公开(公告)号：US07141385B2

公开(公告)日：2006-11-28

申请号：US10346443

申请日：2003-01-17

申请人： Lawrence A. Bottomley ,  Madhushree Ghosh ,  Shanxiang Shen ,  Richard Saul ,  Sebastian Kossek ,  Gary W. Pace

发明人： Lawrence A. Bottomley ,  Madhushree Ghosh ,  Shanxiang Shen ,  Richard Saul ,  Sebastian Kossek ,  Gary W. Pace

IPC分类号： G01N33/573 ,  G01N33/542 ,  G01N21/00 ,  G01N21/29 ,  G11N33/53

CPC分类号： C12Q1/00 ,  G01N33/54373

摘要： An apparatus and a method are provided for detecting an enzyme, or or for detecting a substrate of an enzyme or for detecting an enzyme effector such as an inhibitor, by measuring a change in deflection of a microcantilever having a substrate or an enzyme, respectively, attached to a surface of the microcantilever.

摘要翻译： 分别通过测量具有底物或酶的微型悬臂梁的偏转变化，提供了用于检测酶，或或用于检测酶的底物或用于检测酶效应物如抑制剂的装置和方法， 附着于微悬臂梁的表面。

公开(公告)号：US20050208586A1

公开(公告)日：2005-09-22

申请号：US10803246

申请日：2004-03-18

申请人： Shanxiang Shen

发明人： Shanxiang Shen

IPC分类号： C07K16/46 ,  G01N33/53 ,  G01N33/542 ,  G01N33/564

CPC分类号： B82Y30/00 ,  B82Y5/00 ,  B82Y10/00 ,  G01N33/542 ,  G01N33/564 ,  G01N2333/4716

摘要： The present invention relates to polypeptides or non-polypeptides derived from C1q, a subunit of the first complement component molecule C1. These molecules bind to the C1q receptor on the Fc domain of an antibody in immune complexes (also called antigen-antibody complexes or aggregated immunoglobulins), but do not bind to free immunoglobulins. These complement component derived molecules may be used as tracer molecules for fluorescence polarization (FP). The present invention also relates to materials and methods of producing molecules for use in FP. The present invention has many applications in the areas of biosensor development for proteomics research, protein expression profiling, drug discovery, diagnosis and prognosis, monitoring therapeutic effects, environmental survey, and bio-defense.

摘要翻译： 本发明涉及来自第一补体成分分子C1的亚单位C1q的多肽或非多肽。 这些分子与免疫复合物（也称为抗原 - 抗体复合物或聚集的免疫球蛋白）中抗体的Fc结构域上的C1q受体结合，但不结合游离的免疫球蛋白。 这些补体成分衍生的分子可以用作荧光偏振（FP）的示踪剂分子。 本发明还涉及生产用于FP的分子的材料和方法。 本发明在蛋白质组学研究的生物传感器开发，蛋白质表达谱，药物发现，诊断和预后，监测治疗效果，环境调查和生物防御领域具有很多应用。