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公开(公告)号：US20240384332A1
公开(公告)日：2024-11-21
申请号：US18393023
申请日：2023-12-21
Applicant: Mammoth Biosciences, Inc.
Inventor： Jesus CHING , James Paul BROUGHTON , Janice Sha CHEN , Clare Louise FASCHING , Phillip You Fai LEE , Brian J. EASTMAN , Devin SPRATT , Sarah Jane SHAPIRO , Sonal JAIN , Matthew VEROSLOFF , Jasmeet SINGH , Elizabeth M. HAWKINS , Vaishnavi NAGESH , Lior KREINDLER , Sophia HUBBELL , Carley Gelenter HENDRIKS , Emma G. STANFIELD , Amanda Claire BROWN
IPC: C12Q1/6844 , B01L3/00 , C12Q1/34
Abstract: Various compositions and diagnostic devices for the detection of nucleic acids are provided. Also provided are systems comprising the same, and methods of using the same. In some embodiments, compositions and devices comprise or are configured to be used in combination with one or more of a guide nucleic acid, a programmable nuclease, and reporters. In some embodiments, presence of at least one sequence of interest is determined by detecting a signal produced upon cleavage of the reporters.
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公开(公告)号：US20240359180A1
公开(公告)日：2024-10-31
申请号：US18541650
申请日：2023-12-15
Applicant: Mammoth Biosciences, Inc.
Inventor： Timothy James PATNO , Phillip You Fai LEE , Benjamin Andrew BLIZARD , Xin MIAO , Jacob LESINSKI , Ryan A. BROWN , Daniel Thomas DRZAL , Sarah Jane SHAPIRO , Joshua BAIK , Janice Sha CHEN , James Paul BROUGHTON , Clare Louise FASCHING , Jesus CHING , Sonal JAIN , Deepika VERMA , Matthew VEROSLOFF , Devin SPRATT , Nicholas John FANTIN
IPC: B01L3/00
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0684 , B01L2200/10 , B01L2200/16 , B01L2300/0663 , B01L2300/161 , B01L2300/1805 , B01L2400/06
Abstract: The present disclosure provides various systems, diagnostic devices, and methods for nucleic acid analysis. The systems, devices, and methods allow for the analysis of nucleic acids, in a sample, via programmable nuclease-based assays. Provided are systems comprising an instrument and a cartridge allowing for point of care use. The systems, devices, and methods described herein can be configured for multiplexed detection of nucleic acids in a single sample.
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公开(公告)号：US20240294970A1
公开(公告)日：2024-09-05
申请号：US18334327
申请日：2023-06-13
Applicant: Mammoth Biosciences, Inc.
Inventor： Jesus CHING , James Paul BROUGHTON , Janice Sha CHEN , Sarah Jane SHAPIRO , Elizabeth Mae HAWKINS , Sonal JAIN , Lior KREINDLER , Sophia HUBBELL
IPC: C12Q1/6823 , C12N15/10 , C12Q1/44 , C12Q1/6806 , C12Q1/6844 , G01N21/64
CPC classification number: C12Q1/6823 , C12N15/1096 , C12Q1/44 , C12Q1/6806 , C12Q1/6844 , G01N21/6428 , G01N2021/6432 , G01N2333/922
Abstract: Described herein are methods, devices, and compositions for immobilization of biomolecules in diagnostic assays onto a surface. The methods, devices, and compositions may utilize or comprise a plurality of different non-naturally occurring guide nucleic acids. Each of the different non-naturally occurring guide nucleic acids may be immobilized to a surface at a known location identified with the particular non-naturally occurring guide nucleic acid, Alternatively, or in combination, a plurality of programmable nucleases may be immobilized to the surface at each of the known locations. Optionally, the methods, devices, and compositions may utilize or comprise a plurality of reporters immobilized to the surface in proximity to each of the different non-naturally occurring guide nucleic acids and/or programmable nucleases at each of the known locations.
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公开(公告)号：US20240209421A1
公开(公告)日：2024-06-27
申请号：US18334320
申请日：2023-06-13
Applicant: Mammoth Biosciences, Inc.
Inventor： Janice Sha CHEN , Carley Gelenter HENDRIKS , Clare Louise FASCHING , Xin MIAO , Nazmiye Emel ALPAY , James Paul BROUGHTON , Elizabeth M. HAWKINS , Matthew S. VEROSLOFF , Sophia HUBBELL , Sarah Jane SHAPIRO , Lior KREINDLER , Jesus CHING
IPC: C12Q1/6823 , C12N9/22 , C12N15/11 , C12Q1/44 , C12Q1/48 , C12Q1/6844
CPC classification number: C12Q1/6823 , C12N9/22 , C12N15/111 , C12Q1/44 , C12Q1/485 , C12Q1/6844 , C12N2310/20 , G01N2333/9126 , G01N2333/9128 , G01N2333/922
Abstract: The present disclosure provides single-buffer systems that enable efficient and rapid amplification and programmable nuclease enzyme mediated reactions. The single-buffer systems and reagent compositions therein may be used for assaying for a nucleic acid sequence from a sample.
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公开(公告)号：US20230332253A1
公开(公告)日：2023-10-19
申请号：US17817889
申请日：2022-08-05
Applicant: Mammoth Biosciences, Inc.
Inventor： Maria-Nefeli TSALOGLOU , Clare FASCHING , Jesus CHING , Janice Sha CHEN , James Paul BROUGHTON , Daniel Thomas DRZAL , Sarah Jane SHAPIRO
IPC: C12Q1/70 , C12Q1/6853 , B01L3/00 , B01L7/00
CPC classification number: C12Q1/701 , C12Q1/6853 , B01L3/502715 , B01L7/52 , C12Q2600/16 , C12Q2600/156 , B01L2300/0636 , B01L2300/087 , B01L2200/16 , B01L2300/0883
Abstract: Described herein, in certain embodiments, are methods of assaying for a segment of a coronavirus target nucleic acid in a sample. In some embodiments, the target nucleic acid is from a gene of SARS-CoV-2, or a variant thereof. In some embodiments, methods described herein comprise a) contacting the sample to: i) a detector nucleic acid; and ii) a composition comprising a programmable nuclease and a non-naturally occurring guide nucleic acid, and b) assaying for a change in a signal, wherein the change in the signal is produced by cleavage of the detector nucleic acid by the programmable nuclease. Also provided herein are devices for performing the methods of assaying for a segment of a coronavirus target nucleic acid described herein.
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公开(公告)号：US20240326048A1
公开(公告)日：2024-10-03
申请号：US18058122
申请日：2022-11-22
Applicant: MAMMOTH BIOSCIENCES, INC.
Inventor： Clare Louise FASCHING , Matthew VEROSLOFF , Jesus CHING , James Paul BROUGHTON , Maria-Nefeli TSALOGLOU , Phillip You Fai LEE , Timothy James PATNO , Janice Sha CHEN , Sarah Jane SHAPIRO , James Raymond CORDOVA , Brian J. EASTMAN , Donald Allan LOWMILLER , Lior KREINDLER , Daniel Thomas DRZAL , Devin SPRATT , Jacob LESINSKI , Joshua BAIK , Rachel Nouha DERANEY , Sonal JAIN , Sophia HUBBELL , Carley Gelenter HENDRIKS
IPC: B01L3/00 , B01L7/00 , C12N9/22 , C12N15/11 , C12Q1/48 , C12Q1/6806 , C12Q1/6823 , C12Q1/6834 , C12Q1/6844
CPC classification number: B01L3/502761 , B01L3/502715 , B01L7/52 , C12N9/22 , C12N15/111 , C12Q1/485 , C12Q1/6806 , C12Q1/6823 , C12Q1/6834 , C12Q1/6844 , B01L2200/16 , B01L2300/0681 , B01L2300/1877 , B01L2400/0487 , C12N2310/20
Abstract: The present disclosure provides various diagnostic devices. Devices can comprising a sample interface configured to receive a sample that may comprise at least one sequence of interest; a channel in fluid communication with the sample interface and a detection chamber, said channel comprising one or more movable mechanisms to separate the sample into a plurality of droplets, wherein said detection chamber is configured to receive and contact the plurality of droplets with at least one programmable nuclease probe disposed on a surface of said detection chamber, and wherein said at least one programmable nuclease probe may comprise a guide nucleic acid complexed with a programmable nuclease; and a plurality of sensors that determine a presence of said at least one sequence of interest by detecting a signal produced upon cleavage of a target nucleic acid region of said at least one sequence of interest by said at least one programmable nuclease probe.
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公开(公告)号：US20240139728A1
公开(公告)日：2024-05-02
申请号：US18055814
申请日：2022-11-15
Applicant: Mammoth Biosciences, Inc.
Inventor： Maria-Nefeli TSALOGLOU , Janice Sha CHEN , James Paul BROUGHTON , Daniel Thomas DRZAL , Sarah Jane SHAPIRO , Clare Louise FASCHING , Carley Gelenter HENDRIKS , Jesus CHING , Sonal JAIN
CPC classification number: B01L3/5023 , G01N33/5308 , B01L2300/069 , B01L2400/06
Abstract: The application provides devices, kits and methods for a streamlined lateral flow assay for sample preparation, optional amplification, and detection of a target nucleic acid using programmable nuclease reagents.
