公开(公告)号：US20060026719A1

公开(公告)日：2006-02-02

申请号：US11036256

申请日：2005-01-14

申请人： Marcia Kieliszewski ,  Jianfeng Xu

发明人： Marcia Kieliszewski ,  Jianfeng Xu

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/42 ,  C12N15/82 ,  C12N5/04

CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

摘要翻译： 提高重组蛋白质植物表达产量的方法，其包括：使用驱动植物翻译后修饰的密码子将糖基化位点工程化为克隆基因或蛋白质cDNA; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。 该方法导致重组糖基化蛋白质产量增加。 公开了根据这些方法生产的蛋白质。

公开(公告)号：US20060148680A1

公开(公告)日：2006-07-06

申请号：US11036257

申请日：2005-01-14

申请人： Marcia Kieliszewski ,  Jianfeng Xu ,  John Kopchick ,  Shigeru Okada

发明人： Marcia Kieliszewski ,  Jianfeng Xu ,  John Kopchick ,  Shigeru Okada

IPC分类号： A61K38/27

CPC分类号： C07K14/415 ,  A61K38/27 ,  C07K14/56 ,  C07K14/61 ,  C07K14/765 ,  C12N15/62 ,  C12N15/8257 ,  C12P21/005

摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

摘要翻译： 提高重组蛋白质植物表达产量的方法，其包括：使用驱动植物翻译后修饰的密码子将糖基化位点工程化为克隆基因或蛋白质cDNA; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。 该方法导致重组糖基化蛋白质产量增加。 公开了根据这些方法生产的蛋白质。

公开(公告)号：US20070039073A1

公开(公告)日：2007-02-15

申请号：US11173811

申请日：2005-07-01

申请人： Marcia Kieliszewski

发明人： Marcia Kieliszewski

IPC分类号： C07K14/415 ,  A61K38/16 ,  C07H21/04 ,  C12N15/82 ,  C12N5/04

CPC分类号： C07K14/415 ,  C07K2319/00 ,  C12N15/8241 ,  C12N15/8242

摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabino-galactan proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.

摘要翻译： 描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。 在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

公开(公告)号：US20050074838A1

公开(公告)日：2005-04-07

申请号：US10418032

申请日：2003-04-16

申请人： Marcia Kieliszewski

发明人： Marcia Kieliszewski

IPC分类号： A61K38/14 ,  C07H21/04 ,  C07K9/00 ,  C07K14/415 ,  C12N20060101 ,  C12N5/04 ,  C12N15/29 ,  C12N15/82 ,  C07K14/78 ,  C12P21/06

CPC分类号： C07K14/415 ,  C07K2319/00 ,  C12N15/8241 ,  C12N15/8242

摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.

摘要翻译： 描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。 在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

公开(公告)号：US20060252120A1

公开(公告)日：2006-11-09

申请号：US11243295

申请日：2005-09-30

申请人： Marcia Kieliszewski

发明人： Marcia Kieliszewski

IPC分类号： C07H21/04 ,  C12P21/06 ,  C12N5/04 ,  C07K14/415

CPC分类号： C07K14/415

摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.