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    IMPRINTING IN VERY SMALL EMBRYONIC-LIKE (VSEL) STEM CELLS
    7.
    发明申请
    IMPRINTING IN VERY SMALL EMBRYONIC-LIKE (VSEL) STEM CELLS 审中-公开
    注入非常小的类似（VSEL）干细胞

    公开(公告)号：US20120045758A1

    公开(公告)日：2012-02-23

    申请号：US13129352

    申请日：2009-11-16

    申请人： Magdalena Kucia ,  Dong-Myung Shin ,  Mariusz Ratajczak ,  Janina Ratajczak

    发明人： Magdalena Kucia ,  Dong-Myung Shin ,  Mariusz Ratajczak ,  Janina Ratajczak

    IPC分类号： C12Q1/68

    CPC分类号： C12N5/0607 ,  C12Q1/6881 ,  C12Q2600/154

    摘要： Methods for determining a degree of pluripotency in a first putative stem cell relative to a second putative stem cell are provided. In some embodiments the methods include comparing the imprinting status in the first versus the second putative stem cell of a locus selected from among Igf2-H19, Rasgrf1, lgf2R, Kcnq1, and Peg1/Mest. Also provided are methods for distinguishing very small embryonic like (VSEL) stem cells from hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs), methods for isolating VSELs from sources expected to include VSELs, methods for assessing the purity of a very small embryonic like stem cell (VSEL) preparation, and kits that include oligonucleotide primers that can be employed in the practice of the claimed methods.

    摘要翻译： 提供了确定第一推定干细胞相对于第二推定干细胞的多能性程度的方法。 在一些实施方案中，所述方法包括比较选自Igf2-H19，Rasgrf1，IgG2R，Kcnq1和Peg1 / Mest的基因座的第一和第二推定干细胞中的印记状态。 还提供了将非常小的胚胎样（VSEL）干细胞与造血干细胞（HSC）和间充质干细胞（MSC）区分开的方法，从预期包括VSEL的来源分离VSEL的方法，评估非常小的纯度的方法 胚胎样干细胞（VSEL）制剂，以及包括可用于所要求保护的方法的实践中的寡核苷酸引物的试剂盒。

    METHODS AND COMPOSITIONS FOR LONG TERM HEMATOPOIETIC REPOPULATION
    9.
    发明申请
    METHODS AND COMPOSITIONS FOR LONG TERM HEMATOPOIETIC REPOPULATION 审中-公开
    长期HEMATOPOIETIC REPOPULATION的方法和组合

    公开(公告)号：US20120114614A1

    公开(公告)日：2012-05-10

    申请号：US13129359

    申请日：2009-11-16

    申请人： Janina Ratajczak ,  Ewa K. Zuba-Surma ,  Mariusz Ratajczak

    发明人： Janina Ratajczak ,  Ewa K. Zuba-Surma ,  Mariusz Ratajczak

    IPC分类号： A61K35/12 ,  A61N5/00 ,  C12N5/02 ,  C12N5/078 ,  C12N5/07

    CPC分类号： A61K35/28 ,  A61K35/51 ,  A61K2035/124 ,  C12N5/0647 ,  C12N5/0665 ,  C12N2502/1335 ,  C12N2502/1394

    摘要： Methods for isolating a CD133+/CD45neg/GlyAneg subpopulation of umbilical cord blood cells are disclosed. In some embodiments, the methods include providing an initial population of umbilical cord blood cells; contacting the initial population of cells with a first antibody that is specific for CD133, a second antibody that is specific for CD45, and a third antibody that is specific for Glycophorin A (GIyA) under conditions sufficient to allow binding of each antibody to its target, if present, on each cell of the initial population of cells; and isolating a subpopulation of cells that are CD133+, CD45neg, and GlyAneg. Also provided are isolated populations of CD133+/GlyAneg/CD45neg stem cells isolated from cord blood, methods for repopulating cell types in subjects, methods for bone marrow transplantation, methods for inducing hematopoietic competency in CD133+/GlyAneg/CD45neg stem cells, and cell culture systems that include CD133+/GIyAneg/CD45neg stem cells.

    摘要翻译： 公开了分离脐带血细胞的CD133 + / CD45neg / GlyAneg亚群的方法。 在一些实施方案中，所述方法包括提供脐带血细胞的初始群体; 使初始细胞群与第一抗体CD133特异性接触，第二抗体是CD45特异性的第二抗体，以及在足以允许每种抗体与靶标结合的条件下对Glycophorin A（Gly）特异性的第三抗体 如果存在，则在初始细胞群体的每个细胞上; 并分离CD133 +，CD45neg和GlyAneg细胞亚群。 还提供了从脐带血分离的CD133 + / GlyAneg / CD45neg干细胞的分离群体，用于重新培养受试者中的细胞类型的方法，用于骨髓移植的方法，在CD133 + / GlyAneg / CD45neg干细胞中诱导造血能力的方法和细胞培养系统 其包括CD133 + / GlyYneg / CD45neg干细胞。