

申请日：2023-10-12

Applicant: Life Technologies Corporation

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10 ,  C12N1/06 ,  C12Q1/6806

CPC classification number: C12N15/1003 ,  C12N1/06 ,  C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

公开(公告)号：US20210198655A1

公开(公告)日：2021-07-01

申请号：US17146163

申请日：2021-01-11

Applicant: Life Technologies Corporation

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10 ,  C12N1/06 ,  C12Q1/6806

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

公开(公告)号：US20180371449A1

公开(公告)日：2018-12-27

申请号：US16013938

申请日：2018-06-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James STRAY ,  Jason Yingjie Liu ,  Maxim Brevnov ,  Jaiprakash Shewale ,  Allison Holt

IPC: C12N15/10 ,  C12Q1/6806 ,  C12N1/06

Abstract: The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

公开(公告)号：US09453264B2

公开(公告)日：2016-09-27

申请号：US14736922

申请日：2015-06-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason Yingjie Liu

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2531/113 ,  C12Q2561/113

Abstract: Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded.