摘要:
The present invention provides a remedy for a nerve dysfunctional disorder such as a central nervous system damage including a spinal cord injury and a cerebral infarction and the like having an excellent nerve regeneration promoting action which can be administered not only by injecting into a injured site but also by various administration methods including intravenous administration, which can be easily handled and stored over a long time, and can be prepared in a large amount at any time. Said remedy for a nerve dysfunctional disorder such as a central nervous system damage including a spinal cord injury and a cerebral infarction and the like are prepared by using the following as active ingredients: one or more substances selected from a substance secreted from dendritic cells such as IL-12, GM-CSF and the like, a substance inducing and proliferating dendritic cells, a substance activating dendritic cells; a substance inducing the expression of a neurotrophic factor in nerve tissues, a substance inducing and proliferating microglias and macrophages in nerve tissues; and a vector which can expresses the aforementioned substances; or dendritic cell subsets secreting a neurotrophic factor such as NT-3, CNTF, TGF-β1, IL-6, and EGF.
摘要:
It is intended to provide a method of efficiently inducing the growth of nerve stem cells, which are most important in transplantation therapy for nerve damage and neurological dysfunction, either in vitro or in vivo, a method of using the nerve stem cells obtained by the above growth induction method, etc. A mammalian nerve tissue containing nerve stem cells is separated and the nerve stem cells are selectively cultured in a medium containing growth factors such as EGF and FGF. Next, the nerve stem cells are co-cultured with dendritic cell such as an immature dendritic cell subset having a CD11c surface marker on the cell surface, spleen cells or blood cell-type cells such as CD8-positive T cells. Alternatively, the nerve stem cells after the culture are further cultured in the presence of GM-CSF or the nerve stem cells after the culture are further cultured in a culture supernatant of dendritic cells or a culture supernatant of blood cell-type cells.
摘要:
It is intended to provide a diagnosis method and diagnostics for cancers such as human glioma and a therapeutic method and remedies for cancers such as human glioma by identifying tumor suppressive genes or cancer genes useful in diagnosing or treating cancers such as human glioma. Tumor suppressive genes such as RFX1 gene and BGT-1 gene and cancer genes such as HOXD9 gene are screened by comparing a human glioma- or human glioma cell line-origin genomic DNA with a normal tissue-origin genomic DNA in the degree of methylation in CpG island cytosine residues. By targeting these tumor suppressive genes or cancers genes, cancers such as human glioma are diagnosed or treated
摘要:
It is intended to provide highly safe antitumor agents which exhibit an antitumor effect on human remote tumors such as metastatic tumors too and by which an antitumor immune reaction enabling an immune therapy for cancer can be induced, tumor immunity inducers, T cell activators, dendritic cell activators, a method of treating cancer using the same, etc. Inactivated herpes simplex virus (inactivated HSV), herpes simplex virus glycoprotein D (HSVgD), etc. are employed as the active ingredients of antitumor agents, tumor immunity inducers, T cell activators or dendritic cell activators. As a specific example of the treatment for the above-described inactivation, citation may be made of a combination of UV-irradiation using ultraviolet light at 254 nm at 4 J/m2 for 30 minutes with heating at 56° C. for 30 minutes.
摘要翻译:旨在提供高度安全的抗肿瘤剂,其对人类远端肿瘤如转移瘤也具有抗肿瘤作用,通过该抗肿瘤作用可以诱导能够诱导癌症免疫治疗的抗肿瘤免疫反应,肿瘤免疫诱导剂,T细胞激活剂,树突细胞 活化剂,使用其治疗癌症的方法等。使用灭活的单纯疱疹病毒(失活的HSV),单纯疱疹病毒糖蛋白D(HSVgD)等作为抗肿瘤剂,肿瘤免疫诱导剂,T细胞激活剂的活性成分 或树突状细胞激活剂。 作为上述失活的处理的具体例,可以列举使用紫外线照射的组合,使用254nm的紫外线,4J / m 2,30分钟,加热 56℃30分钟。
摘要:
It is intended to provide highly safe antitumor agents which exhibit an antitumor effect on human remote tumors such as metastatic tumors too and by which an antitumor immune reaction enabling an immune therapy for cancer can be induced, tumor immunity inducers, T cell activators, dendritic cell activators, a method of treating cancer using the same, etc. Inactivated herpes simplex virus (inactivated HSV), herpes simplex virus glycoprotein D (HSVgD), etc. are employed as the active ingredients of antitumor agents, tumor immunity inducers, T cell activators or dendritic cell activators. As a specific example of the treatment for the above-described inactivation, citation may be made of a combination of UV-irradiation using ultraviolet light at 254 nm at 4 J/m2 for 30 minutes with heating at 56° C. for 30 minutes.
摘要翻译:旨在提供高度安全的抗肿瘤剂,其对人类远端肿瘤如转移瘤也具有抗肿瘤作用,通过该抗肿瘤作用可以诱导能够诱导癌症免疫治疗的抗肿瘤免疫反应,肿瘤免疫诱导剂,T细胞激活剂,树突状细胞 活化剂,使用其治疗癌症的方法等。使用灭活的单纯疱疹病毒(失活的HSV),单纯疱疹病毒糖蛋白D(HSVgD)等作为抗肿瘤剂,肿瘤免疫诱导剂,T细胞激活剂的活性成分 或树突状细胞激活剂。 作为上述失活的处理的具体例,可以列举使用紫外线照射的组合,使用254nm的紫外线,4J / m 2,30分钟,加热 56℃30分钟。
摘要:
The present invention provides a testis-derived antigenic protein having an immunity induction activity comparable to a glioma antigenic protein and a gene encoding the same, etc., which can be applied to diagnosis and treatment of glioma. A testis-derived antigenic protein having an immunity induction activity comparable to a glioma antigenic protein and a gene encoding the same which can be applied to diagnosis and treatment of glioma are prepared by the steps of: extracting total RNA from testis, synthesizing cDNA, and constructing a λ phage cDNA library by introducing the cDNA into a λ phage vector and infecting Escherichia coli with the vector; reacting thus constructed λ phage cDNA library with the serum of a glioma patient, and using a labeled anti-IgG antibody to detect positive clones that react with the antibody in the serum; repeating the screening process several times on the detected positive clones to confirm the antibody reactivity; and isolating an antigen from the confirmed positive clones and performing serum screening using the isolated antigen and a glioma patient serum and a serum of a healthy individual, etc.
摘要:
The present invention provides a nucleic acid sequence encoding a melanoma antigen recognized by T lymphocytes, designated MART-1. This invention further relates to bioassays using the nucleic acid sequence, protein or antibodies of this invention to diagnose, assess or prognoses a mammal afflicted with melanoma or metastata melanoma. This invention also provides immunogenic peptides derived from the MART-1 melanoma antigen and a second melanoma antigen designated gp100. This invention further provides immunogenic peptides derived from the MART-1 melanoma antigen or gp100 antigen which have been modified to enhance their immunogenicity. The proteins and peptides provided can serve as an immunogen or vaccine to prevent or treat melanoma.
摘要:
The present invention provides a nucleic acid sequence encoding a melanoma antigen recognized by T lymphocytes, designated MART-1. This invention further relates to bioassays using the nucleic acid sequence, protein or antibodies of this invention to diagnose, assess or prognoses a mammal afflicted with melanoma or metastata melanoma. This invention also provides immunogenic peptides derived from the MART-1 melanoma antigen and a second melanoma antigen designated gp100. This invention further provides immunogenic peptides derived from the MART-1 melanoma antigen or gp100 antigen which have been modified to enhance their immunogenicity. The proteins and peptides provided can serve as an immunogen or vaccine to prevent or treat melanoma.
摘要:
The present invention provides a nucleic acid sequence encoding a melanoma antigen recognized by T lymphocytes, designated MART-1. This invention further relates to bioassays using the nucleic acid sequence, protein or antibodies of this invention to diagnose, assess or prognoses a mammal afflicted with melanoma or metastata melanoma. This invention also provides immunogenic peptides derived from the MRT-1 melanoma antigen and a second melanoma antigen designated gp100. This invention further provides immunogenic peptides derived from the MART-1 melanoma antigen or gp100 antigen which have been modified to enhance their immunogenicity. The proteins and peptides provided can serve as an immunogen or vaccine to prevent or treat melanoma.
摘要:
The present invention provides a nucleic acid sequence encoding a melanoma antigen recognized by T lymphocytes, designated MART-1. This invention further relates to bioassays using the nucleic acid sequence, protein or antibodies of this invention to diagnose, assess or prognoses a mammal afflicted with melanoma or metastata melanoma. This invention also provides immunogenic peptides derived from the MART-1 melanoma antigen and a second melanoma antigen designated gp100. This invention further provides immunogenic peptides derived from the MART-1 melanoma antigen or gp100 antigen which have been modified to enhance their immunogenicity. The proteins and peptides provided can serve as an immunogen or vaccine to prevent or treat melanoma.