利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

20 条记录 (耗时 0.025 秒)

    Methods, apparatus and compositions for improved measurements with optical biosensors
    1.
    发明授权
    Methods, apparatus and compositions for improved measurements with optical biosensors 失效
    用于利用光学生物传感器改进测量的方法，装置和组合物

    公开(公告)号：US07413893B2

    公开(公告)日：2008-08-19

    申请号：US10864086

    申请日：2004-06-09

    申请人： Mack J. Schermer ,  Mark Norman Bobrow ,  Philip R. Buzby ,  Thomas R. Mullinax

    发明人： Mack J. Schermer ,  Mark Norman Bobrow ,  Philip R. Buzby ,  Thomas R. Mullinax

    IPC分类号： C12Q1/68 ,  C12M1/34

    CPC分类号： G01N21/253 ,  B01L9/50 ,  G01N21/7743

    摘要： The invention provides an apparatus for interrogating an optical biosensor. The apparatus includes (a) locating means for positioning one or more optical biosensors; (b) calibration means for generating a reference measurement; (c) one or more biosensors for generating one or more sample measurements, the biosensors being substantially planar and removable from the apparatus; (d) a reading means for interrogating biosensor and calibration means, and (e) a transport means for moving one or more of a biosensor, the calibration means and the reading means, whereby the reading means is positioned to interrogate the biosensor and the calibration means.

    摘要翻译： 本发明提供一种询问光学生物传感器的装置。 该装置包括（a）用于定位一个或多个光学生物传感器的定位装置; （b）用于产生参考测量的校准装置; （c）用于产生一个或多个样品测量的一个或多个生物传感器，所述生物传感器基本上是平面的并且可从所述设备移除; （d）用于询问生物传感器和校准装置的读取装置，以及（e）用于移动生物传感器，校准装置和读取装置中的一个或多个的传送装置，由此读取装置被定位成询问生物传感器和校准 手段。

    ENZYMATIC SUBSTRATES FOR MULTIPLE DETECTION SYSTEMS
    2.
    发明申请
    ENZYMATIC SUBSTRATES FOR MULTIPLE DETECTION SYSTEMS 有权
    多种检测系统的酶基质

    公开(公告)号：US20090253117A1

    公开(公告)日：2009-10-08

    申请号：US12403790

    申请日：2009-03-13

    申请人： Blas Cerda ,  Mark Norman Bobrow

    发明人： Blas Cerda ,  Mark Norman Bobrow

    IPC分类号： C12Q1/25 ,  C07H1/00 ,  C07H5/00 ,  C08B37/00

    CPC分类号： C12Q1/34 ,  C07C237/22 ,  C07H15/203 ,  C07K5/06 ,  C07K19/00 ,  G01N2333/924 ,  G01N2458/15

    摘要： An inventive substrate is provided which includes a substrate compound of formula A-B1-B2-B3-B4: wherein A is a sugar moiety; B1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B2 is a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; B3 contains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and B4 of various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B1-B2-B3-B4, with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity.

    摘要翻译： 提供了本发明的底物，其包括式A-B1-B2-B3-B4的底物化合物：其中A是糖部分; B1是连接部分，允许部分A与底物的剩余结构共轭; B2是具有游离反应性氨基的接头部分，以便可用于与羧酸或可检测标签反应; B3含有永久带电元件如季铵基，以提高质谱分析的灵敏度; 和各种碳长度的B4在检测方法中在各个底物之间赋予特异性。 还提供了式B1-B2-B3-B4的分子，其与通过靶酶在本发明底物上的作用产生的酶产物具有相似的结构特征。 还提供了使用本发明的底物检测酶活性的方法。

    Enhanced catalyzed reporter deposition
    3.
    发明授权
    Enhanced catalyzed reporter deposition 有权
    增强催化报道沉积

    公开(公告)号：US06372937B1

    公开(公告)日：2002-04-16

    申请号：US09434742

    申请日：1999-11-05

    申请人： Mark Norman Bobrow ,  Karl Edwin Adler ,  Kevin Aaron Roth

    发明人： Mark Norman Bobrow ,  Karl Edwin Adler ,  Kevin Aaron Roth

    IPC分类号： C07C22900

    CPC分类号： C12Q1/28 ,  C07C235/20

    摘要： A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent conmprising an inorganic salt, an organic compound having the formula wherein when X is B(OH)2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; or mixtures of the inorganic salt and the organic compound. A compound having the formula: wherein R1 is —CH2OH, —COOH, and n is 1-8.

    摘要翻译： 通过过氧化物酶增强酚底物向产物的转化的方法包括以下步骤：在增强试剂存在下使包含可检测标记的苯酚的缀合物与过氧化物酶反应，使无机盐， 当X为B（OH）2，Y为I时，或其中当X为OH时，Y为卤素或QR为有机化合物，其中Q为直链或支链1-12杂原子烷基，其中杂原子选自 C，N，O和S，其中连接杂原子烷基链的键是单或双键，其中杂原子烷基链中的任何碳原子任选地包括选自-OH，-COOH，-NH 2和-SH的取代基， 并且其中R选自-OH，-COOH，-NH 2和-CH 3; 或无机盐和有机化合物的混合物。 具有下式的化合物：其中R 1是-CH 2 OH，-COOH，n是1-8。

    Amplified array analysis system
    5.
    发明授权
    Amplified array analysis system 有权
    放大阵列分析系统

    公开(公告)号：US06399299B1

    公开(公告)日：2002-06-04

    申请号：US09430429

    申请日：1999-10-29

    申请人： Mark Norman Bobrow ,  Karl Edwin Adler

    发明人： Mark Norman Bobrow ,  Karl Edwin Adler

    IPC分类号： C12Q168

    CPC分类号： G01N33/54306 ,  C12Q1/6837 ,  C12Q2563/125

    摘要： The present invention concerns an array-based analytical system and method having an enhanced sensitivity which allows for simple and rapid analysis of relative unmodified samples which comprises an analytical system of the type having a plurality of different first members of a specific binding pair affixed in an array thereupon, a mixture including at least one second member of a specific binding pair capable of binding to one of the first members so as to form a specific binding pair which is affixed to the support member, and a reporter system that produces a detectable signal indicative of the presence of the specific binding pair on the support member and wherein the reporter system includes an amplified reporter system that is independent of layering.

    摘要翻译： 本发明涉及一种基于阵列的分析系统和方法，其具有增强的灵敏度，其允许相对未修改的样品的简单和快速分析，其包括具有多个不同的第一成员的分析系统，所述第一成员固定在 阵列，其包含至少一个能够结合到第一个成员之一的特异性结合对的第二个成员的混合物，以形成固定在支持部件上的特异性结合对，以及产生可检测信号的报告系统 指示在支持构件上存在特异性结合对，并且其中报告系统包括独立于分层的扩增报告系统。

    Catalyzed reporter deposition
    7.
    发明授权
    Catalyzed reporter deposition 失效
    催化记者沉积

    公开(公告)号：US5731158A

    公开(公告)日：1998-03-24

    申请号：US651012

    申请日：1996-05-20

    申请人： Mark Norman Bobrow ,  Gerald Joseph Litt

    发明人： Mark Norman Bobrow ,  Gerald Joseph Litt

    IPC分类号： G01N33/532 ,  G01N33/535 ,  G01N33/542 ,  G01N33/58 ,  G01N33/53

    CPC分类号： G01N33/535 ,  G01N33/532 ,  G01N33/542 ,  G01N33/581

    摘要： The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises reacting an analyte dependent enzyme activation system with a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal.

    摘要翻译： 本发明涉及催化报道沉积的方法，以通过扩增检测器信号来改进样品中分析物的检测或定量，所述检测器信号包括使分析物依赖性酶活化系统与由酶系统特异性的可检测标记的底物组成的共轭物反应， 所述缀合物与分析物依赖性酶活化系统反应以形成活化的缀合物，其基本上沉积在活化缀合物的受体固定的任何地方，所述受体不与分析物依赖性酶活化系统反应。 在另一个实施方案中，本发明涉及使用催化报告物沉积来检测或定量样品中分析物的存在或不存在以扩增报道信号的测定。

    Enzymatic substrates for multiple detection systems
    8.
    发明授权
    Enzymatic substrates for multiple detection systems 有权
    用于多个检测系统的酶底物

    公开(公告)号：US08791246B2

    公开(公告)日：2014-07-29

    申请号：US13438029

    申请日：2012-04-03

    申请人： Blas Cerda ,  Mark Norman Bobrow

    发明人： Blas Cerda ,  Mark Norman Bobrow

    IPC分类号： C07C237/22 ,  C07K5/06 ,  C07K5/068 ,  C12Q1/34 ,  C07H15/203 ,  C07K19/00

    CPC分类号： C12Q1/34 ,  C07C237/22 ,  C07H15/203 ,  C07K5/06 ,  C07K19/00 ,  G01N2333/924 ,  G01N2458/15

    摘要： An inventive substrate is provided which includes a substrate compound of formula A-B1-B2-B3-B4: wherein A is a sugar moiety; B1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B2 is a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; B3 contains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and B4 of various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B1-B2-B3-B4, with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity.

    摘要翻译： 提供了本发明的底物，其包括式A-B1-B2-B3-B4的底物化合物：其中A是糖部分; B1是连接部分，允许部分A与底物的剩余结构共轭; B2是具有游离反应性氨基的接头部分，以便可用于与羧酸或可检测标签反应; B3含有永久带电元件如季铵基，以提高质谱分析的灵敏度; 和各种碳长度的B4在检测方法中在各个底物之间赋予特异性。 还提供了式B1-B2-B3-B4的分子，其与通过靶酶在本发明底物上的作用产生的酶产物具有相似的结构特征。 还提供了使用本发明的底物检测酶活性的方法。

    ENZYMATIC SUBSTRATES FOR MULTIPLE DETECTION SYSTEMS
    9.
    发明申请
    ENZYMATIC SUBSTRATES FOR MULTIPLE DETECTION SYSTEMS 有权
    多种检测系统的酶基质

    公开(公告)号：US20120190043A1

    公开(公告)日：2012-07-26

    申请号：US13438029

    申请日：2012-04-03

    申请人： Blas Cerda ,  Mark Norman Bobrow

    发明人： Blas Cerda ,  Mark Norman Bobrow

    IPC分类号： G01N33/573 ,  C12Q1/00 ,  C12Q1/34 ,  C07H15/203

    CPC分类号： C12Q1/34 ,  C07C237/22 ,  C07H15/203 ,  C07K5/06 ,  C07K19/00 ,  G01N2333/924 ,  G01N2458/15

    摘要： An inventive substrate is provided which includes a substrate compound of formula A-B1-B2-B3-B4: wherein A is a sugar moiety; B1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B2 is a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; B3 contains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and B4 of various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B1-B2-B3-B4, with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity.

    摘要翻译： 提供了本发明的底物，其包括式A-B1-B2-B3-B4的底物化合物：其中A是糖部分; B1是连接部分，允许部分A与底物的剩余结构共轭; B2是具有游离反应性氨基的接头部分，以便可用于与羧酸或可检测标签反应; B3含有永久带电元件如季铵基，以提高质谱分析的灵敏度; 和各种碳长度的B4在检测方法中在各个底物之间赋予特异性。 还提供了式B1-B2-B3-B4的分子，其与通过靶酶在本发明底物上的作用产生的酶产物具有相似的结构特征。 还提供了使用本发明的底物检测酶活性的方法。

    Enzymatic substrates for multiple detection systems
    10.
    发明授权
    Enzymatic substrates for multiple detection systems 有权
    用于多个检测系统的酶底物

    公开(公告)号：US08173784B2

    公开(公告)日：2012-05-08

    申请号：US12403790

    申请日：2009-03-13

    申请人： Blas Cerda ,  Mark Norman Bobrow

    发明人： Blas Cerda ,  Mark Norman Bobrow

    IPC分类号： C07H17/02 ,  C08B37/00

    CPC分类号： C12Q1/34 ,  C07C237/22 ,  C07H15/203 ,  C07K5/06 ,  C07K19/00 ,  G01N2333/924 ,  G01N2458/15

    摘要： An inventive substrate is provided which includes a substrate compound of formula A-B1-B2-B3-B4: wherein A is a sugar moiety; B1 is a linker moiety allowing the conjugation of moiety A and the remaining structure of the substrate; B2 is a linker moiety with a free reactive amino group so as to be available for reaction with carboxylic acids or detectable tags; B3 contains a permanently charged element such as a quaternary ammonium group so as to increase sensitivity for mass spectrometry analysis; and B4 of various carbon length conferring specificity amongst individual substrates in detection methods. Also provided is a molecule of the formula B1-B2-B3-B4, with similar structural characteristics to an enzymatic product produced by the action of a target enzyme on an inventive substrate. Further provided are methods for using inventive substrates for detecting enzymatic activity.

    摘要翻译： 提供了本发明的底物，其包括式A-B1-B2-B3-B4的底物化合物：其中A是糖部分; B1是连接部分，允许部分A与底物的剩余结构共轭; B2是具有游离反应性氨基的接头部分，以便可用于与羧酸或可检测标签反应; B3含有永久带电元件如季铵基，以提高质谱分析的灵敏度; 和各种碳长度的B4在检测方法中在各个底物之间赋予特异性。 还提供了式B1-B2-B3-B4的分子，其与通过靶酶在本发明底物上的作用产生的酶产物具有相似的结构特征。 还提供了使用本发明的底物检测酶活性的方法。