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    ENZYME-FUNCTIONALIZED SUPPORTS
    5.
    发明申请
    ENZYME-FUNCTIONALIZED SUPPORTS 审中-公开
    酶功能化支持

    公开(公告)号：US20120208256A1

    公开(公告)日：2012-08-16

    申请号：US13503204

    申请日：2010-10-20

    申请人： Ralf Flieg ,  Manuela Klotz ,  Markus Storr ,  Martin Rempfer ,  Wolfgang Freudemann ,  Cornelia Winz ,  Bernd Krause ,  Markus Hornung

    发明人： Ralf Flieg ,  Manuela Klotz ,  Markus Storr ,  Martin Rempfer ,  Wolfgang Freudemann ,  Cornelia Winz ,  Bernd Krause ,  Markus Hornung

    IPC分类号： C12N11/06 ,  C12N11/02

    CPC分类号： C12N9/18 ,  C09D133/14 ,  C12N9/80 ,  C12N11/06 ,  C12N11/08

    摘要： An immobilized enzyme comprises a solid support, an enzyme linked to the solid support and a spacer for coupling the enzyme to the solid support. A method for the production of immobilized enzymes comprises: providing a solid support having amino-functional groups coupled to the support surface; covalently coupling the amino-functional groups with a thermally labile radical initiator; and, contacting the support surface with a solution of polymerizable monomers. The polymerizable monomers comprise functional groups which do not take part in radical polymerization, under conditions where thermally initiated graft copolymerization of the monomers takes place, to form polymer chains on the surface of the support. If the polymer chains do not already comprise primary amino-functional groups, the method further comprises transforming the functional groups in the polymer chains into groups comprising primary amino-functional groups. The method further comprises coupling the enzyme to amino-functional groups of the polymer chains.

    摘要翻译： 固定化酶包括固体支持物，与固体支持物连接的酶和用于将酶偶联到固体支持物的间隔物。 制备固定化酶的方法包括：提供具有与支持表面偶联的氨基官能团的固体支持物; 将氨基官能团与热不稳定的自由基引发剂共价偶联; 并且使支撑体表面与可聚合单体的溶液接触。 可聚合单体包括在发生单体的热引发接枝共聚的条件下不参与自由基聚合的官能团，以在载体的表面上形成聚合物链。 如果聚合物链不包括伯氨基官能团，则该方法还包括将聚合物链中的官能团转化为包含伯氨基官能团的基团。 该方法还包括将酶偶联到聚合物链的氨基官能团。