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    Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme
    3.
    发明授权
    Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme 有权
    使用耐热TAL酶制备对羟基肉桂酸的方法

    公开(公告)号：US07572612B2

    公开(公告)日：2009-08-11

    申请号：US11485558

    申请日：2006-07-12

    Applicant: Lixuan Lisa Huang ,  Zhixiong Xue ,  Michael P. McCluskey

    Inventor： Lixuan Lisa Huang ,  Zhixiong Xue ,  Michael P. McCluskey

    IPC: C12P7/42 ,  C12N1/20

    CPC classification number: C12N9/88 ,  C12P7/42

    Abstract: A thermostable TAL enzyme was identified from the fungus Phanerochaete chrysosporium, which has high activity at temperatures of 40° C. to about 60° C. The enzyme was produced in engineered cells and used for production of para-hydroxycinnamic acid (pHCA) from tyrosine. When the pHCA production reaction was run at high temperature, the enzyme was more active and pHCA was produced more rapidly, making the reaction more efficient.

    Abstract translation: 从真菌Phanerochaete chrysosporium鉴定出热稳定的TAL酶，其在40℃至约60℃的温度下具有高活性。该酶在工程细胞中产生，用于从酪氨酸生产对羟基肉桂酸（pHCA） 。 当pHCA生产反应在高温下运行时，酶活性更高，pHCA产生更快，使反应更有效。

    Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme
    4.
    发明申请
    Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme 有权
    使用耐热TAL酶制备对羟基肉桂酸的方法

    公开(公告)号：US20080213846A1

    公开(公告)日：2008-09-04

    申请号：US11485558

    申请日：2006-07-12

    Applicant: Lixuan Lisa Huang ,  Michael P. McCluskey ,  Zhixiong Xue

    Inventor： Lixuan Lisa Huang ,  Michael P. McCluskey ,  Zhixiong Xue

    IPC: C12P7/42 ,  C12N1/20

    CPC classification number: C12N9/88 ,  C12P7/42

    Abstract: A thermostable TAL enzyme was identified from the fungus Phanerochaete chrysosporium, which has high activity at temperatures of 40° C. to about 60° C. The enzyme was produced in engineered cells and used for production of para-hydroxycinnamic acid (pHCA) from tyrosine. When the pHCA production reaction was run at high temperature, the enzyme was more active and pHCA was produced more rapidly, making the reaction more efficient.

    Abstract translation: 从真菌Phanerochaete chrysosporium鉴定出热稳定的TAL酶，其在40℃至约60℃的温度下具有高活性。该酶在工程细胞中产生，用于从酪氨酸生产对羟基肉桂酸（pHCA） 。 当pHCA生产反应在高温下运行时，酶活性更高，pHCA产生更快，使反应更有效。

    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
    7.
    发明授权
    Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production 有权
    优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产

    公开(公告)号：US08323935B2

    公开(公告)日：2012-12-04

    申请号：US12244822

    申请日：2008-10-03

    Applicant: Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    Inventor： Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

    IPC: C12P7/64 ,  C07D293/10

    CPC classification number: C12P7/6427 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/815 ,  C12P7/6472

    Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are described, as are methods for producing EPA within the host cells and products comprising EPA from the optimized Yarrowia lipolytica strains.

    Abstract translation: 描述了能够在总油馏分中产生大于50重量％的二十碳五烯酸[EPA]（ω-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株过表达异源＆Dgr。9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱氢酶，Dgr。12去饱和酶和C16 / 18延伸酶，以及任选的过表达二酰基甘油胆碱磷酸转移酶。 描述优选的基因敲除，以及在宿主细胞内生产EPA的方法以及来自优化的解脂耶氏酵母菌株的包含EPA的产物的方法。

    PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS
    9.
    发明申请
    PENTOSE PHOSPHATE PATHWAY UPREGULATION TO INCREASE PRODUCTION OF NON-NATIVE PRODUCTS OF INTEREST IN TRANSGENIC MICROORGANISMS 审中-公开
    PENTOSE磷酸盐路径升级以增加转基因微生物中非生物利益产品的生产

    公开(公告)号：US20110244512A1

    公开(公告)日：2011-10-06

    申请号：US13074069

    申请日：2011-03-29

    Applicant: Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    Inventor： Seung-Pyo Hong ,  Zhixiong Xue ,  Quinn Qun Zhu

    IPC: C12P1/00 ,  C12N1/00 ,  C12N1/13 ,  C12N1/19 ,  C12N1/15

    CPC classification number: C12P7/6427 ,  C12N9/0006 ,  C12N9/18 ,  C12N15/52 ,  C12P7/6472 ,  C12P7/66 ,  C12P23/00 ,  C12P33/00

    Abstract: Coordinately regulated over-expression of the genes encoding glucose 6-phosphate dehydrogenase [“G6PDH”] and 6-phospho-gluconolactonase [“6PGL”] in transgenic strains of the oleaginous yeast, Yarrowia lipolytica, comprising a functional polyunsaturated fatty acid [“PUFA”] biosynthetic pathway, resulted in increased production of PUFAs and increased total lipid content in the Yarrowia cells. This is achieved by increased cellular availability of the reduced form of nicotinamide adenine dinucleotide phosphate [“NADPH”], an important reducing equivalent for reductive biosynthetic reactions, within the transgenic microorganism.

    Abstract translation: 协调调节编码葡萄糖6-磷酸脱氢酶[“G6PDH”]和6-磷酸 - 葡糖酸半乳糖醛酸酶[“6PGL”]的基因在含油酵母，解脂耶氏酵母的转基因菌株中的过表达，其包含功能性多不饱和脂肪酸[“PUFA “]生物合成途径导致了PUFA的生产增加，并增加了耶氏酵母细胞中的总脂质含量。 这是通过增加转基因微生物中烟酰胺腺嘌呤二核苷酸磷酸盐还原形式的细胞可利用度[“NADPH”]，这是还原性生物合成反应的重要还原等价物。

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